I-Corps: High-throughput screening platform for complex visual properties

I-Corps：复杂视觉特性的高通量筛选平台

• 批准号: 2230599
• 负责人: Francois St-Pierre
• 金额: $ 5万
• 依托单位: Baylor College of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2230599&HistoricalAwards=false
• 关键词: Corps; throughput; screening; platform; complex

The broader impact/commercial potential of this I-Corps project is the development of a technology that enables rapid and cost-effective engineering of proteins and cells. The technology has the potential to accelerate the development of many types of biological products such as protein-based biosensors, enzymes, therapeutic proteins, and genetic circuits. The technology could results in more environmentally-friendly methods of producing existing materials and faster development of pharmaceuticals. The bio-pharma industry suffers from slow, expensive, and risky development of therapeutics and diagnostics; The drugs that reach the market often require a lengthy discovery and development process that takes over a decade with an average cost of $1-2 billion. This platform seeks to develop bio-products more efficiently.This I-Corps project seeks to develop a microscopy-based bioengineering platform that can design spatial and temporal properties of proteins and cells at unprecedented speed. This platform may increase the speed of innovation by several magnitudes using custom computer vision software and a light-mediated, single-cell retrieval method to conduct single-cell level screening. The screening process and analyses can be tuned to fit the needs of a specific workflow in different experimental contexts. The platform's utility is demonstrated by optimizing fluorescent protein probes, common visualization tools used by biologists. Using this platform, millions protein variants are tested and variants are identified with the most stable fluorescence output.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该I-Corps项目的更广泛的影响/商业潜力是开发一种技术，该技术可以快速且具有成本效益的蛋白质和细胞工程。该技术有可能加速许多类型的生物产品，例如基于蛋白质的生物传感器，酶，治疗蛋白和遗传回路。该技术可能会导致更环保的方法来生产现有材料并更快地开发药品。生物制度行业患有缓慢，昂贵且冒险的治疗学和诊断症。到达市场的药物通常需要一个漫长的发现和开发过程，该过程需要十多年的时间，平均成本为10-2亿美元。该平台试图更有效地开发生物产品。该I-Corps项目旨在开发基于显微镜的生物工程平台，该平台可以以前所未有的速度设计蛋白质和细胞的时空特性。该平台可以使用自定义的计算机视觉软件和一种光介导的单细胞检索方法来提高几个大小的创新速度，以进行单细胞级筛选。可以调整筛选过程和分析以适合不同实验环境中特定工作流程的需求。通过优化荧光蛋白探针，生物学家使用的常见可视化工具来证明该平台的实用程序。使用此平台，测试了数百万个蛋白质变体，并以最稳定的荧光输出确定了变体。该奖项反映了NSF的法定任务，并且使用基金会的智力优点和更广泛的影响评估标准，认为值得通过评估来获得支持。