RAPID: A Surface-Based Detection Platform for SARS-CoV-2

RAPID：基于表面的 SARS-CoV-2 检测平台

• 批准号: 2027554
• 负责人: Lia Stanciu
• 金额: $ 9.7万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-15 至 2021-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2027554&HistoricalAwards=false
• 关键词: RAPID; Surface; Based; Detection; Platform

Millions are at risk of severe health issues and even death caused by coronavirus infection (COVID-19 disease). The World Health Organization has declared COVID-19 outbreak a pandemic and an international public health emergency. In response to this situation, Prof. Stanciu at Purdue University aims to design strategies that will result in a point-of-need testing platform for rapid, sensitive, and effective detection of the coronavirus (SARS-CoV-2) in saliva. This project provides opportunity to students to conduct research that integrates electrochemistry, biology, and device design to increase fundamental knowledge for advancing biosensing technologies.The sensor design is based on the measurement of impedance changes upon specific hybridization of surface-immobilized nucleic acid probes with target viral RNA. An important aim of this research is to understand the electrokinetics and fundamental science that strongly influence probe hybridization efficiency. This information is critical for achieving rapid (minutes), highly selective, and highly sensitive detection of the viral RNA (without amplification) under physiological conditions. This biosensing platform can be integrated into field-deployable devices or remote monitoring systems connected to cellular networks that will transmit information to disease control centers.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

数以百万计的风险有严重的健康问题，甚至是冠状病毒感染引起的死亡（Covid-19疾病）。世界卫生组织已宣布Covid-19爆发是大流行和国际公共卫生紧急情况。 为了应对这种情况，普渡大学的Stanciu教授旨在设计策略，从而导致唾液中冠状病毒（SARS-COV-2）的快速，敏感和有效检测的必要测试平台。 该项目为学生提供了将电化学，生物学和设备设计整合的研究的机会，以增加基本知识，以推进生物传感技术。该传感器设计基于测量阻抗变化对表面型核酸核酸探针与目标病毒RNA的特定杂交的测量。 这项研究的一个重要目的是了解强烈影响探测杂交效率的电动和基础科学。 此信息对于在生理条件下对病毒RNA（无扩增）的快速（分钟），高度选择性和高度敏感的检测至关重要。该生物传感平台可以集成到连接到蜂窝网络的远程部署设备或远程监控系统中，这些设备将信息将信息传输到疾病控制中心。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来进行评估的。