NSF-sponsored workshop: The cross-disciplinary study of post-transcriptional and post-translational modifications: Finding the commonalities of interests, approaches, and future di

NSF 赞助的研讨会：转录后和翻译后修饰的跨学科研究：寻找兴趣、方法和未来发展的共同点

• 批准号: 2135914
• 负责人: Brian Gregory
• 金额: $ 5.27万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2135914&HistoricalAwards=false
• 关键词: NSF; sponsored; workshop; cross; disciplinary

Every organism on earth relies on its DNA content (i.e., genome) to direct its life, growth, and development. However, the genome is not the destiny of the organism. For example, caterpillars and butterflies have exactly the same genome, but distinct forms. The difference can be largely attributed to the other important biomolecules that are encoded by the genome. These biomolecules are RNA and proteins, which can be modified in hundreds of different ways after their synthesis. Recognition of the diversity and complexity of modifications of RNA and proteins has changed how we think about their different functions of these molecules in fundamental ways in both large and small organisms, ranging from microbes to plants and humans. Understanding how RNA and proteins are modified, and their biological implications is very important in many areas of basic and applied biological and medical research. Improving our understanding will require building community resources, technical breakthroughs, and cross-disciplinary research efforts to achieve cross-cutting advances in RNA and protein biology. This project will support a virtual national workshop that will bring together 100-150 diverse participants that are actively involved in both the meeting and discussion sessions. In addition, all research talks, and discussion sessions will be open for anyone interested to watch through Zoom. The participants are from many different disciplines and areas, including plant, animal, and human biology, and cover research areas such as chemistry, engineering, computer science, artificial intelligence, and systems and synthetic biology. Keynote talks from leaders, small-group and interactive sessions will facilitate and stimulate researchers to address the following key topics: 1) Successes and failures in RNA and protein modification research in a variety of research organisms; 2) Experimental systems as well as research approaches that leverage all RNA and protein–related scientific disciplines; 3) Possible “rules of life” to guide baseline and comparative questions across the different realms of organisms; 4) Advantages and disadvantages of currently available tools, consortia, and databases; 5) Training needs for researchers capable of contributing to future advancement in RNA and protein biology; and 6) Short- and medium-term research and knowledge goals, noting potential strategies to reach these goals.In the framework of our current understanding of genetic information flow, post-transcriptional modifications (PRMs) of RNA molecules and post-translational modifications (PTMs) of proteins are extremely important cellular regulatory moieties. Yet significant gaps remain in the integrated understanding of these two regulatory moieties and their effects on overall cellular metabolism in different biological organisms. Fragmentated research efforts in different systems (e.g., microbe, plant, animal, and human) and using different approaches and standards, has prevented integrative analyses of different datasets and identification of common principles of PRMs and PTMs. This workshop aims to fill these gaps and advance research from descriptive and specific mechanistic focus towards integration, general theory, modeling and prediction, and global understanding. Moreover, this workshop will promote studying the functions and mechanisms of the key regulatory modifications, of their roles in cellular signaling and metabolism, as well as their impact on organisms. The workshop will provide significant opportunities for cross-disciplinary interactions to advance the field of RNA and protein biology by identifying: 1) core questions driving/motivating PRM and PTM studies; 2) conceptual and theoretical foundations that provide a useful lens for hypothesis testing; 3) big ideas to drive advances in these areas and increased understanding of the rules of life; and 4) a path forward for collaborative research and synthetic analyses that will further integrate the two closely-related areas of research through multidisciplinary approaches. Early-career post-doctoral scientists and graduate students will participate in the workshop organization and serve as virtual discussion leaders, providing them the opportunity to network with many of the leaders in these cutting-edge fields. The workshop aims to increase broad participation of underrepresented groups in RNA and protein modification research, and serves as a model for integration of inclusive and interactive online conference opportunities allowing participation from broader audiences. Collectively, this workshop will identify critical gaps in resources and understanding of PRMs and PTMs across disciplines, and importantly draft a path forward for community-wide collaborative research to advance these important areas of research that affect our understanding of human, plant, animal, and ecosystem biology. Funding for this workshop was provided by the Physiological and Structural Systems Cluster, Plant Genome Research Program, and Genetic Mechanisms Cluster in the BIO Directorate and the Cellular and Biochemical Engineering Program in the ENG Directorate.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

地球上的每个生物体都依赖其 DNA 内容（即基因组）来指导其生命、生长和发育，但是，基因组并不是生物体的命运，例如，毛毛虫和蝴蝶具有完全相同的基因组，但又不同。这种差异很大程度上归因于基因组编码的其他重要生物分子，这些生物分子在合成后可以以数百种不同的方式进行修饰。和蛋白质有从微生物到植物和人类，从根本上改变了我们对这些分子不同功能的看法，了解 RNA 和蛋白质的修饰方式以及它们的生物学意义在许多基础和研究领域非常重要。提高我们对应用生物学和医学研究的理解需要建立社区资源、技术突破和跨学科研究努力，以实现 RNA 和蛋白质生物学的跨领域进展。该项目将支持一个虚拟的国家研讨会，该研讨会将汇集 100 名专家。 150 名不同的参与者积极参与此外，所有研究演讲和讨论会议都将开放给任何有兴趣的人通过 Zoom 观看，参与者来自许多不同的学科和领域，包括植物、动物和人类生物学。化学、工程、计算机科学、人工智能、系统和合成生物学等研究领域的领导者的主题演讲、小组和互动会议将促进和激励研究人员讨论以下关键主题：1) RNA 的成功和失败。以及各种研究生物体中的蛋白质修饰研究； 2) 利用所有 RNA 和蛋白质相关科学学科的实验系统和研究方法； 3) 指导不同生物领域的基线和比较问题的可能“生命规则”； 4) 当前可用工具的优点和缺点； 、联盟和数据库；5) 对能够为 RNA 和蛋白质生物学的未来发展做出贡献的研究人员的培训需求；以及 6) 短期和中期研究和知识目标，指出实现这些目标的潜在策略。就我们目前对遗传信息流的理解而言，RNA分子的转录后修饰（PRM）和蛋白质的翻译后修饰（PTM）是极其重要的细胞调节部分，然而对这两个调节部分的综合理解仍然存在显着差距。它们对不同生物有机体整体细胞代谢的影响，在不同系统（例如微生物、植物、动物和人类）中的分散研究工作以及使用不同的方法和标准，阻碍了对不同数据集和识别的综合分析。本研讨会旨在填补这些空白，并将研究从描述性和具体机制重点转向整合、一般理论、建模和预测以及全球理解。此外，本研讨会将促进对 PRM 和 PTM 的功能和机制的研究。该研讨会将为跨学科互动提供重要机会，通过确定以下内容来推进 RNA 和蛋白质生物学领域的发展：1) 驱动的核心问题。 /激励 PRM 和 PTM 研究； 2）为假设检验提供有用的概念和理论基础；3）推动这些领域进步并增进对生命规则的理解的伟大想法；4）进一步整合的合作研究和综合分析的前进道路；通过多学科方法进行两个密切相关的研究领域，早期职业博士后科学家和研究生将参加研讨会组织并担任虚拟讨论领导者，为他们提供与这些前沿领域的许多领导者建立联系的机会。研讨会旨在增加边缘领域的广泛参与。总的来说，该研讨会将确定跨学科资源和 PRM 和 PTM 理解方面的关键差距，并作为包容性和交互式在线会议机会整合的模型，让更广泛的受众参与其中。为全社区合作研究制定一条前进道路，以推进影响我们对人类、植物、动物和生态系统生物学理解的这些重要研究领域，本次研讨会的资金由生理和结构系统集群、植物基因组研究提供。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Defining the commonalities between post-transcriptional and post-translational modification communities

定义转录后修饰群落和翻译后修饰群落之间的共性

• DOI: 10.1016/j.tibs.2023.09.011
• 发表时间: 2023-10
• 期刊: Trends in Biochemical Sciences
• 影响因子: 13.8
• 作者: Baumer, Zachary T.;Erber, Luke;Jolley, Elizabeth;Lawrence, Sheldon;Lin, Chuwei;Murakami, Shino;Perez, Veronica;Prall, Wil;Schaening;Sylvia, Megan;et al
• 通讯作者: et al