Collaborative Research: Chemo-Physics and Molecular Design of In-situ Hydrogel-MXene Biosensors

合作研究：原位水凝胶-MXene生物传感器的化学物理和分子设计

• 批准号: 2320717
• 负责人: Chenglin Wu
• 金额: $ 25.81万
• 依托单位: Missouri University of Science and Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320717&HistoricalAwards=false
• 关键词: Collaborative; Research; Chemo; Physics; Molecular

Colorectal cancer (CRC) has a significant global impact, affecting 2 million people worldwide and standing as the third leading cause of cancer-related deaths in the United States. Technologies that enable in-situ monitoring of biomarkers closely related to the development and progression of CRC are highly desirable for timely diagnosis, early intervention, and personalized treatment of CRC. However, the complicated chemical, biological, and mechanical factors within complex physiological environments present substantial challenges in achieving specific, sensitive, and durable early detection of CRC. With support from the Biosensing Program in the Division of Chemical, Bioengineering, Environmental and Transport Systems and the Chemical Measurement and Imaging Program in the Division of Chemistry, the research groups of Prof. Shaoting Lin (Michigan State University), Prof. Chenglin Wu (Missouri University of Science and Technology), and Prof. Xinyue Liu (Michigan State University) aim to overcome these challenges by developing in-situ hydrogel-MXene biosensors capable of detecting low-level CRC biomarkers (e.g., carcinoembryonic antigen (CEA)) in simulated intestinal environments. The in-situ hydrogel-MXene biosensor will potentially lead to a big leap in bioelectronics, offering transformative impacts in disease diagnostics and paving the way for personalized healthcare. In addition, this project is expected to foster the development of the next-generation workforce in emerging biotechnologies through multi-institutional and interdisciplinary efforts including the integration of new topics in undergraduate courses and the promotion of STEM fields to encourage more students to engage in these areas of study.The goal of this project is to integrate selective-permeable hydrogels with MXene-based field-effect transistors for developing high-performance hydrogel-MXene biosensors that can achieve in-situ detection of low-level colorectal cancer (CRC) biomarkers (e.g., carcinoembryonic antigen (CEA)) in simulated intestinal environments while mitigating the influence of mobile ions. To achieve this, this project will leverage molecular design of reversible interaction, network topology, and fixed charge in hydrogels to modulate hydrogel-intestine interactions for selective biomolecular transport and to regulate hydrogel-MXene interactions for improved field-effect sensing performance. Specifically, this project will leverage the synergy of network elasticity and reversible interaction in hydrogels to independently modulate the transport of target and non-target biomolecules, thereby enabling selective and enhanced transport. In addition, this project will harness the combined efforts of molecular design, atomic simulation, and DFT calculation to optimize the design of network topology and fixed charge density of hydrogels, thus maximizing the Debye length, reduce the capacitance, and tune the bandgap at the hydrogel-MXene interface. Finally, this project will incorporate the explored hydrogel design principles to build a high-performing hydrogel-MXene biosensor capable of quantifying CRC biomarkers in simulated intestinal environments.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

大肠癌（CRC）具有重大的全球影响力，在全球影响200万人，是美国与癌症相关死亡的第三大主要原因。对于及时的诊断，早期干预和对CRC的个性化治疗，对与CRC的发展和进展密切相关的生物标志物的原位监测的技术非常可取。然而，复杂的化学，生物学和机械因素在复杂的生理环境中提出了实质性挑战，可以实现特定，敏感和持久的CRC检测。 With support from the Biosensing Program in the Division of Chemical, Bioengineering, Environmental and Transport Systems and the Chemical Measurement and Imaging Program in the Division of Chemistry, the research groups of Prof. Shaoting Lin (Michigan State University), Prof. Chenglin Wu (Missouri University of Science and Technology), and Prof. Xinyue Liu (Michigan State University) aim to overcome these challenges by developing in-situ hydrogel-MXene biosensors能够在模拟的肠道环境中检测低水平的CRC生物标志物（例如，癌胚抗原（CEA））。原位水凝胶 - 氧化生物传感器将有可能导致生物电子学的巨大飞跃，从而在疾病诊断中产生变革性的影响，并为个性化的医疗保健铺平道路。此外，该项目有望通过多个机构和跨学科的努力来促进新兴生物技术的下一代劳动力的发展，包括在本科课程中成立新主题，并促进STEM领域的促进领域，以促进更多的学生参与这些领域。可以在模拟的肠道环境中实现低水平结直肠癌（CRC）生物标志物（例如Carcineembryonic抗原（CEA））的水凝胶 - 氧化生物传感器，同时缓解移动离子的影响。为了实现这一目标，该项目将利用可逆相互作用，网络拓扑和水凝胶中的固定电荷的分子设计，以调节水凝胶 - 刻板的相互作用，以进行选择性生物分子转运，并调节水凝胶 - 含量相互作用，以改善现场效应感测性能。具体而言，该项目将利用水凝胶中网络弹性和可逆相互作用的协同作用，以独立调节目标和非目标生物分子的运输，从而实现选择性和增强的运输。此外，该项目将利用分子设计，原子模拟和DFT计算的综合努力，以优化水凝胶的网络拓扑设计和固定电荷密度，从而最大程度地提高Debye的长度，减少电容，并在水凝胶 - 墨西界面上调整带隙。最后，该项目将纳入探索的水凝胶设计原理，以建立能够在模拟的肠环境中量化CRC生物标志物的高性能水凝胶生物传感器。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子的智力和广泛影响来评估的，并被视为值得通过评估的支持。