PFI-TT: Platform Technology for Bioengineering Applications of Large mRNA

PFI-TT：大mRNA生物工程应用的平台技术

• 批准号: 2234570
• 负责人: Yupeng Chen
• 金额: $ 25万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2234570&HistoricalAwards=false
• 关键词: PFI; TT; Platform; Technology; Bioengineering

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project will introduce an innovative and more effective option to help large mRNA enter cartilage and joint tissues for a variety of biomedical applications from therapeutics to research reagents. With the recent success of COVID-19 vaccines, mRNA-based vaccines and therapeutics have attracted substantial interest from both academia and the biopharmaceutical industry. However, the delivery of large mRNA is still a major challenge. The proposed platform technology presents many advantages over conventional delivery vehicles. PhD students and undergraduates will receive training in technology development and commercialization. The proposed project will develop a delivery technology based on the innovative DNA-inspired Janus base nanomaterials (JBNs) for large mRNA that addresses a critical market need for applications in “difficult-to-penetrate” cartilage tissue for joint and skeletal diseases. The delivery of large mRNA is still a major challenge. The proposed platform technology presents many advantages over conventional delivery vehicles such as lipid nanoparticles and polymers. This new technology has superior ability to penetrate into cartilage and other joint tissues, has significantly better endosomal escape ability than lipid nanoparticles, and has significant lower cytotoxicity compared to cationic polymers and lipids due to their noncovalent structures and DNA-mimicking chemistry. Furthermore, this new delivery technology has tremendous versatility. Depending on the specific needs of a customer, the technology can be tailored for different joint and skeletal diseases.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该创新合作伙伴关系 - 技术转化 (PFI-TT) 项目的更广泛影响/商业潜力将引入一种创新且更有效的选择，帮助大 mRNA 进入软骨和关节组织，用于从治疗到研究试剂的各种生物医学应用。最近，COVID-19 疫苗、基于 mRNA 的疫苗和疗法的成功引起了学术界和生物制药行业的极大兴趣，然而，大 mRNA 的递送仍然是一个重大挑战。博士生和本科生将接受技术开发和商业化方面的培训，该项目将开发一种基于创新型 DNA 启发的 Janus 基础纳米材料 (JBN) 的大型 mRNA 递送技术，以满足 mRNA 的关键市场需求。用于治疗关节和骨骼疾病的“难以穿透”的软骨组织仍然是一个重大挑战，所提出的平台技术比脂质纳米粒子和聚合物等传统的递送载体具有许多优势。具有优异的渗透软骨和其他关节组织的能力，比脂质纳米颗粒具有明显更好的内体逃逸能力，并且由于其非共价结构和模拟 DNA 化学，与阳离子聚合物和脂质相比，其细胞毒性显着降低。具有巨大的多功能性，根据客户的具体需求，该技术可以针对不同的关节和骨骼疾病进行定制。该奖项反映了 NSF 的法定使命，并通过使用评估被认为值得支持。基金会的智力价值和更广泛的影响审查标准。

项目成果

Blood-brain-barrier modeling with tissue chips for research applications in space and on Earth

• DOI: 10.3389/frspt.2023.1176943
• 发表时间: 2023-08-09
• 期刊: FRONTIERS IN SPACE TECHNOLOGIES
• 作者: Yau，Anne;Jogdand，Aditi;Chen，Yupeng
• 通讯作者: Chen，Yupeng

Nanomedicine strategies for central nervous system (CNS) diseases

• DOI: 10.3389/fbiom.2023.1215384
• 发表时间: 2023-08
• 期刊: Frontiers in biomaterials science
• 作者: Shreya Nagri;Olivia Rice;Yupeng Chen