SBIR Phase I: Oral delivery systems for sterilizing strains of a sexually-transmitted insect virus

SBIR 第一阶段：用于对性传播昆虫病毒株进行灭菌的口服给药系统

• 批准号: 2126953
• 负责人: Bruce Webb
• 金额: $ 25.6万
• 依托单位: Lepidext LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2126953&HistoricalAwards=false
• 关键词: SBIR; Phase; Oral; delivery; systems

The Broader Impact of this Small Business Innovation Research (SBIR) Phase I project is to support widespread distribution and adoption of sterilizing biopesticides that are effective and efficient to control Lepidoptera. Lepidopterans, such as the corn earworm, circumvent insecticidal properties of certain crops by developing resistance. Models show that incorporating sterile insects in a corn earworm population can suppress the incidence of resistant individuals in the populations. Enhancing oral infectivity of this sterilizing insect biopesticide will improve product manufacturing, distribution and most importantly, ease of use to support farmer adoption.The proposed project develops a functional orally-infectious formula of a specific strain of the sexually-transmitted, sterilizing-virus, Helicoverpa zea nudivirus 2 (HzNV2). The resulting technology will be suitable for prototyping and testing oral formulations for sterilizing adult corn earworm moths. Genetic selection and candidate gene approaches are proposed to improve stability of the virus entry complex required for oral infectivity. This project will decorate viral envelopes with genes absent from HzNV-2 and thereby improve virion stability. The pseudotyped virus will be assayed for increased oral infectivity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该小企业创新研究 (SBIR) 第一阶段项目的更广泛影响是支持广泛分发和采用可有效且高效地控制鳞翅目的灭菌生物农药。鳞翅目昆虫，例如玉米穗虫，通过产生抗性来规避某些作物的杀虫特性。 模型表明，将不育昆虫纳入玉米穗虫种群中可以抑制种群中抗性个体的发生率。 增强这种昆虫灭菌生物农药的口腔传染性将改善产品的制造和分销，最重要的是，提高使用的便利性以支持农民采用。拟议的项目开发了一种功能性口腔感染配方，该配方是性传播灭菌病毒的特定菌株，玉米夜蛾裸病毒 2 (HzNV2)。由此产生的技术将适用于对成年玉米螟蛾进行灭菌的口服制剂的原型设计和测试。提出了遗传选择和候选基因方法来提高口腔感染性所需的病毒进入复合物的稳定性。该项目将用 HzNV-2 中缺少的基因装饰病毒包膜，从而提高病毒粒子的稳定性。 将对假型病毒进行口腔感染性增加的检测。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。