Investigating the Molecular, Neurophysiological and Behavioral Effects of Hypoxia in Marine Mollusks

研究缺氧对海洋软体动物的分子、神经生理和行为影响

基本信息

批准号：
2112265
负责人：
Javier Rodriguez-Casariego
金额：
$ 25万
依托单位：
Rodriguez-Casariego, Javier A
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2112265&HistoricalAwards=false
关键词：
Investigating Molecular Neurophysiological Behavioral Effects

项目摘要

The Centers of Research Excellence in Science and Technology-Postdoctoral Research Fellowship (CREST-PRF) track within the CREST program supports beginning CREST Center investigators with significant potential and provides them with training and research experiences that will broaden perspectives, facilitate interdisciplinary interactions and establish them in positions of leadership within the scientific community. This CREST-PRF project is aligned with the research focus of the CREST Center for Environmental Neuroscience at the University of Puerto Rico. The goal of this research is for the post doctoral fellow to receive training in animal neurophysiology and developmental biology. The proposed research will build on the fellow's background in epigenetics, molecular biology, and ecotoxicology. The postdoctoral fellow intends to engage with the public to promote conservation efforts focused on coastal urban ecosystems, broaden the participation of groups underrepresented in biology through active mentoring and teaching, and promote scientific collaboration between Puerto Rican universities and minority serving institutions in South Florida.Accumulating evidence suggests that epigenetic mechanisms, which are influenced by developmental and environmental signals, play a crucial role in regulating gene expression and potentially modulate behavior and memory. However, it is unclear how environmental-exposure-driven epigenetic modifications could affect short and long-term behavioral responses. The intent of the proposed work is to disentangle epigenetic effects in the establishment and plasticity of behavioral responses to stress. The proposed work will investigate linkages between molecular, cellular, and behavioral responses to hypoxia; evaluate the inheritance of molecular and narrow plasticity to stressors and the resulting behavior outcomes; and studying these plastic responses in coastal urban environments with a recent history of hypoxic events. Neurohistology methods, including immunohistochemistry and dye injection are expected to enable the postdoctoral fellow to work on individual identified neurons. Coupled with his prior training, the post-doctoral fellow has the potential to be uniquely positioned to embark on an independent career studying epigenetic impacts of environmental perturbations on neurological function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

CREST 计划中的科学技术卓越研究中心 - 博士后研究奖学金 (CREST-PRF) 轨道为具有巨大潜力的 CREST 中心研究人员提供支持，并为他们提供培训和研究经验，从而拓宽视野、促进跨学科互动并建立跨学科互动在科学界担任领导职务。该 CREST-PRF 项目与波多黎各大学 CREST 环境神经科学中心的研究重点一致。这项研究的目标是让博士后接受动物神经生理学和发育生物学的培训。拟议的研究将建立在该研究员的表观遗传学、分子生物学和生态毒理学背景的基础上。这位博士后研究员打算与公众合作，促进以沿海城市生态系统为重点的保护工作，通过积极的指导和教学扩大生物学领域代表性不足的群体的参与，并促进波多黎各大学与南佛罗里达州少数族裔服务机构之间的科学合作。有证据表明，受发育和环境信号影响的表观遗传机制在调节基因表达并可能调节行为和记忆方面发挥着至关重要的作用。然而，目前尚不清楚环境暴露驱动的表观遗传修饰如何影响短期和长期行为反应。拟议工作的目的是理清对压力的行为反应的建立和可塑性中的表观遗传效应。拟议的工作将研究对缺氧的分子、细胞和行为反应之间的联系；评估压力源的分子和狭义可塑性的遗传以及由此产生的行为结果；并研究具有近期缺氧事件历史的沿海城市环境中的这些塑料反应。神经组织学方法，包括免疫组织化学和染料注射，预计将使博士后研究员能够研究单个已识别的神经元。加上他之前的培训，这位博士后研究员有潜力以独特的优势开始独立的职业生涯，研究环境扰动对神经功能的表观遗传影响。该奖项反映了 NSF 的法定使命，并通过使用评估方法进行评估，认为值得支持。基金会的智力价值和更广泛的影响审查标准。