CAREER:Omic Insights into Stony Coral Tissue Loss Disease

职业：Omic 对石质珊瑚组织损失病的见解

• 批准号: 2047235
• 负责人: Neha Garg
• 金额: $ 89.67万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2026-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2047235&HistoricalAwards=false
• 关键词: CAREER; Omic; Insights; into; Stony

The CAREER award will examine the changing chemical and microbial make up of coral reefs experiencing outbreaks of stony coral tissue loss disease (SCTLD). Coral reefs support diverse ocean life and sustain economic growth in coastal areas. Coral reefs are imperiled by disease outbreaks, climate change, and anthropogenic activities. Specifically, SCTLD is spreading unabated in Florida and the Caribbean and has infected over 20 coral species. Despite this spread, the understanding of chemical changes that accompany the onset of disease, the changes in bacterial inhabitants, and the disruption of the coral-algal symbiosis, remains poorly known. The principal investigator will elucidate the chemical changes that occur during SCTLD progression in corals using multi-omics and data analytic strategies. The bacteria and algae that may be responsible for these chemical changes will be identified. Knowledge gained from this research will provide understanding of the chemical and organismal signatures that define health or disease status of corals. To enhance the reach of this research, teaching modules will be created for high school, undergraduate and graduate students and K-12 teachers. Introduction of high school students to the effects of climate change on coral reefs and role of STEM in understanding disease mechanisms may enhance student participation in STEM fields and serve as a strong scientific foundation for these students. Very little is known about pathogenic mechanisms in marine environments. Unabated spread of SCTLD provides a unique opportunity to understand pathogenesis in the marine environment. The specific goal of this project is to decipher the role of algal and microbial symbionts in the health of coral holobionts at the chemical level via the implementation of multi-omic strategies including metabolomics of field corals, cultured microbial and algal symbionts, and microbiome sequencing. This research will provide fundamental insights into 1) natural product metabolites that co-occur with pathogenic microbial consortia, 2) natural products that are produced by the microbiome and the endosymbionts prior to onset of disease and during recovery post-treatment, and 3) how changes in the microbiome during disease are linked to changes in the chemical makeup of corals across different species. The paired metabolomics and 16S amplicon sequencing data generated in this proposal is the first step towards building future efforts in integrating metagenomics, transcriptomics, proteomics, and metabolomics data to understand molecular markers underlying SCTLD and to delineate biochemical pathways underlying coral host-microbe, host-algal symbiont, and host-pathogen relationships. By identifying chemical signatures of the disease state, this research may result in new strategies to mitigate SCTLD.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

职业奖将检查珊瑚礁的变化化学和微生物组成，这些珊瑚礁经历了石质珊瑚组织损失疾病（SCTLD）的爆发。珊瑚礁支持多种海洋生物，并维持沿海地区的经济增长。珊瑚礁受到疾病爆发，气候变化和人为活动的影响。具体而言，SCTLD在佛罗里达州和加勒比海地区不断扩大，并感染了20多种珊瑚物种。 尽管传播了这种扩散，但对疾病发作，细菌居民的变化以及珊瑚 - 阿尔加尔共生的破坏的理解仍然鲜为人知。首席研究者将使用多词和数据分析策略阐明珊瑚中SCTLD进展过程中发生的化学变化。将确定可能导致这些化学变化的细菌和藻类。从这项研究中获得的知识将提供对定义珊瑚健康或疾病状况的化学和有机体特征的理解。为了提高这项研究的影响力，将为高中，本科和研究生以及K-12老师创建教学模块。引入高中生，使气候变化对珊瑚礁的影响以及STEM在理解疾病机制中的作用可能会增强学生在STEM领域的参与，并为这些学生提供强大的科学基础。 关于海洋环境中的致病机制知之甚少。 SCTLD的不断扩大为了解海洋环境中的发病机理提供了独特的机会。该项目的具体目标是通过实施包括田间珊瑚的代谢组学，培养的微生物和藻类对称体的代谢组学，以及化学水平上的藻类和微生物共生体在化学级别的健康中的作用。这项研究将为1）与致病性微生物群合相关的天然产物代谢产物的基本见解，2）由微生物组和疾病发作之前和恢复后恢复后产生的天然产物，这些天然产物以及在恢复后恢复期间以及3）在疾病期间与微生物组的变化与化学物质的变化相互不同的物种，而corals跨越了corals corals corals corals corals corals of Corals of Corals of Corals of Corals of corals a的变化。 The paired metabolomics and 16S amplicon sequencing data generated in this proposal is the first step towards building future efforts in integrating metagenomics, transcriptomics, proteomics, and metabolomics data to understand molecular markers underlying SCTLD and to delineate biochemical pathways underlying coral host-microbe, host-algal symbiont, and host-pathogen relationships.通过确定疾病状态的化学特征，这项研究可能会导致减轻SCTLD的新策略。该奖项反映了NSF的法定任务，并且使用基金会的知识分子优点和更广泛的影响审查标准，被认为是值得通过评估来支持的。

项目成果

Metabolomics of Healthy and Stony Coral Tissue Loss Disease Affected Montastraea cavernosa Corals

受蒙塔斯特雷亚海绵体珊瑚影响的健康和石质珊瑚组织损失病的代谢组学

• DOI: 10.3389/fmars.2021.714778
• 发表时间: 2021
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: Deutsch, Jessica M.;Jaiyesimi, Olakunle A.;Pitts, Kelly A.;Houk, Jay;Ushijima, Blake;Walker, Brian K.;Paul, Valerie J.;Garg, Neha
• 通讯作者: Garg, Neha

Metabolomics Approaches to Dereplicate Natural Products from Coral-Derived Bioactive Bacteria

• DOI: 10.1021/acs.jnatprod.1c01110
• 发表时间: 2022-02-03
• 期刊: JOURNAL OF NATURAL PRODUCTS
• 影响因子: 5.1
• 作者: Deutsch, Jessica M.;Mandelare-Ruiz, Paige;Garg, Neha
• 通讯作者: Garg, Neha

Artificial intelligence for natural product drug discovery

• DOI: 10.1038/s41573-023-00774-7
• 发表时间: 2023-09-11
• 期刊: NATURE REVIEWS DRUG DISCOVERY
• 影响因子: 120.1
• 作者: Mullowney，Michael W.;Duncan，Katherine R.;Medema，Marnix H.
• 通讯作者: Medema，Marnix H.

Stony coral tissue loss disease: a review of emergence, impacts, etiology, diagnostics, and intervention

• DOI: 10.3389/fmars.2023.1321271
• 发表时间: 2024-01-25
• 期刊: FRONTIERS IN MARINE SCIENCE
• 影响因子: 3.7
• 作者: Papke，Erin;Carreiro，Ashley;Ushijima，Blake
• 通讯作者: Ushijima，Blake