RAPID: Diversity and symbiotic relationships of annelids in a poorly known region of the deep Pacific

RAPID：太平洋深部鲜为人知的地区环节动物的多样性和共生关系

• 批准号: 2231299
• 负责人: Kenneth Halanych
• 金额: $ 10.78万
• 依托单位: University of North Carolina at Wilmington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2231299&HistoricalAwards=false
• 关键词: RAPID; Diversity; symbiotic; relationships; annelids

While the oceans occupy more than 70% of the Earth’s surface, the diversity of the deep-sea is still vastly understudied due to the difficult and expensive nature of sampling. Based on the limited sampling of the deep seafloor, researchers have found that biodiversity in these environments is dependent upon factors, such as temperature, salinity, geomorphology, and depth. One area lacking data is oceanic trenches which sometimes reach depths over 7,000m. Given the environmental extremes of oceanic trenches, they may harbor unique sets of species but biodiversity from these regions is almost completely unknown. Annelids, or segmented worms, are a diverse and dominant representative of communities in marine environments, including deep-sea habitats. However, limited sampling from the deep sea has restricted our ability to characterize annelid biodiversity from these widespread environments. In addition to exploring, describing and cataloging the biodiversity of annelid worms from the deep sea, this project will result in two new outreach activities. In collaboration with the MarineQuest program at the University of North Carolina at Wilmington, two new outreach activities for K-12 students will be developed to illustrate life aboard a research vessel and how researchers study adaptations of organisms to life in the deep sea. This project will collect annelids from hadal sites of the Eastern Aleutian Trench in the Northeast Pacific Ocean and abyssal depths of the nearby continental shelf in the Bering Sea aboard the German R/V Sonne under the leadership of Dr. Angelika Brandt of The Senckenberg Research Institute and Natural History Museum. The samples will be preserved in formalin and RNAlater for morphological and molecular work, respectively. The goal is to document and describe annelid diversity and their symbionts from these remote and extreme environments using morphological and molecular sequencing techniques. Traditional taxonomy, fluorescence in-situ hybridization (FISH), and -omics techniques will be used to characterize annelid diversity in this relatively unstudied region of the deep-sea and determine if there is a greater abundance of symbiotic taxa in annelid microbiomes at hadal depths compared to abyssal depths.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

尽管海洋占地70％以上，但由于采样的困难和昂贵的性质，深海的多样性仍然被广泛理解。基于深海深处的采样有限，研究人员发现，这些环境中的生物多样性取决于温度，盐度，地貌和深度等因素。缺乏数据的一个区域是海洋沟，有时达到7,000m的深度。鉴于海洋沟的环境极端，它们可能藏有独特的物种，但是这些地区的生物多样性几乎是完全未知的。 Annelids或分割的蠕虫是海洋环境中社区（包括深海栖息地）的潜水员和主导代表。但是，深海的有限抽样限制了我们从这些宽度环境中表征Annelid生物多样性的能力。除了探索，描述和分类深海的Annelid蠕虫的生物多样性外，该项目还将导致两项新的外展活动。将与北卡罗来纳大学威尔明顿大学的海军陆战队计划合作，将开发针对K-12学生的两项新的外展活动，以说明研究船上的生活以及研究人员如何研究生物体对深海生活的适应。该项目将从东北太平洋东部阿留申沟的Hadal地点收集Annelids，并在Senckenberg研究所和自然历史博物馆的Angelika Brandt博士的领导下，在德国R/V Sonne的近乎盛大架子的深处。样品将分别保存在福尔马林和rnalater中，分别用于形态学和分子工作。目的是使用形态和分子测序技术记录和描述来自这些遥远和极端环境的Annelid多样性及其符号。传统的分类法，荧光原位杂交（鱼）和 - 组技术将用于表征深海的相对未研究区域中的annelid多样性，并确定与在hadal decters and sater ne sater nesf的范围内相比，Annelid Microbobiomes中的共生分类是否更大。使用基金会的智力优点和更广泛的影响评估标准进行评估。