Collaborative Research: Does Cytonuclear Coevolution Drive Reproductive Isolation? Dissecting the Architecture of Genetic Incompatibility Across a Species Range

合作研究：细胞核协同进化是否会导致生殖隔离？

• 批准号: 2140189
• 负责人: Laura Galloway
• 金额: $ 41.68万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2140189&HistoricalAwards=false
• 关键词: Collaborative; Research; Does; Cytonuclear; Coevolution

The natural world exhibits a vast array of biodiversity which ensures the stability of ecosystems, the services they provide, and has intrinsic aesthetic value. Individual species are critical elements of this biodiversity and understanding how new species form is key to ensuring maintenance of diversity. Interactions between the nuclear genome and organelle (mitochondria and chloroplast) genomes underlie key processes including respiration and photosynthesis. These interactions are an underappreciated and yet potentially powerful driver of the earliest stages of speciation. The planned research will investigate the contribution of intergenomic interactions to incompatibility between populations of a species. The research will use state-of-the art techniques to identify the genomic basis of incompatibility between the nuclear and organelle genomes and determine the ability of such incompatibility to maintain genetic divergence and facilitate speciation in a contact zone. The project will also introduce undergraduate students and high-school and community college teachers to core ideas about species formation and evolution through hands-on experience with the newest genomic sequencing technologies in a novel course-based lab module and workshop. Finally, the project will provide training at the undergraduate, graduate, and post-graduate level.Interactions between cytoplasmic and nuclear gene products are expected to drive intergenomic coevolution, leading to the potential for genetic incompatibility and reproductive isolation between populations with divergent cytoplasmic genomes. While such cytonuclear incompatibility (CNI) has been posited to be among the earliest reproductive barriers to develop during speciation, our understanding of the dynamics of CNI at the early stages of speciation remains limited, particularly for plastid-driven speciation. The research will examine the dynamics of plastid driven CNI in Campanula americana, creating a powerful set of genomic resources, characterizing variation in the genetic architecture of CNI across lineages, and leveraging a natural contact zone to evaluate whether exposure to selection alters the genetic architecture of CNI relative to allopatry. Together, these data will provide insight into the evolutionary dynamics of plastid-nuclear incompatibility and how these dynamics may drive the early stages of speciation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

自然世界展示了各种各样的生物多样性，可确保生态系统的稳定性，他们提供的服务并具有内在的美学价值。单个物种是这种生物多样性的关键要素，并了解新物种形成如何确保维持多样性的关键。核基因组与细胞器（线粒体和叶绿体）基因组之间的相互作用是关键过程，包括呼吸和光合作用。这些相互作用是最早的物种阶段的被低估但潜在的强大驱动力。计划的研究将研究基因组相互作用对物种种群之间不兼容的贡献。该研究将使用最先进的技术来确定核和细胞器基因组之间不相容性的基因组基础，并确定这种不相容性保持遗传差异并促进接触区中物种形成的能力。该项目还将通过与最新的基于课程的实验室模块和工作室中最新的基因组测序技术进行动手实践经验，将本科生以及高中和社区大学教师介绍有关物种形成和进化的核心思想。最后，该项目将在本科，研究生和研究生级别提供培训。预计细胞质和核基因产物之间的相互作用有望促进基因组间协同进化，从而导致遗传性不相容性和种群与分子细胞质基因组之间的遗传性不相容和生殖隔离。尽管已经将这种细胞核不相兼容（CNI）被认为是物种形成期间发展的最早的生殖障碍之一，但我们对物种早期CNI动态的理解仍然有限，特别是对于质体驱动的物种。该研究将研究Campanula Americana中质体驱动的CNI的动态，创造了一组强大的基因组资源，表征了CNI跨谱系的遗传结构的变化，并利用自然接触区来评估接触选择是否会改变选择相对于Allopatry的CNI基因建筑。这些数据将共同洞悉质体 - 核不相容性的进化动力学，以及这些动态如何推动物种物种的早期阶段。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的评估标准通过评估来支持的。