RUI: Development of New Green-Chemical Catalysts Based on Rieske Dioxygenase Scaffolds Through Directed Evolution

RUI：通过定向进化开发基于Rieske双加氧酶支架的新型绿色化学催化剂

• 批准号: 2147098
• 负责人: Jordan Froese
• 金额: $ 36.64万
• 依托单位: Ball State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2147098&HistoricalAwards=false
• 关键词: RUI; Development; New; Green; Chemical

Enzymes could reduce the environmental impact of processes. A class of enzymes found in soil bacteria enables them to thrive in petroleum-contaminated environments. Previously uncharacterized dioxygenases will be the focus of the project. Evolving these enzymes to expand the breadth of molecules on which they operate is a key objective, and they will be screened for the ability to degrade pollutants and synthesize valuable chemical intermediates. This project will also provide contemporary education and training experiences for undergraduate and master’s level students.Rieske dioxygenases have frequently been utilized in organic synthesis and bioremediation. Applications are restricted by limited substrate ranges and strict product selectivity. Directed molecular evolution will generate new Rieske dioxygenases exhibiting: 1) expanded reactivity for aromatic compounds and 2) expanded substrate range and increased reactivity in the dihydroxylation of alkyl olefins. Enzyme engineering will follow a “design-test-build-learn” cycle, wherein large Rieske dioxygenase variant libraries will be produced using both rational engineering and random mutagenesis strategies and will be screened for enhanced activity using a “substrate multiplexing” approach. Potential contributions of this research include A) new, sustainable catalysts with valuable applications in organic synthesis and bioremediation; B) the first reliable, enzymatic alternative to chemical methods for performing the asymmetric dihydroxylation of alkyl olefins; and C) development of a blueprint for the successful engineering of Rieske dioxygenases.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

酶可能会在其上进行的环境影响在有机合成和生物修复中。使用“底物多路复用”方法。二氧酶。该奖项反映了任务，并被认为是值得使用Tounlectul功能更广泛影响的审查标准的值得支持的。

项目成果

Production of novel Rieske dioxygenase metabolites enabled by enzyme engineering

通过酶工程生产新型 Rieske 双加氧酶代谢物

• DOI: 10.1039/d3cy00262d
• 发表时间: 2023
• 期刊: Catalysis Science & Technology
• 影响因子: 5
• 作者: Osifalujo, Elizabeth A.;Rutkowski, Bailey N.;Satterwhite, Louis R.;Betts, Phillip C.;Nkosi, Angel K.;Froese, Jordan T.
• 通讯作者: Froese, Jordan T.