Collaborative Research RUI: Examination of the Effect of Templating Agents on the Size, Shape and Chiral Recognition of Bifurcated Amino Acid Based Amphiphilic Molecular Assemblies

合作研究 RUI：检查模板剂对基于分叉氨基酸的两亲性分子组装体的尺寸、形状和手性识别的影响

• 批准号: 2203506
• 负责人: Kevin Morris
• 金额: $ 8.54万
• 依托单位: Carthage College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203506&HistoricalAwards=false
• 关键词: Collaborative; Research; RUI; Examination; Effect

With the support of the Macromolecular, Supramolecular and Nanochemistry Program in the Division of Chemistry, Eugene Billiot, Fereshteh Billiot and Mark Olson of Texas A&M University Corpus Christi (TAMUCC), along with their collaborator Kevin Morris at Carthage College, will synthesize and characterize a novel class of “branched” amino acid-based surfactants. The goal of the research is to understand the molecular factors responsible for the aggregation and self-assembly of these molecules into structures that will interact with and recognize molecules of biological importance. Synthesis of the surfactants will use a green chemistry approach where materials will be prepared from amino acid-based starting materials instead of potentially hazardous polyaromatic hydrocarbons. Furthermore, since the surfactants are amino acid-based, they are expected to be biocompatible. The research will help fill a void in the current knowledge base that is critical for the proper design and selection of more efficient chiral selectors. In addition, since enantiorecognition of the systems under study is based upon amino acid-scaffolding, the knowledge gained from these studies will be potentially transferable to other amino acid-based systems such as proteins. This project is a collaboration between two primarily undergraduate institutions, with one of those two institutions, TAMUCC, being a Hispanic-serving institution (HSI). Thus, the research to be funded by this grant will serve as a tool for the research team to recruit, train, and mentor students from underrepresented and economically challenged backgrounds and to encourage these students to pursue post baccalaureate degrees in science.The research is focused on investigating and elucidating the factors responsible for (a) self-assembly, (b) function, (c) higher ordered structure and (d) molecular recognition of amino acid-based macromolecular assemblies (AABMAs). A comparison of the properties of “linear” AABMAs to novel “bifurcated” AABMAs will be made and how they are affected by factors such as surfactant concentration, surfactant type (linear vs bifurcated), pH, and type of counterion/templating agent affect the size, shape, physical properties, and enantiomeric recognition of AABMAs. The AABMAs will be studied in both their monomeric and polymeric forms. The AABMAs micelles will be crosslinked by exposure to Co60 gamma radiation or 185 nm vacuum UV radiation. The effect of pH, counterion/templating agent, and surfactant concentration on polymerization, self-assembly, function, higher order structure and molecular recognition will be studied. A variety of techniques such as nuclear magnetic resonance (NMR), fluorescence, Small Angle Neutron Scattering (SANS), small angle X-ray scattering, cryo-transmission electron microscopy, dynamic light scattering methods, as well as capillary electrophoresis (CE) will be utilized to learn more about the physical properties of the AABMAs formed. Thermodynamic studies will be conducted to yield insight into the binding interactions between the chiral molecular recognition units of the surfactants/polymer micelles and the chiral analytes that will be targeted for chiral separations using titration microcalorimetry.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系高分子、超分子和纳米化学项目的支持下，德克萨斯农工大学科珀斯克里斯蒂分校 (TAMUCC) 的 Eugene Billiot、Fereshteh Billiot 和 Mark Olson 以及迦太基学院的合作者 Kevin Morris 将合成并表征新型“支链”氨基酸表面活性剂该研究的目标是了解导致聚集和形成的分子因素。这些分子自组装成能够与具有生物重要性的分子相互作用并识别的结构，表面活性剂的合成将使用绿色化学方法，其中材料将从基于氨基酸的起始材料而不是潜在危险的多环芳烃中制备。由于表面活性剂是基于氨基酸的，因此该研究将有助于填补当前知识库的空白，这对于正确设计和选择更有效的手性选择剂至关重要。正在研究的系统基于氨基酸支架，从这些研究中获得的知识将有可能转移到其他基于氨基酸的系统，例如蛋白质。该项目是两个主要本科机构之间的合作，其中两个机构之一， TAMUCC 是一所西班牙裔服务机构 (HSI)，因此，这笔赠款资助的研究将作为研究团队招募、培训和指导来自代表性不足和经济困难背景的学生的工具，并鼓励这些学生。追求职位该研究的重点是调查和阐明导致以下因素的因素：(a) 自组装、(b) 功能、(c) 高级有序结构和 (d) 基于氨基酸的大分子组装体 (AABMA) 的分子识别将比较“线性”AABMA 与新型“分叉”AABMA 的特性，以及它们如何受到表面活性剂浓度、表面活性剂类型（线性）等因素的影响。与分叉）、pH 值和抗衡离子/模板剂类型会影响 AABMA 的尺寸、形状、物理性质和对映体识别。 AABMA 胶束将通过暴露于 AABMA 的单体和聚合形式进行交联。 Co60 伽马辐射或 185 nm 真空紫外线辐射 pH、抗衡离子/模板剂和表面活性剂浓度对聚合、自组装、功能的影响。将研究各种技术，例如核磁共振（NMR）、荧光、小角中子散射（SANS）、小角X射线散射、低温透射电子显微镜、动态光散射方法。以及毛细管电泳（CE）将用于更多地了解所形成的 AABMA 的物理性质，并将进行热力学研究，以深入了解 AABMA 的手性分子识别单元之间的结合相互作用。表面活性剂/聚合物胶束和手性分析物将成为使用滴定微量热法进行手性分离的目标。该奖项符合 NSF 的法定使命，并且通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

NMR investigation of counterion binding to undecyl LL-leucinevalanate micelles

抗衡离子与十一烷基 LL-亮氨酸戊酸胶束结合的 NMR 研究

• DOI: 10.1080/01932691.2022.2145303
• 发表时间: 2022
• 期刊: Journal of Dispersion Science and Technology
• 影响因子: 2.2
• 作者: Fletcher, Jessica;Mahant, Grant;Witzleb, Tyler;Busche, Riley;Garcia, Mauro;Fang, Yayin;Billiot, Eugene J.;Billiot, Fereshteh H.;Morris, Kevin F.
• 通讯作者: Morris, Kevin F.