Collaborative Research: The brood cell microbiome of solitary bees: origin, diversity, function, and vulnerability

合作研究：独居蜜蜂的巢细胞微生物组：起源、多样性、功能和脆弱性

基本信息

批准号：
1929499
负责人：
Bryan Danforth
金额：
$ 61.3万
依托单位：
Cornell University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1929499&HistoricalAwards=false
关键词：
Collaborative Research brood cell microbiome

项目摘要

Bees are the single most important pollinators of flowering plants worldwide. Over 85% of the 325,000 flowering plant species on earth depend on animals for pollination, and the vast majority of pollination is carried out by bees. Annually, bees are estimated to contribute $15 billion to US crop production and $170 billion to global crop production. High-value bee-pollinated crops include apple and other early spring tree fruits, strawberries, blueberries, cherries, cranberries, squash and pumpkins, tomatoes, almonds, and many others. The economic viability of US agricultural production is dependent on stable and healthy wild and domesticated bee populations. However, bee populations are threatened by a variety of factors, including habitat loss, pathogen spillover, invasive plants and animals, and pesticide use, which can disrupt the normal microbial symbionts essential for bee larval development (the "brood cell" microbiome). This research project focuses on understanding what role microbes play in the larval nutrition in a wide variety of bee species. Previous research has documented a diverse community of bacteria and yeasts in the pollen and nectar diet of bees. As larvae consume these pollen/nectar provisions they are ingesting microbes, and our preliminary results indicate that these microbes form an essential component of the larval diet. This project has the potential to significantly modify how we view the 120 million-year-old partnership between bees and flowering plants, and will provide essential information for developing long-term bee conservation efforts. Project outreach efforts include educational activities on solitary bees for K-12 students and interactive demonstrations of bee-microbe-flower interactions for broad audiences. The project will use cutting-edge methods to (1) document the microbial diversity in flowers and pollen provisions, (2) determine the nutritional role of microbes in larval development and health, and (3) understand how alterations in microbial community impact larval development. To document microbial diversity in both host-plant flowers and pollen provisions, the research team will use amplicon sequencing and microbial metagenomics. These methods will document the microbial species present in pollen provisions as well as the metabolic activities these microbes perform during pollen maturation. Screening the pollen and nectar of host-plant species will provide key insights into the source of the brood cell microbiome. To determine the nutritional role of the microbial community the research team will use two methods from trophic ecology: compound specific isotope analysis and neutral lipid fatty acid analysis. These analyses will permit the research team to track the origin (floral or microbial) of amino acids and fatty acids in the larval diet of 15 focal bee species. Finally, through manipulative laboratory experiments the research team will determine how modifications of the microbial communities impact larval development. Combining the results of these studies will provide a comprehensive understanding of how bees and flowering plants interact via their shared microbial partners. This project is jointly funded by the Systematics and Biodiversity Sciences Cluster (Division of Environmental Biology) and the Symbiosis, Defense and Self-recognition Program (Division of Integrative Organismal Systems).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

蜜蜂是全世界开花植物最重要的传粉媒介。地球上325,000种开花植物中超过85%依靠动物授粉，而绝大多数授粉是由蜜蜂进行的。据估计，蜜蜂每年为美国农作物生产贡献 150 亿美元，为全球农作物生产贡献 1700 亿美元。高价值的蜜蜂授粉作物包括苹果和其他早春树果、草莓、蓝莓、樱桃、蔓越莓、南瓜和南瓜、西红柿、杏仁等。美国农业生产的经济可行性取决于稳定、健康的野生和驯养蜜蜂种群。然而，蜜蜂种群受到多种因素的威胁，包括栖息地丧失、病原体溢出、入侵植物和动物以及农药的使用，这些因素可能会破坏蜜蜂幼虫发育所必需的正常微生物共生体（“巢细胞”微生物组）。该研究项目的重点是了解微生物在多种蜜蜂幼虫营养中发挥的作用。先前的研究已经记录了蜜蜂的花粉和花蜜饮食中存在多种细菌和酵母菌群。当幼虫消耗这些花粉/花蜜时，它们正在摄入微生物，我们的初步结果表明这些微生物构成了幼虫饮食的重要组成部分。该项目有可能显着改变我们对蜜蜂和开花植物之间长达 1.2 亿年的伙伴关系的看法，并将为开展长期的蜜蜂保护工作提供重要信息。项目推广工作包括为 K-12 学生举办有关独居蜜蜂的教育活动，以及为广大观众展示蜜蜂-微生物-花相互作用的互动演示。该项目将使用尖端方法（1）记录花朵和花粉中的微生物多样性，（2）确定微生物在幼虫发育和健康中的营养作用，以及（3）了解微生物群落的变化如何影响幼虫发育。为了记录寄主植物花朵和花粉的微生物多样性，研究小组将使用扩增子测序和微生物宏基因组学。这些方法将记录花粉中存在的微生物种类以及这些微生物在花粉成熟过程中进行的代谢活动。筛选寄主植物物种的花粉和花蜜将为了解巢细胞微生物组的来源提供重要见解。为了确定微生物群落的营养作用，研究小组将使用营养生态学的两种方法：化合物特异性同位素分析和中性脂质脂肪酸分析。这些分析将使研究小组能够追踪 15 种重点蜜蜂幼虫饮食中氨基酸和脂肪酸的来源（花卉或微生物）。最后，通过实验室实验，研究小组将确定微生物群落的改变如何影响幼虫的发育。结合这些研究的结果将提供对蜜蜂和开花植物如何通过它们共享的微生物伙伴相互作用的全面了解。该项目由系统学和生物多样性科学集群（环境生物学部门）和共生、防御和自我识别计划（综合有机系统部门）共同资助。该奖项反映了 NSF 的法定使命，并被认为值得通过以下方式支持：使用基金会的智力价值和更广泛的影响审查标准进行评估。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nectar compounds impact bacterial and fungal growth and shift community dynamics in a nectar analog

花蜜化合物影响细菌和真菌的生长，并改变花蜜类似物中的群落动态

DOI：
10.1111/1758-2229.13139
发表时间：
2023-02
期刊：
Environmental Microbiology Reports
影响因子：
3.3
作者：
Mueller, Tobias G.;Francis, Jacob S.;Vannette, Rachel L.
通讯作者：
Vannette, Rachel L.

Comparative genomics reveals that metabolism underlies evolution of entomopathogenicity in bee-loving Ascosphaera spp. fungi

比较基因组学揭示，代谢是喜爱蜜蜂的子囊菌属昆虫致病性进化的基础。

DOI：
10.1016/j.jip.2022.107804
发表时间：
2022-10
期刊：
Journal of Invertebrate Pathology
影响因子：
3.4
作者：
Maccaro, J.J.;Moreira Salgado, J.F.;Klinger, E.;Argueta Guzmán, M.P.;Ngor, L.;Stajich, J.E.;McFrederick, Q.S.
通讯作者：
McFrederick, Q.S.

Microbes make the meal: oligolectic bees require microbes within their host pollen to thrive

微生物制造膳食：寡聚蜜蜂需要宿主花粉中的微生物才能茁壮成长

DOI：
10.1111/een.12926
发表时间：
2020-12
期刊：
Ecological Entomology
影响因子：
2.2
作者：
Dharampal, Prarthana S.;Hetherington, Matthew C.;Steffan, Shawn A.
通讯作者：
Steffan, Shawn A.

Incipiently social carpenter bees ( Xylocopa ) host distinctive gut bacterial communities and display geographical structure as revealed by full‐length PacBio 16S rRNA sequencing

全长 PacBio 16S rRNA 测序揭示了早期社会性木蜂 (Xylocopa) 拥有独特的肠道细菌群落，并显示出地理结构

DOI：
10.1111/mec.16736
发表时间：
2023-03
期刊：
Molecular Ecology
影响因子：
4.9
作者：
Handy, Madeline Y.;Sbardellati, Dino L.;Yu, Michael;Saleh, Nicholas W.;Ostwald, Madeleine M.;Vannette, Rachel L.
通讯作者：
Vannette, Rachel L.

The Floral Microbiome: Plant, Pollinator, and Microbial Perspectives

花卉微生物组：植物、传粉媒介和微生物的观点