Collaborative Research: ERASE-PFAS: Remediation of Per- and Polyfluoroalkyl Substances in Wastewater using Anaerobic Membrane Bioreactors

合作研究：ERASE-PFAS：利用厌氧膜生物反应器修复废水中的全氟烷基和多氟烷基物质

基本信息

批准号：
2112201
负责人：
Diana Aga
金额：
$ 35万
依托单位：
SUNY at Buffalo
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2112201&HistoricalAwards=false
关键词：
Collaborative Research ERASE PFAS Remediation

项目摘要

Per- and polyfluoroalkyl substances (PFASs) are used in many consumer and industrial products. PFASs have been found in domestic drinking water systems and have been identified in ecosystems on a global basis. PFASs are highly persistent in the environment, and as such have been called ‘forever chemicals.’ Because PFASs are toxic to humans and wildlife, it is important to find efficient ways to eliminate these chemicals from water supplies. The goal of this research is to address this need through the development of anaerobic membrane bioreactors (AnMBRs) that rely on both bacteria and membranes to remove and destroy PFASs from water. This goal will be achieved through a multiphase research program to develop microbial cultures that transform PFAS using novel molecular biological approaches, characterize the PFAS transformation process before and after treatment, and assess the reactivity of end products using state-of-the-science approaches. Successful completion of this research will allow us to better understand how bacteria degrade PFASs and determine how degradation affects the toxicity of these products. Societal benefits include potential development of technology to address the urgent national need for low cost, effective PFAS treatment. Additional benefits include increasing scientific literacy and STEM diversity through outreach, recruitment, and training.The widespread use and extreme stability of PFASs have resulted in their ubiquitous occurrence in the environment. The recalcitrance of PFASs to biodegradation resulting from the inertness of carbon-fluorine bonds is poorly understood. However, reductive defluorination is thermodynamically favorable under reducing conditions, a puzzling finding that warrants further exploration using emerging chemical and molecular biotechnological tools. This project addresses this need through a multi-stage investigation of the biodegradation of PFASs in AnMBRs. AnMBRs combine anaerobic treatment with membrane separation, providing low-energy intensive biological treatment. The overall goal of this project is to develop a set of tools leading to a better understanding of PFAS biotreatment. The specific objectives designed to achieve this goal are to: i) demonstrate reductive defluorination of PFASs in AnMBRs and identify defluorinating microbial populations using emulsion, paired isolation, and concatenation (epic)PCR to link phylogenetic genes with dehalogenation genes at a cellular level; ii) characterize biotransformation products and assess degradation efficiency using high resolution liquid chromatography/mass spectrometry and 19F nuclear magnetic resonance spectroscopy; and iii) systematically evaluate biological activity of PFAS mixtures in mammalian cell lines using an integrated transcriptomics and metabolomics approach. Successful completion of this research holds strong potential to transform our knowledge of water treatment systems for legacy and emerging PFASs. Such information can lead to efficient biological treatment alternatives for PFAS, addressing a critical national need. Broader scientific and societal impacts include the potential for a paradigm shift in current practices for establishing PFAS health advisories should evidence of synergistic interactions in complex PFAS mixtures be established.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

许多消费者和工业产品都使用了多氟烷基物质（PFAS）。在国内饮用水系统中发现了PFAS，并在全球生态系统中被发现。 PFAS在环境中高度持久，因此称为“永远的化学物质”。由于PFAS对人类和野生动植物有毒，因此找到有效的方法来消除这些化学物质从供水中消除这些化学物质非常重要。这项研究的目的是通过厌氧膜生物反应器（ANMBRS）的发展来满足这种需求，这些生物反应器（ANMBRS）依靠细菌和膜来清除和破坏水中的PFASS。该目标将通过多相研究计划实现，以开发使用新型分子生物学方法转化PFA的微生物培养物，表征治疗前后的PFAS转化过程，并使用先知方法评估最终产品的反应性。成功完成这项研究将使我们能够更好地了解细菌如何降解PFASS并确定降解如何影响这些产品的毒性。社会利益包括潜在的技术发展，以应对国家对低成本，有效PFA治疗的紧迫需求。其他好处包括通过外展，招聘和培训来提高科学素养和茎多样性。PFASS的宽度使用和极端稳定性导致环境中无处不在。 PFASS对生物降解的顽固性因碳氟键的惰性而引起的生物降解尚不清楚。然而，在还原条件下，减少的屈光度在热力学上是有利的，这是一个难题发现，需要使用新兴的化学和分子生物技术工具进一步探索。该项目通过对ANMBRS中PFAS的生物降解的多阶段研究来满足这一需求。 ANMBRS结合厌氧处理与膜分离，提供低能强化生物学处理。该项目的总体目标是开发一套工具，从而更好地了解PFAS生物治疗。旨在实现此目标的特定目标是：i）证明使用乳液，成对的隔离和串联（EPIC）PCR将PFASS降低降低，并鉴定微生物种群，以将系统理性基因与细胞水平的脱闭基因联系起来； ii）使用高分辨率液相色谱/质谱法和19F核磁共振光谱法表征生物转化产物和评估降解效率； iii）使用集成转录组学和代谢组学方法系统地评估哺乳动物细胞系中PFA的混合物的生物学活性。这项研究的成功完成具有强大的潜力，可以改变我们对遗产和新兴PFAS的水处理系统的了解。此类信息可以为PFA提供有效的生物治疗替代方法，从而满足至关重要的国家需求。更广泛的科学和社会影响包括在当前实践中建立PFAS健康咨询的范式转变的潜力，应建立复杂PFAS组合中的协同互动的证据。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识和更广泛的影响来审查Criteria来通过评估来通过评估来获得支持的珍贵的。