Collaborative: BEE: C-EVO: Linking Carbon cycling to eco-EVOlutionary responses of a foundational plant to global change

合作：BEE：C-EVO：将碳循环与生态进化联系起来基础植物对全球变化的反应

• 批准号: 2051598
• 负责人: Michael Blum
• 金额: $ 49.76万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2051598&HistoricalAwards=false
• 关键词: Collaborative; BEE; EVO; Linking; Carbon

There is a growing appreciation for how evolutionary responses to environmental change can alter vital ecosystem attributes. This project aims to study how evolutionary responses of an ecologically dominant plant to elevated atmospheric carbon dioxide concentration and nitrogen enrichment influence the flow of carbon into and out of coastal marsh ecosystems. The researchers plan to leverage a long-term experiment to characterize how plant traits that impact photosynthesis, the decomposition of organic matter in marsh sediments, and sediment accretion shift in response to increased carbon dioxide and nitrogen availability. They then plan to establish the underlying genetic basis for these traits, so that changes in genetic variation can be used to confirm that evolution has taken place. And finally, the researchers plan to quantify the consequences of changes in traits, and their associated genetic variation, for long-term carbon storage in, or loss from, highly productive marsh ecosystems. This would enable the researchers to identify mechanistic and functional links between plant evolution and carbon cycling in coastal marshes, which face an uncertain future due to anticipated sea-level rise. Findings from this research have the potential to improve our understanding of the global carbon budget, and to foster more effective coastal restoration by advancing our understanding of marsh persistence. Furthermore, the project can strengthen research infrastructure, create new educational and outreach opportunities, including the engagement of citizen scientists, and facilitate undergraduate research by students from groups underrepresented in science.This project employs a complementary set of studies explicitly designed to bridge evolutionary biology and ecosystem ecology. Using a combination of new experiments and tissue archives from an ongoing, long-term global change experiment, the researchers plan to simultaneously assay in situ genetic and genomic variation using ddRAD-generated SNPs, to characterize functional trait (e.g. maximum rates of photosynthesis, stomatal conductance, and water use efficiency) variation in the field, and to quantify complete marsh carbon budgets (e.g. quantifying NPP, gaseous C losses, litter decomposition rate, and belowground biomass and C accumulation). These measurements are set in the context of more than a decade of exposure to elevated CO2 and nitrogen enrichment. To go beyond establishing associations between traits, genetic variation, and carbon flux post hoc, the researchers plan to conduct a complementary de novo quantitative genetics study of trait variation and physiology, with the intent of linking both to carbon fluxes mediated by individual plants. Establishing such mechanistic links can improve parameterization of a mechanistic model of C cycling in coastal marshes, enabling a more thorough characterization of ecosystem-level consequences of organismal evolution. Findings from this project can illustrate whether organismal evolution is an important determinant of how ecosystems function and offer new insights about how global change can directly and indirectly alter ecosystems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

人们对环境变化的进化响应如何改变重要生态系统属性的越来越多。该项目旨在研究生态上占主导地位的植物对升高大气二氧化碳浓度和氮富集的进化反应如何影响碳进入沿海沼泽生态系统的流动。 研究人员计划利用长期实验来表征如何影响光合作用的植物特征，沼泽沉积物中有机物的分解以及沉积物积聚，以响应增加二氧化碳和氮的可用性。 然后，他们计划建立这些特征的基本遗传基础，以便可以使用遗传变异的变化来确认发生了进化。 最后，研究人员计划量化性状变化的后果及其相关的遗传变异，以使其长期碳储存或损失高生产力的沼泽生态系统。 这将使研究人员能够确定沿海沼泽中植物进化与碳循环之间的机械和功能联系，由于预期的海平面上升，这面临着不确定的未来。这项研究的发现有可能提高我们对全球碳预算的理解，并通过促进我们对沼泽持久性的理解来促进更有效的沿海恢复。 此外，该项目可以加强研究基础设施，创造新的教育和推广机会，包括公民科学家的参与，并促进来自科学领域中代表性不足的学生的本科研究。该项目采用了一套互补的研究集，该研究明确设计，旨在弥合进化生物学和生态系统生态学。研究人员使用持续的长期全球变化实验的新实验和组织档案的结合，计划使用DDRAD生成的SNP同时测定原位遗传和基因组变异，以表征功能性状（例如，最大的光合作用速率气体C损失，垃圾分解速率以及地下生物量和C积累）。 这些测量值是在二十多年的二氧化碳和氮富集的情况下设置的。为了超越建立性状，遗传变异和碳通量之间的关联，研究人员计划对性状变异和生理学进行从头定量遗传学研究进行互补，并将两者都与由单个工厂介导的碳通量联系起来。建立这种机械联系可以改善沿海沼泽中C骑自行车的机械模型的参数化，从而更彻底地表征了生态系统层面的后果。该项目的发现可以说明有机化的进化是否是生态系统如何运作的重要决定因素，并提供有关全球变化如何直接和间接改变生态系统的新见解。该奖项反映了NSF的法定任务，并认为通过使用该基金会的知识分子和更广泛的影响来评估Criteria的评估，并被视为值得通过评估的支持。