I-Corps: Commercialization of a real-time, palm-size, low-cost, and wireless SARS-COV-II detector

I-Corps：实时、手掌大小、低成本、无线 SARS-COV-II 探测器的商业化

• 批准号: 2230853
• 负责人: Zheng Tian
• 金额: $ 5万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2230853&HistoricalAwards=false
• 关键词: Corps; Commercialization; real; time; palm

The broader impact/commercial potential of this I-Corps project is the development of a wireless nano-biosensor for detection of live coronavirus particles and potentially other pathogens. Currently, a positive result from an enzyme-linked immunosorbent assay (ELISA)- or polymerase chain reaction (PCR)-based COVID test does not determine if the coronavirus particles are live (infectious) or dead (or neutralized or noninfectious). The proposed technology is designed to detect interactions of live coronavirus particles with target cells, antibodies, and drugs in real-time. Those with a negative result from the proposed technology have confirmation that they are free of live, infectious coronavirus particles and would not need to quarantine. This technology may provide a tool for effectively combatting the COVID-19 virus and future pandemics/epidemics as well as other infections.This I-Corps project is based on the development of a nano-biosensing methodology for pathogen detection initially focused on SARS COV-II. The proposed technology integrates a bio-ceramic nanowire/graphene core/shell nanocomposite with a radio frequency identification (RFID) tag on a wireless communication chip surface. The hardware of the nano-biosensing system is composed of three parts: a radio frequency nano-sensor, a radio frequency reader, and a handheld device or smart phone. The proposed nano-biosensor is made by coating the biosafe nanocomposite on a disposable RFID tag to maximize the sensitivity. Further, nano-biosensors may be integrated to simultaneously detect samples in a multi-well plate, which is versatile and effective in the detection of virus particles, viral antigens, and antibodies, as well as in evaluating virus-neutralizing antibodies and vaccine efficacy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 I-Corps 项目更广泛的影响/商业潜力是开发用于检测活冠状病毒颗粒和潜在其他病原体的无线纳米生物传感器。目前，基于酶联免疫吸附测定 (ELISA) 或聚合酶链反应 (PCR) 的新冠病毒检测的阳性结果并不能确定冠状病毒颗粒是活的（传染性的）还是死的（或中和的或非传染性的）。 该技术旨在实时检测活冠状病毒颗粒与靶细胞、抗体和药物的相互作用。那些通过拟议技术获得阴性结果的人已确认他们没有活的传染性冠状病毒颗粒，并且不需要隔离。这项技术可能为有效对抗 COVID-19 病毒和未来的流行病/流行病以及其他感染提供一种工具。该 I-Corps 项目基于开发一种用于病原体检测的纳米生物传感方法，最初专注于 SARS COV-二. 该技术将生物陶瓷纳米线/石墨烯核/壳纳米复合材料与无线通信芯片表面的射频识别（RFID）标签集成在一起。 纳米生物传感系统的硬件由射频纳米传感器、射频读取器和手持设备或智能手机三部分组成。所提出的纳米生物传感器是通过将生物安全纳米复合材料涂覆在一次性 RFID 标签上制成的，以最大限度地提高灵敏度。此外，可以集成纳米生物传感器以同时检测多孔板中的样品，这在检测病毒颗粒、病毒抗原和抗体以及评估病毒中和抗体和疫苗功效方面具有通用性和有效性。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。