Graphene/Polymer-based Fluorescent Nanozymes for Sensitive Detection of Metabolic Biotargets

用于代谢生物靶标灵敏检测的石墨烯/聚合物荧光纳米酶

• 批准号: 2304873
• 负责人: Julia Xiaojun Zhao
• 金额: $ 45万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2304873&HistoricalAwards=false
• 关键词: Graphene; Polymer; based; Fluorescent; Nanozymes

With support from the Chemical Measurement and Imaging (CMI) program in the Division of Chemistry, and co-funding from the Established Program to Stimulate Competitive Research (EPSCoR), the Chemical Structures, Dynamics, and Mechanisms – B (CSDM-B) program in the Chemistry Division, and the Biosensing Program in the Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET), Professors Julia Zhao and Diane Darland and their respective research groups at the University of North Dakota are devising a new approach to nanomaterial-based sensors, known as nanozymes, for the detection and quantitation of key metabolic biomolecules, such as glucose. They seek to create sensors usable in a range of environments and characterized by high sensitivity, easy synthesis, and good biocompatibility. In conjunction with this work, Drs. Zhao and Darland are reaching out to engage tribal high schools and colleges as well as other universities in the North Dakota University System, to encourage students from groups underrepresented in STEM (science, technology, engineering and mathematics) to consider science as a career option. Additional efforts are underway to develop hands-on advanced science lessons to help middle- and/or high-school students connect concepts from atoms to ecosystems with nanomaterials and cells. Under this award, the collaborative Zhao/Darland team at the University of North Dakota will work to develop graphene- and polymer-based fluorescent nanozymes for sensitive detection of metabolic biomolecules. The nanozymes co-catalyze targeted biomolecular reactions while providing an intense fluorescence signal proportional to the analyte concentration. Targeted attributes include high stability in various operational environments, high detection sensitivity, easy synthesis, good biocompatibility, and applicability for detection of critical cellular energetic targets. In the course of these studies, the team seeks a better understanding of the geometric, compositional, dimensional, and electrochemical characteristics of graphene- and polymer-based nanozymes. Nanozymes will be tested for toxicity and used for biotarget detection and cell imaging. Cells that display high metabolic activity and that are vulnerable to metabolic distress will be used for testing the nanozymes under normal and distressed metabolic conditions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

借助化学测量和成像（CMI）在化学划分中的支持，并从既定计划中共同基金，以刺激竞争性研究（EPSCOR），化学结构，动力学和机制 - B（CSDM-B）在化学部门中的计划，以及化学，BioEsmensing计划，以及化学，BioEsmensing Programe of Chemical，BioEsmensing Programe of Chemical，BioEnsering Systems and Envirencemeling Zhaer and Envirencemal和Environment Zhha（CBER）（CBET），CBET（CBET），CBET（CBET），CBET（CBET） Darland及其各自的研究小组在北达科他大学（University of North Dakota）正在为纳米材料的传感器（称为纳米酶）设计一种新方法，用于检测和定量关键代谢生物分子，例如葡萄糖。他们试图创建在各种环境中可用的传感器，并以高灵敏度，易于合成和良好的生物相容性为特征。博士，与这项工作一起。 Zhao和Darland正在接触到北达科他大学系统中的部落高中和学院以及其他大学，以鼓励来自STEM（科学，技术，工程和数学）人数不足的小组的学生将科学视为职业选择。正在进行额外的努力，以开发动手高级科学课程，以帮助中学和/或高中生通过纳米材料和细胞将概念与生态系统联系起来。该奖项是，北达科他大学的Zhao/Darland团队将致力于开发基于石墨烯和聚合物的荧光纳米酶，以敏感地检测代谢生物分子。纳米人共催化靶向的生物分子反应，同时提供与分析物浓度成比例的强烈荧光信号。有针对性的属性包括在各种操作环境中的高稳定性，高检测灵敏度，易于合成，良好的生物相容性以及用于检测关键细胞能量目标的适用性。在这些研究的过程中，团队寻求更好地了解基于石墨烯和聚合物纳米的几何，成分，尺寸和电化学特征。纳米酶将用于毒性，并用于生物目标检测和细胞成像。表现出高代谢活性且容易遭受代谢困扰的细胞将用于在正常和遇险的代谢状态下测试纳米因素。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响标准来评估NSF的法定任务。