Collaborative Research: RUI: OCE-BO: Biogeochemistry of diurnal vertical migration in microbial mats of Lake Huron’s sinkholes.

合作研究：RUI：OCE-BO：休伦湖污水坑微生物垫中昼夜垂直迁移的生物地球化学。

基本信息

批准号：
2046958
负责人：
Bopaiah Biddanda
金额：
$ 18.99万
依托单位：
Grand Valley State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-15 至 2024-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2046958&HistoricalAwards=false
关键词：
Collaborative Research RUI OCE BO

项目摘要

Modern-day microbial mats living on the bottom of sinkholes underneath Lake Huron experience an oxygen-poor, sulfur-rich environment resembling life on early Earth. These mat worlds are dominated by motile filaments of microbes that variably use sunlight and chemicals in their daily routines and offer opportunities for discovering novel microorganisms and ecosystem processes. Recently, complex patterns of daily vertical migration has been observed in the field, suggesting different microbes migrate vertically to the surface of the mat during daylight and nighttime. This project is unraveling the who, why and how of daily microbial migration through integration of microscopy, cultures, molecular approaches, and process rate measurements in response to changing gradients of light, sulfide and oxygen over the day-night cycle. This project places the vertical migration of microbial mats into a broader geobiological context through comparisons with other globally distributed cyanobacterial mat systems such as terrestrial springs and ice-covered Antarctic lakes. Furthermore, the diverse and versatile sinkhole mats may serve as a useful working model for robotic exploration of similar life in extraterrestrial waters like that of Jupiter’s Europa or Saturn’s Enceladus. This project is generating compelling student projects, attracting public imagination, and fueling active collaboration between two predominantly undergraduate institutions and a National Marine Sanctuary.The functioning of cyanobacteria under sulfidic, low O2-conditions is a major gap in our understanding of Earth’s oxygenation in the past. Recently, time-lapse images of diel vertical migration (DVM) were collected revealing alternating waves of vertically migrating photosynthetic and chemosynthetic filaments that followed daily fluctuating light in microbial mats in Lake Huron’s sinkholes; observations corroborated with intact mats under simulated day-night conditions in the laboratory. Such synchronized diel movement, might have played a critical role in optimizing photosynthesis, chemosynthesis, carbon burial, and oxygenation during the Precambrian. This project is evaluating the taxa involved in DVM and is probing geobiological controls on DVM under low-O2, sulfidic conditions using macro- and microscopic imaging, physico-chemical microprofiling, culturing, genetics, and allelopathic studies. Three central issues are being addressed: (1) what taxa are responsible for the DVM? (2) how and why do they perform DVM? and (3) what are the ecosystem consequences of DVM community and activity synergies? The project is revealing specific microbial populations, metabolic pathways, and geochemical processes that underpin mat biogeochemistry over the diel cycle. Studying microbial communities that have regular and measurable daily rhythms in processes that can also be tracked at micrometer scales yields an unprecedented view of the molecular underpinnings of microbial mat biogeochemistry and lays the foundation for future studies aimed at re-defining the role of autotrophic communities in ancient seas and modern ecosystems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

现代的微生物垫子生活在休伦湖下面的污水坑底部，经历了贫血富裕的环境，在地球早期重新融合了生活。这些垫子世界以微生物的图案为主，它们在日常的日常工作中多样地使用阳光和化学物质，并为发现新型微生物和生态系统过程提供了机会。最近，在田间观察到了每日垂直迁移的复杂模式，这表明不同的微生物在白天和夜间垂直迁移到垫子的表面。该项目正在通过整合显微镜，培养物，分子方法和过程速率测量，以探讨WHO，原因和方式，以响应在昼夜周期内光，硫化物和氧气的变化梯度，以响应变化的梯度。该项目通过与其他全球分布的蓝细菌垫系统（如陆地弹簧和冰覆盖的南极湖泊）进行比较，将微生物垫的垂直迁移到更广泛的地球生物环境中。此外，潜水员和多才多艺的污水坑垫可能是对诸如木星的欧罗巴（Europa）或土星的土卫（Ecceladus）等外星水域中类似生命的机器人探索的有用工作模型。该项目正在产生引人注目的学生项目，吸引公众的想象力，并加剧了两个主要是本科机构和国家海洋庇护所之间的积极合作。在硫化，低O2条件下的蓝细菌在我们过去对地球氧合的理解方面的主要差距是我们对地球氧合的理解的主要差距。最近，收集了垂直迁移（DVM）的延时图像，揭示了垂直迁移的光合作用和化学合成丝的替代波，随后每天在休伦湖污水池中的微生物垫中每天波动的光波动；在实验室的模拟昼夜条件下，观察结果证实了完整的垫子。这种同步的DIEL运动可能在优化前寒武纪期间优化光合作用，化学合成，碳埋葬和氧合方面发挥了关键作用。该项目正在评估DVM中涉及的分类单元，并使用宏观成像，物理化学的微型培养，培养，遗传学和化属性研究探测DVM对DVM的地球学控制。正在解决三个中心问题：（1）哪些分类单元负责DVM？（2）他们如何以及为什么执行DVM？（3）DVM社区和活动协同作用的生态系统后果是什么？该项目正在揭示特定的微生物种群，代谢途径和地球化学过程，这些过程是在DIEL周期内基础生物地球化学的基础。研究微生物群落在过程中具有定期和可测量的每日节奏，也可以在微尺尺度上跟踪，这对微生物MAT生物地球化学的分子基础的前所未有的观点产生了前所未有的观点，并为未来的研究奠定了基础，目的是重新定义自动化环境统计范围内的自动化状态，并为未来的研究奠定了基础。使用基金会的智力优点和更广泛的影响标准，认为通过评估被认为是宝贵的支持。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Extant mat microbes synchronize vertical migration to a diel tempo

DOI：
10.1016/j.jglr.2022.10.006
发表时间：
2023-02-01
期刊：
JOURNAL OF GREAT LAKES RESEARCH
影响因子：
2.2
作者：
Biddanda，Bopaiah A.;Weinke，Anthony D.;Stone，Ian P.
通讯作者：
Stone，Ian P.

Unbiased analyses of ITS folding motifs in a taxonomically confusing lineage: Anagnostidinema visiae sp. nov. ( cyanobacteria )

对分类学上令人困惑的谱系中 ITS 折叠基序的公正分析：Anagnostidinema visiae sp。

DOI：
10.1111/jpy.13337
发表时间：
2023
期刊：
Journal of Phycology
影响因子：
2.9
作者：
McGovern, Callahan A.;Norwich, Alyson R.;Thomas, Aimee L.;Hamsher, Sarah E.;Biddanda, Bopaiah A.;Weinke, Anthony D.;Casamatta, Dale A.
通讯作者：
Casamatta, Dale A.

Modern Microbial Mats Offer Glimpses of Other Times and Places

现代微生物垫提供了其他时间和地点的一瞥

DOI：
10.1029/2024eo240019
发表时间：
2024
期刊：
Eos
影响因子：
0
作者：
Biddanda, Bopaiah;Weinke, Anthony;Stone, Ian;Ruberg, Steven;Hartmeyer, Phil
通讯作者：
Hartmeyer, Phil

Microbial mats: Extraterrestrial Life Models.

微生物垫：外星生命模型。

DOI：
发表时间：
2021
期刊：
Encyclopedia
影响因子：
0
作者：
Biddanda, B. A.;Weinke, A.;Stone, I;Kendall, S.;Hartmeyer, P.;Lusardi, W.;Gandulla, S.;Bright, J.;and Ruberg, S.
通讯作者：
and Ruberg, S.

A glimpse of the otherworldly “lakescape” at the bottom of the Middle Island Sinkhole in Lake Huron.

一睹休伦湖中岛天坑底部超凡脱俗的“湖景”。