NSF : EiR: Building capacity at an HCBU: Tropical plants, their endosymbionts, and their metabolomes.

NSF：EiR：HCBU 能力建设：热带植物、其内共生体及其代谢组。

• 批准号: 2200596
• 负责人: Anne Osano
• 金额: $ 50万
• 依托单位: Bowie State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2200596&HistoricalAwards=false
• 关键词: NSF; EiR; Building; capacity; HCBU

Diverse plant species are used traditionally by diverse human cultures worldwide for their medicinal properties. These plants are culturally important and impactful because chemical compounds (metabolites) from them can have therapeutic effects. It is now understood that all plants host diverse microbes within their tissues, including those used medicinally. This project focuses on two species of traditional importance in Africa to ask how fungi and bacteria occurring within plants can influence plant metabolites of medicinal use, either by increasing or changing the expression of plant metabolites, or by expressing their own chemical compounds. This project will advance an understanding of the ways in which plant-affiliated microbes influence the metabolites of plants while supporting training for undergraduates at Bowie State University (BSU), a Historically Black College and University (HBCU), in international and inter-institutional research experiences that range from field collections in Kenya to training at US partner universities in molecular ecology and the science of metabolomes (metabolomics). The observation that microbes occur within living plant tissues as endophytes raises the question: to what degree are endophytes responsible for the secondary metabolites attributed to medicinal plants by cultural practitioners and scientists alike? This project will (1) characterize endophyte communities in above-ground tissues of the medicinal plants Azadiracta indica (neem) and Melia azedarach (melea) in Kenya and the US, (2) use inoculation experiments to establish metabolite profiles for each species in the presence and absence of selected endophytes, and (3) characterize and quantify the production of metabolites by selected endophytes on diverse substrates. This project will provide insight into an understudied but ubiquitous symbiosis – that of endophytes and the host plants whose tissues humans use directly – to link plant metabolic phenotypes to those of their microbial symbionts. It will do so by engaging students from BSU in (a) authentic academic-year and classroom-based research and (b) mentored research in Kenya and at the University of Arizona (a Hispanic-Serving Institution, HSI), (c) in collaboration with a metabolomics laboratory at Morgan State University (an HBCU). By providing capacity-building and training, this project will expand opportunities in microbiology, metabolomics, plant science, and international engagement for undergraduates, support a cross-cutting international and inter-institutional research experience for BSU students, foster authentic research and support a postdoc in the plant science curriculum at BSU, and strengthen ties among US HBCUs, an HSI, and international partners in Kenya.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

传统上，不同的植物物种因其药用特性而被世界各地的不同人类文化所使用，这些植物在文化上具有重要意义和影响力，因为它们的化学化合物（代谢物）可以具有治疗作用。该项目重点关注非洲传统上重要的两种物种，以探究植物中存在的真菌和细菌如何通过增加或改变植物代谢物的表达，或通过表达来影响药用植物代谢物。该项目将促进人们对植物相关微生物影响植物代谢物的方式的了解，同时支持鲍伊州立大学 (BSU)（一所历史悠久的黑人学院和大学 (HBCU)）的本科生培训。以及机构间的研究经验，从肯尼亚的实地采集到在美国合作大学的分子生态学和代谢组学（代谢组学）培训。问题：文化从业者和科学家认为内生菌在多大程度上对药用植物的次生代谢物负责？该项目将 (1) 描述药用植物印楝 (neem) 和苦楝 (Melia azedarach) 地上组织中内生菌群落的特征。 melea）在肯尼亚和美国，（2）使用接种实验在存在和不存在选定内生菌的情况下建立每个物种的代谢物谱，以及（3）表征和量化该项目将深入了解一种尚未得到充分研究但普遍存在的共生关系——内生菌与人类直接使用其组织的宿主植物的共生关系，从而将植物代谢表型与其微生物共生体的代谢表型联系起来。让 BSU 的学生参与 (a) 真实的学年和课堂研究，以及 (b) 在肯尼亚和亚利桑那大学（西班牙裔服务机构，HSI）指导研究， (c) 与摩根州立大学（HBCU）的代谢组学实验室合作，通过提供能力建设和培训，该项目将扩大本科生在微生物学、代谢组学、植物科学和国际参与方面的机会，支持跨领域的研究。为 BSU 学生提供国际和机构间研究经验，促进真实研究并支持 BSU 植物科学课程的博士后，并加强美国 HBCU、HSI 和肯尼亚国际合作伙伴之间的联系。该奖项反映了通过使用基金会的智力价值和更广泛的影响审查标准进行评估，NSF 的法定使命被认为值得支持。