Shape adaptable, non-centrosymmetric viral scaffolds for assembly of self-propelled nanomaterials

用于组装自驱动纳米材料的形状适应性强、非中心对称的病毒支架

基本信息

批准号：
2002941
负责人：
Elaine Haberer
金额：
$ 34.2万
依托单位：
University of California-Riverside
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-15 至 2024-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2002941&HistoricalAwards=false
关键词：
Shape adaptable non centrosymmetric viral

项目摘要

Non-technical Summary: Synthetic swimmers have been increasingly explored. These remarkable self-propelled materials can convert chemical or light energy into locomotion, increasing mixing and accelerating chemical reactions. Asymmetry in particle composition enables movement through a local build-up of reaction products, whereas particle size and geometry control speed and directionality. While motors with dimensions of 100s of nanometers or microns have been widely studied, sub-100 nm devices have been largely neglected because they are harder to make. This research will use the expert manufacturing capabilities of viral biomaterials to overcome these fabrication challenges. The development of such an assembly strategy will enable the spatial and topographical control essential for important biomedical, sensing, and environmental remediation applications. This research will support both mentoring and outreach activities to inspire, recruit, and train a diverse group of scientists and engineers. These activities will strengthen the education of graduate, undergraduate, and middle students.Technical Summary: Viral nanoparticles are monodisperse, self-assembled biomaterials. Their structure and chemistry, including exact shape and site-specific functional groups, is genetically encoded and known with precision. The project objective is to create a shape-changing viral template with broken symmetry that is capable of serving as a platform with which to design and synthesize asymmetric functional nanomaterials. The proposed work will focus on using the size and shape of the transformed viral templates to manipulate the motion of viral nanoparticle-based nanoswimmers. Viral geometry will be converted from filament to rod to spheroid through brief organic solvent exposure. Following these extreme dimensional changes and sidechain packing transitions, differences in surface exposed residues will be evaluated. In addition, the effect of N-terminal amino acid sidechain character on solvent-based transformation, as well as the size and stability of the shape-modified scaffold will be studied. The reduced surface chemistry homogeneity of the shape-changed viruses will be used to synthesize or assemble non-centrosymmetric self-electrophoretic and light-induced self-electrophoretic nanoparticle-based nanoswimmers. Viral-templated nanoparticle trajectory will be explored, rotational and translational diffusion coefficients measured, and motion correlated with nanomaterial size and shape. The proposed studies will improve fundamental understanding of the structure-function relationship associated with geometric tunability of the filamentous virus and particle motion; advance knowledge of biomolecule interactions with and control over non-centrosymmetric nanostructure formation; and enhance viral-templated nanoparticle motion through precise control over template size and shape.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

非技术摘要：越来越多地探索了合成游泳者。这些显着的自露的材料可以将化学或光能转化为运动，从而增加混合和加速化学反应。粒子组成中的不对称性可以通过反应产物的局部积聚，而粒径和几何控制速度和方向性。尽管已经广泛研究了具有100纳米或微米的尺寸的电动机，但由于难以制造，因此已被忽略了100 nm的设备。这项研究将使用病毒生物材料的专家制造能力来克服这些制造挑战。这种装配策略的发展将使重要的生物医学，感应和环境补救应用必不可少的空间和地形控制。这项研究将支持指导和推广活动，以激发，招募和培训一群科学家和工程师。这些活动将加强研究生，本科生和中级学生的教育。技术摘要：病毒纳米颗粒是单分散的，自组装的生物材料。它们的结构和化学（包括精确的形状和特定位置官能团）是遗传编码并精确的。该项目的目标是创建一个带有破碎的对称性的变形病毒模板，该模板能够用作设计和合成不对称功能纳米材料的平台。拟议的工作将集中于使用转化的病毒模板的大小和形状来操纵基于病毒的纳米粒子的运动。病毒几何形状将通过短暂的有机溶剂暴露从细丝到杆转换为球体。遵循这些极端维度的变化和侧chain填料过渡，将评估表面暴露残基的差异。此外，还将研究N末端氨基酸Sidechain特征对基于溶剂的转化以及形状改性支架的大小和稳定性的影响。形状变化病毒的表面化学同质性降低将用于合成或组装非中心对称的自我电泳和光诱导的自发遗传纳米粒子基于纳米晶体。将探索病毒式纳米颗粒轨迹，旋转和转移扩散系数，运动与纳米材料的大小和形状相关。拟议的研究将提高对与丝状病毒和颗粒运动的几何可调性相关的结构功能关系的基本理解。对非中心纳米结构形成的生物分子相互作用的提高知识；并通过精确控制模板大小和形状来增强病毒式的纳米颗粒运动。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响来通过评估来支持的。