CAREER: The contributions of imperfect transmission and cytoplasmic incompatibility to Wolbachia frequency variation
职业生涯:不完美的传输和细胞质不相容性对沃尔巴克氏体频率变异的影响
基本信息
- 批准号:2145195
- 负责人:
- 金额:$ 149.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-06-01 至 2027-05-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Over a billion years ago a host cell engulfed a free-living bacterium in a remarkable event that led to the evolution of mitochondria that now provide the chemical energy for animal, plant, and fungal cells. Recent research has revealed that the process of microbes taking up residence inside the cells of other species (known as endosymbiosis) is very common, especially among insects. For example, a single microbe called Wolbachia infects more than half of all insect species on Earth. Unlike mitochondria, Wolbachia are usually not required for their hosts to survive and reproduce. Instead, the proportion of individuals within a host species with Wolbachia at any time varies greatly for unknown reasons, making it hard to predict the consequences for host biology. This project will unravel the causes of variable Wolbachia prevalence as a model for more broadly understanding conditions that favor endosymbiosis. This work will also potentially contribute to improving human health. Wolbachia can block viruses like dengue and Zika in mosquito systems, but increasing the efficacy of this work requires identifying conditions that favor high Wolbachia prevalence. This project will identify such conditions in natural systems. Locally, this project will educate and train first-generation and Native Montana students in cutting-edge laboratory, field, and computational approaches that will improve their competitiveness in STEM-related careers. A new course at the University of Montana focused on host-microbe interactions will be further developed to include outreach activities aimed at exposing individuals in rural Montana to the unique biology of endosymbiosis. Organismal performance and fitness depends on interactions across levels of biological organization. Among all species interactions, those between animals and microbial endosymbionts that live inside their cells are perhaps the most intimate, influencing host behavior, physiology, life history, and fitness. Some endosymbionts may even contribute to reproductive isolation and speciation, highlighting their broad importance for host biology. The evolutionary outcomes of host-endosymbiont interactions ultimately depend on endosymbiont prevalence in host populations, which varies widely for unknown reasons. This project will leverage 50 million years of Wolbachia evolution distributed across divergent host species to determine the causes of Wolbachia frequency variation. The project will specifically dissect how genes in host and endosymbiont genomes interact with each other and with temperature (GxGxE interactions) to influence maternal Wolbachia transmission rates and the strength of Wolbachia-induced reproductive manipulations like cytoplasmic incompatibility. Mathematical models will then be used to evaluate how both genomic and environmental interactions contribute to Wolbachia prevalence in divergent host systems. At each step, this project includes activities that will leverage the unique biology of endosymbiosis to educate and train first-generation and Native students in Montana.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
十亿多年前,宿主细胞在一次非凡的事件中吞噬了一种自由生活的细菌,这导致了线粒体的进化,现在线粒体为动物、植物和真菌细胞提供化学能。最近的研究表明,微生物在其他物种的细胞内定居的过程(称为内共生)非常常见,尤其是在昆虫中。例如,一种名为沃尔巴克氏体的微生物感染了地球上一半以上的昆虫物种。与线粒体不同,沃尔巴克氏体通常不需要宿主的生存和繁殖。相反,在任何时候,宿主物种中携带沃尔巴克氏体的个体比例由于未知原因而变化很大,因此很难预测对宿主生物学的影响。该项目将揭示沃尔巴克氏体患病率变化的原因,作为更广泛地理解有利于内共生的条件的模型。这项工作也可能有助于改善人类健康。沃尔巴克氏体可以阻止蚊子系统中的登革热和寨卡病毒等病毒,但提高这项工作的功效需要确定有利于沃尔巴克氏体高流行的条件。该项目将识别自然系统中的此类条件。在当地,该项目将利用尖端的实验室、现场和计算方法对第一代和蒙大拿州本地学生进行教育和培训,以提高他们在 STEM 相关职业中的竞争力。蒙大拿大学将进一步开发一门专注于宿主与微生物相互作用的新课程,其中包括旨在让蒙大拿州农村地区的个体接触内共生的独特生物学的外展活动。有机体的性能和适应性取决于生物组织各个层面的相互作用。在所有物种相互作用中,动物与其细胞内的微生物内共生体之间的相互作用可能是最密切的,影响着宿主的行为、生理学、生活史和健康。一些内共生体甚至可能有助于生殖隔离和物种形成,突出了它们对宿主生物学的广泛重要性。宿主与内共生体相互作用的进化结果最终取决于宿主群体中内共生体的流行程度,但由于未知的原因,内共生体的流行程度差异很大。该项目将利用分布在不同宿主物种中 5000 万年的沃尔巴克氏体进化来确定沃尔巴克氏体频率变化的原因。该项目将专门剖析宿主和内共生体基因组中的基因如何相互作用以及与温度相互作用(GxGxE相互作用),以影响母体沃尔巴克氏体传播率和沃尔巴克氏体诱导的生殖操作(如细胞质不相容性)的强度。然后,数学模型将用于评估基因组和环境相互作用如何影响不同宿主系统中沃尔巴克氏体的流行。该项目的每一步都包括利用独特的内共生生物学来教育和培训蒙大拿州第一代和原住民学生的活动。该奖项反映了 NSF 的法定使命,并通过使用基金会的智力优点和能力进行评估,被认为值得支持。更广泛的影响审查标准。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Brandon Cooper其他文献
A Flexible Professional Development Program for International Instructors and Its Suitability for a Brazilian Context
灵活的国际教练专业发展计划及其对巴西国情的适用性
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
Ginessa Payne;Brandon Cooper;Xueyan Hu;Bruno Figueiredo;Gardenya Felix - 通讯作者:
Gardenya Felix
Brandon Cooper的其他文献
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