Viscoelastic Characterization of Multicellular Tissues in Physiologically Relevant Conditions through Probe Indentation

通过探针压痕对生理相关条件下的多细胞组织进行粘弹性表征

基本信息

批准号：
2019507
负责人：
Santiago Solares
金额：
$ 49.25万
依托单位：
George Washington University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019507&HistoricalAwards=false
关键词：
Viscoelastic Characterization Multicellular Tissues Physiologically

项目摘要

This award will produce new knowledge about the mechanical behavior of cancer tissues. Specifically, microscopic measurements of mechanical behavior will be made as the disease progresses. In this project, the focus will be on skin cancers. However, the findings will be applicable to other diseases for which tissues undergo mechanical changes such as arteriosclerosis. The project will deliver the new methods and sensors capable of use in the native liquid environment of biological materials. Establishing a link between cancer progression and mechanical changes in tissues could ultimately have applications to human health. For example, the results of this work could enable the development of patient-specific disease databases, and standards for mechanical tissue behaviors. These might eventually allow classification by individual patient history and information. This could have a transformative effect on medicine and biology research, as mechanical information is not yet broadly considered, much less classified in engineering terms. This research involves several disciplines, including medicine, biology, materials science and mechanical engineering. The multi-disciplinary approach will help broaden participation of underrepresented groups in research and positively impact engineering education. Biological materials are viscoelastic and may respond according to multiple characteristic timescales which are generally not captured in existing mechanical characterization methods, especially micro- and nanoscale approaches. Such methods often reduce the mechanical response to elastic quantities, such as the Young’s modulus, or combinations of a few quantities that empirically combine dissipative and elastic behaviors. This project will develop viscoelastic property inversion methods, whereby the sample is probed using a microscale indenter, and the measured response is translated into a generalized viscoelastic model containing an arbitrary number of characteristic times. This approach will allow quantification of material behaviors in engineering units, which will allow comparison of different specimens measured on different instruments by different researchers. The project also includes development of the indentation apparatus, which will resemble an atomic force microscope, but will be tailored to the typical relaxation timescales observed in biological materials, which are significantly larger than those probed by commercial atomic force microscopes. The project will involve computational and analytical modeling of the fluid dynamics surrounding the probe and sample to incorporate corrections due to fluid forces, which can in some cases obscure the probe-sample interactions for soft materials. Finally, the new apparatus and methods will be employed to acquire the viscoelastic signature of melanoma cancer and healthy skin cell lines.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项将产生有关癌症组织机械行为的新知识，具体来说，随着疾病的进展，将进行机械行为的微观测量，但研究结果将适用于其他癌症。该项目将提供能够在生物材料的天然液体环境中使用的新方法和传感器，以治疗组织发生机械变化的疾病，最终可以应用于人类健康。例如，结果这项工作可以开发特定于患者的疾病数据库以及机械组织行为标准，这可能最终允许根据个体患者的病史和信息进行分类，这可能会对医学和生物学研究产生变革性的影响，因为机械信息还没有。这项研究涉及多个学科，包括医学、生物学、材料科学和机械工程，这将有助于扩大代表性不足的群体对生物材料研究的参与。粘弹性，可根据多种特性做出响应现有的机械表征方法通常无法捕获时间尺度，特别是微米和纳米尺度的方法，这些方法通常会降低对弹性量的机械响应，例如杨氏模量，或根据经验结合耗散和弹性行为的几个量的组合。该项目将开发粘弹性属性反演方法，使用微型压头探测样品，并将测量到的响应转换为包含任意数量特征时间的广义粘弹性模型。以工程单位量化材料行为，这将允许比较不同研究人员在不同仪器上测量的不同样本，该项目还包括压痕装置的开发，该装置将类似于原子力显微镜，但将根据典型的弛豫时间尺度进行定制。该项目将涉及对探针和样品周围的流体动力学进行计算和分析建模，以纳入流体力引起的校正，这在某些情况下可能会掩盖流体力。探针-样品相互作用最后，新的设备和方法将用于获取黑色素瘤癌症和健康皮肤细胞系的粘弹性特征。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响进行评估，被认为值得支持。审查标准。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Shear-induced migration of a viscous drop in a viscoelastic liquid near a wall at high viscosity ratio: Reverse migration

高粘度比的粘弹性液体中粘弹性液体中粘性液滴的剪切引起的迁移：反向迁移

DOI：
10.1016/j.jnnfm.2022.104751
发表时间：
2022
期刊：
Journal of Non-Newtonian Fluid Mechanics
影响因子：
3.1
作者：
Mukherjee, Swarnajay;Tarafder, Anik;Malipeddi, Abhilash Reddy;Sarkar, Kausik
通讯作者：
Sarkar, Kausik

Shear-induced gradient diffusivity of a red blood cell suspension: effects of cell dynamics from tumbling to tank-treading

红细胞悬浮液的剪切诱导梯度扩散：细胞动力学从翻滚到踩罐的影响