Excellence in Research: Unravelling the Genetic Basis for Heavy-Metal Uptake and Tolerance in Nerium oleander Through Transcriptomic Kaleidoscope

卓越的研究：通过转录组万花筒揭示夹竹桃重金属吸收和耐受性的遗传基础

• 批准号: 2247625
• 负责人: Naira Ibrahim
• 金额: $ 88万
• 依托单位: Jackson State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2247625&HistoricalAwards=false
• 关键词: Excellence; Research; Unravelling; Genetic; Basis; Excellence; Research; Unravelling; Genetic; Basis

The understanding of the genetic basis for plants' uptake and tolerance of heavy metals remains limited. This study aims to investigate the mechanisms by which Nerium oleander, commonly known as oleander, absorbs and withstands heavy metals in its surroundings. By analyzing the plant's genetic composition and investigating gene activation and suppression, scientists seek to unravel the mechanisms that enable Nerium oleander to thrive in polluted areas. This research holds societal relevance as it tackles environmental concerns such as pollution and its impact on ecosystems. What sets this research apart is its innovative approach of employing transcriptomics, which involves studying gene activity, to gain insights into how Nerium oleander copes with heavy-metal contamination. By elucidating the genetic foundation of heavy-metal uptake and tolerance, this study could have far-reaching implications for ecological restoration, phytoremediation (the use of plants to remediate polluted sites), and sustainable agricultural practices. This project thus, represents a significant scientific endeavor that could yield valuable knowledge to address urgent environmental challenges. Overall, this research contributes to the advancement of scientific understanding, offers support for sustainable solutions, and promotes the well-being of our environment and society.The Genetic Mechanisms that regulate heavy-metal uptake and tolerance in plants is poorly understood. This study aims to explore the genetic basis behind Nerium oleander's remarkable ability to absorb and withstand heavy metals in contaminated environments. Specifically, the study seeks to investigate how Nerium oleander can effectively take in and endure heavy metals like cadmium, lead, and zinc without experiencing detrimental effects. The primary objectives of this research include unraveling the molecular pathways and gene regulatory networks that contribute to heavy-metal uptake and tolerance in this plant species. To achieve these goals, a comprehensive transcriptomic analysis will be conducted. RNA samples from plants subjected to heavy-metal stress, as well as control conditions, will be sequenced, enabling the identification and quantification of gene expression patterns. By comparing the transcriptomes of stressed and non-stressed plants, differentially expressed genes associated with heavy-metal uptake and tolerance will be discovered. The project will involve functional enrichment analysis, aiming to identify key transcription factors and signaling pathways involved in the plant's response to heavy-metal stress. By integrating diverse data sets and employing various biological approaches, a comprehensive understanding of the genetic mechanisms underlying heavy-metal uptake and tolerance in Nerium oleander will be achieved. The findings of this research will not only contribute to the broader field of plant stress responses but also provide a foundational understanding of the mechanisms involved in phytoremediation—the use of plants to mitigate pollution. This study represents a crucial step towards unraveling the genetic complexities of heavy-metal tolerance in plants and may pave the way for future advancements in sustainable environmental remediation strategies.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

对植物摄取和重金属耐受性的遗传基础的理解仍然有限。这项研究旨在研究北毛夹层（通常称为夹骨）的机制，在其周围环境中吸收并承受重金属。通过分析植物的遗传组成并研究基因激活和抑制，科学家试图揭示能够在污染区域繁殖的机制。这项研究具有社会意义，因为它解决了诸如污染及其对生态系统的影响之类的环境问题。这项研究与众不同的是，其使用转录组学的创新方法涉及研究基因活性，以深入了解nerium leander如何应对重量金属污染。通过阐明重金属摄取和耐受性的遗传基础，这项研究可能对生态恢复，植物修复（使用植物来补救污染地点）和可持续的农业实践具有深远的影响。因此，该项目代表了一项重大的科学努力，可以产生宝贵的知识来应对紧急环境挑战。总体而言，这项研究有助于发展科学理解，为可持续解决方案提供支持，并促进我们环境和社会的福祉。众所周知，调节重金属摄取和耐受性的遗传机制知之甚少。这项研究旨在探索Nerium leander在受污染环境中吸收和承受重金属的显着能力背后的遗传基础。具体而言，该研究旨在研究Nerium夹骨如何有效地摄入并忍受镉，铅和锌等重金属，而不会遇到不利影响。这项研究的主要目标包括阐明有助于这种植物物种的重量摄取和耐受性的分子途径和基因调节网络。为了实现这些目标，将进行全面的转录组分析。将对遭受重量应激的植物以及对照条件的RNA样品进行测序，从而鉴定和定量基因表达模式。通过比较压力和非压力植物的转录组，将发现与重量摄取和耐受性相关的不同表达基因。该项目将涉及功能富集分析，旨在确定植物对重金属应力的反应中涉及的关键转录因子和信号通路。通过整合潜水员的数据集并采用各种生物学方法，将实现对重量金属摄取和耐受性的遗传机制的全面理解。这项研究的发现不仅会有助于更广泛的植物压力反应领域，而且还为植物修复所涉及的机制提供了基本的理解，即使用植物来减轻污染。这项研究代表了探讨植物中重量耐受性的遗传复杂性迈出的至关重要的一步，并可能为未来的可持续环境修复策略的进步铺平了道路。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的智力和更广泛影响的评估来审查审查的审查批评，这是值得通过评估的支持。