Collaborative Research: Unlocking the Potential of Active Lipid Vesicles for Directed Delivery and Controlled Release of Therapeutic Payloads

合作研究：释放活性脂质囊泡用于治疗有效负载的定向递送和受控释放的潜力

• 批准号: 2323045
• 负责人: Jie Feng
• 金额: $ 23.4万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2323045&HistoricalAwards=false
• 关键词: Collaborative; Research; Unlocking; Potential; Active

Directed delivery and controlled release of therapeutic payloads at targeted locations present substantial opportunities for developing the next generation of therapeutic delivery systems. Lipid vesicles offer a versatile platform to accomplish both functionalities in a compatible and integrated manner. These lipid vesicles are closed structures with a membrane that separates the interior of the vesicle from the outside environment, similar to the membrane of a biological cell. This award will leverage the innate encapsulation capacity of lipid vesicles to enclose both therapeutic payloads and self-propelled particles, enabling the vesicles to undergo directed motion to targeted locations and controlled release of the encapsulated payloads. Successful development of this active vesicle-based drug delivery system will benefit future healthcare. The award will also provide outreach to K-12 students, especially students from underrepresented groups, to enhance public understanding of the challenges and potential biomedical applications of the project. The proposed research fuses emerging developments of self-propelled (or active) particles with newly discovered vesicle responses to light-induced reactions to create an integrated therapeutic delivery system based on lipid vesicles. By combining experimental and theoretical investigations, this award aims to accomplish three specific objectives. The first objective is to examine the motion of lipid vesicles driven internally by encapsulated active particles, realizing the directed delivery of lipid vesicles to targeted locations. Secondly, a non-contact mechanism triggered by photoreactions will be developed to induce the rupture of a vesicle and thereby the rapid release of its encapsulated content. Finally, the functionalities achieved in the aforementioned objectives will be combined in an in-vitro platform to demonstrate directed delivery and controlled release of therapeutics at targeted locations for relevant biomedical applications via the use of active vesicles. Successful completion of this project will advance fundamental understanding of the complex dynamics of lipid vesicles in these non-equilibrium processes, identifying key physical parameters and design principles to guide subsequent developments of active vesicle-based therapeutic delivery systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

定向交付和受控在有针对性位置的治疗有效载荷的释放为开发下一代的治疗输送系统提供了大量机会。脂质囊泡提供了一个多功能平台，以兼容和集成的方式实现这两个功能。这些脂质囊泡是用膜的封闭结构，将囊泡的内部与外部环境分开，类似于生物细胞的膜。该奖项将利用脂质囊泡的先天封装能力封闭治疗有效载荷和自行性颗粒，使囊泡能够进行定向运动，并控制封装有效载荷。成功开发这种活跃的基于囊泡的药物输送系统将使未来的医疗保健受益。该奖项还将向K-12学生，尤其是来自代表性不足的团体的学生提供宣传，以增强公众对项目的挑战和潜在生物医学应用的了解。 提出的研究融合了新发现的（或活跃）颗粒的新兴发展，新发现的囊泡对光诱导的反应的反应，以基于脂质囊泡的形式创建一个集成的治疗递送系统。通过结合实验和理论研究，该奖项旨在实现三个特定目标。第一个目的是检查由封装的活性颗粒内部驱动的脂质囊泡的运动，以意识到将脂质囊泡的定向输送到靶向位置。其次，将开发由光反应触发的非接触机制来诱导囊泡的破裂，从而快速释放其封装含量。最后，上述目标中实现的功能将在体外平台中合并，以通过使用活性小囊泡在目标位置进行定向输送和受控释放用于相关生物医学应用的治疗方法。该项目的成功完成将进一步了解这些非平衡过程中脂质囊泡的复杂动态，确定关键的物理参数和设计原理，以指导基于囊泡的基于囊泡的治疗交付系统的后续发展。该奖项奖反映了NSF的法定任务，并通过评估该基金会的智力效果，并通过评估了基金会的范围。