Viscoelastic Characterization of Multicellular Tissues in Physiologically Relevant Conditions through Probe Indentation

通过探针压痕对生理相关条件下的多细胞组织进行粘弹性表征

基本信息

批准号：
2019507
负责人：
Santiago Solares
金额：
$ 49.25万
依托单位：
George Washington University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019507&HistoricalAwards=false
关键词：
Viscoelastic Characterization Multicellular Tissues Physiologically

项目摘要

This award will produce new knowledge about the mechanical behavior of cancer tissues. Specifically, microscopic measurements of mechanical behavior will be made as the disease progresses. In this project, the focus will be on skin cancers. However, the findings will be applicable to other diseases for which tissues undergo mechanical changes such as arteriosclerosis. The project will deliver the new methods and sensors capable of use in the native liquid environment of biological materials. Establishing a link between cancer progression and mechanical changes in tissues could ultimately have applications to human health. For example, the results of this work could enable the development of patient-specific disease databases, and standards for mechanical tissue behaviors. These might eventually allow classification by individual patient history and information. This could have a transformative effect on medicine and biology research, as mechanical information is not yet broadly considered, much less classified in engineering terms. This research involves several disciplines, including medicine, biology, materials science and mechanical engineering. The multi-disciplinary approach will help broaden participation of underrepresented groups in research and positively impact engineering education. Biological materials are viscoelastic and may respond according to multiple characteristic timescales which are generally not captured in existing mechanical characterization methods, especially micro- and nanoscale approaches. Such methods often reduce the mechanical response to elastic quantities, such as the Young’s modulus, or combinations of a few quantities that empirically combine dissipative and elastic behaviors. This project will develop viscoelastic property inversion methods, whereby the sample is probed using a microscale indenter, and the measured response is translated into a generalized viscoelastic model containing an arbitrary number of characteristic times. This approach will allow quantification of material behaviors in engineering units, which will allow comparison of different specimens measured on different instruments by different researchers. The project also includes development of the indentation apparatus, which will resemble an atomic force microscope, but will be tailored to the typical relaxation timescales observed in biological materials, which are significantly larger than those probed by commercial atomic force microscopes. The project will involve computational and analytical modeling of the fluid dynamics surrounding the probe and sample to incorporate corrections due to fluid forces, which can in some cases obscure the probe-sample interactions for soft materials. Finally, the new apparatus and methods will be employed to acquire the viscoelastic signature of melanoma cancer and healthy skin cell lines.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项将产生有关癌组织机械行为的新知识。具体而言，随着疾病的进展，将对机械行为进行微观测量。在这个项目中，重点将放在皮肤癌上。但是，这些发现将适用于其他疾病，这些疾病会发生机械变化，例如动脉硬化。该项目将提供能够在生物材料的天然液体环境中使用的新方法和传感器。建立癌症进展与定时机械变化之间的联系最终可能在人类健康中应用。例如，这项工作的结果可以使患者特异性疾病数据库的发展以及机械组织行为的标准。这些最终可能允许通过个别患者的病史和信息进行分类。这可能会对医学和生物学研究产生变革性的影响，因为尚未广泛考虑机械信息，因此在工程术语方面进行了较少的分类。这项研究涉及几个学科，包括医学，生物学，材料科学和机械工程。多学科的方法将有助于扩大代表性不足的群体在研究中的参与，并积极影响工程教育。生物材料是粘弹性的，可能会根据多个特征时间尺度做出响应，这些时间尺度通常未在现有的机械表征方法中捕获，尤其是微观和纳米级方法。这种方法通常会减少对弹性量的机械响应，例如杨氏模量，或者急切地结合耗散性和弹性行为的少量组合。该项目将开发粘弹性属性反转方法，从而使用微观缩进器探测样品，并将测量响应转换为包含任意数量特征时间时的广义粘弹性模型。这种方法将允许量化工程单位中的材料行为，这将允许比较不同研究人员在不同工具上测量的不同物种。该项目还包括开发凹痕设备，该设备将类似于原子力显微镜，但将根据在生物学材料中观察到的典型弛豫时间尺度量身定制，该材料的典型弛豫时间表明显大于商业原子力显微镜所探测的时间。该项目将涉及围绕探针和样品的流体动力学的计算和分析建模，以结合流体力引起的校正，在某些情况下，这可能会掩盖软材料的探针样本相互作用。最后，将采用新的设备和方法来获取黑色素瘤癌症和健康皮肤细胞系的粘弹性特征。该奖项反映了NSF的法定任务，并使用基金会的智力优点和更广泛的影响审查标准，被视为通过评估来获得珍贵的支持。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Shear-induced migration of a viscous drop in a viscoelastic liquid near a wall at high viscosity ratio: Reverse migration

高粘度比的粘弹性液体中粘弹性液体中粘性液滴的剪切引起的迁移：反向迁移

DOI：
10.1016/j.jnnfm.2022.104751
发表时间：
2022
期刊：
Journal of Non-Newtonian Fluid Mechanics
影响因子：
3.1
作者：
Mukherjee, Swarnajay;Tarafder, Anik;Malipeddi, Abhilash Reddy;Sarkar, Kausik
通讯作者：
Sarkar, Kausik

Shear-induced gradient diffusivity of a red blood cell suspension: effects of cell dynamics from tumbling to tank-treading

红细胞悬浮液的剪切诱导梯度扩散：细胞动力学从翻滚到踩罐的影响