CAREER: Mechanisms and costs of temperature adaptation along a latitudinal cline for the coastal copepod, Acartia tonsa

职业生涯：沿海桡足类 Acaria tonsa 沿纬度梯度的温度适应机制和成本

基本信息

批准号：
1943316
负责人：
Melissa Pespeni
金额：
$ 145.58万
依托单位：
University of Vermont & State Agricultural College
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1943316&HistoricalAwards=false
关键词：
CAREER Mechanisms costs temperature adaptation

项目摘要

Organisms that live in environments that vary across space or time may have greater capacity to acclimate or adapt to rapid changes in environmental conditions. However, little is known about the mechanisms or capacities for rapid adaptation among populations or the extent of costs associated with rapid evolution. This project will characterize the mechanisms and costs of natural and experimental temperature adaptation using multiple populations along the broad latitudinal distribution of the foundational coastal copepod, Acartia tonsa. This work will also greatly advance our general understanding of variation in capacity to adapt for many other ecologically and economically critical species. To carry out this work, the principal investigator will lead 100 undergraduates, over the five-year period, through an integrative teaching-research asset-based training program that connects a colloquium, summer research internship, and research course. In addition, two PhD students, one postdoctoral scientist, and one research technician will develop diverse skillsets in asset-based mentoring, genetics, genomics, evolution, computational and marine biology, and how to be a member of an inclusive, supportive STEM community. In addition to improving the well-being of participants and growing a more diverse STEM workforce, results from the research will benefit society by improving the ability to predict species persistence in future conditions and improving understanding of the scope of the threat of changing global conditions on ocean ecosystems.Populations respond to environmental conditions that vary across space and time through genetic adaptation or physiologically plasticity, however, a comprehensive understanding of how these mechanisms vary across populations or contribute to future resilience is lacking. In addition, temperature adaptation is a complex physiological trait, thus rapid temperature adaptation could take multiple paths depending on the genomic background of a population and could yield unintended costs. In the project, the principal investigator will lead students in work to test the hypotheses that variation in genomic backgrounds affects capacity to adapt and that rapid adaptation to one stressor comes with costs to survive other stressors. The project will capitalize on the broad latitudinal distribution and experimental tractability of the ecologically foundational coastal copepod, Acartia tonsa. The researchers will 1) Link genotype to temperature adaptive phenotype using ecological genomics and genome-wide association studies in populations along the environmental gradient of the U.S. East Coast, 2) Reveal the transcriptional bases of differences in thermal tolerance among populations and life-history stages, 3) Characterize variation in temperature adaptation trajectories in multiple experimentally evolved populations and measure the costs of adaptation. Integration of results across these aims will lead to insights to the molecular bases of complex traits, the repeatability of evolutionary trajectories in lab- and field-based studies, and the relationship between plastic and genetic response mechanisms in temperature adaptation. Lastly, this work will be performed through the development of an integrated teaching-research training program that will increase diversity and inclusion in a STEM research community. This grant was cofunded by the Integrative Ecological Physiology Program in the Division of Integrative Organismal Systems in the Directorate for Biological Science, The Biological Oceanography Program in the Division of Ocean Sciences in the Directorate for Geosciences, and The EPSCoR Program in the Office of Integrative Activities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生活在各个时空或时间各不相同的环境中的生物可能具有更大的能力来适应或适应环境条件的快速变化。但是，关于人群快速适应或与快速进化相关的成本程度的机制或能力知之甚少。该项目将使用沿着基础沿海copepod的宽纬度分布Acartia Tonsa的宽纬度分布进行自然和实验温度适应的机制和成本。这项工作还将大大提高我们对适应许多其他生态和经济上关键物种的能力差异的一般理解。为了进行这项工作，首席研究员将在五年内通过基于综合教学研究资产的培训计划领导100名本科生，该计划将座谈会，夏季研究实习和研究课程连接起来。此外，两名博士学位学生，一名博士后科学家以及一位研究技术人员将在基于资产的指导，遗传学，基因组学，进化，计算和海洋生物学方面发展各种技能，以及如何成为包容性，支持性STEM社区的成员。除了改善参与者的福祉和增加更多样化的STEM劳动力外，该研究的结果还将通过提高预测物种在未来条件下的物种持久性的能力，并改善对整个海洋生态系统变化的威胁范围的理解的理解，而对海洋生态系统的变化范围。然而，对环境的构成范围和时间跨越了跨越的环境，这些范围跨越了跨越的环境，而构成了这些范围，那么这些范围跨越了量化，这些塑料的范围是遗传适应性的，这些机构是对这些范围的范围，这些范围众所周知，这些塑料的范围各不相同缺乏弹性。此外，温度适应是一个复杂的生理特征，因此，快速温度适应可能会根据人群的基因组背景采取多个途径，并且可能产生意外的成本。在该项目中，主要研究人员将领导学生进行工作，以测试基因组背景变化会影响适应能力的假设，并且快速适应一种压力源，伴随着生存其他压力源的成本。该项目将利用生态基础的沿海Copepod Acartia Tonsa的广泛纬度分布和实验性障碍。研究人员将1）使用生态基因组学和全基因组范围的结合研究将基因型与温度自适应表型联系起来，沿美国东海岸的环境梯度的人群中的研究，2）揭示了人群和使用寿命阶段的热耐受性差异的转录基础，3）在多种经验中的温度适应轨迹中的体质变化构成了多种经验型群体的变化。这些目标之间的结果整合将导致对复杂性状的分子碱基的见解，基于实验室和野外研究中进化轨迹的可重复性以及塑性与温度适应中塑性反应机制之间的关系。最后，这项工作将通过制定集成的教学培训计划来进行，该计划将增加在STEM研究社区中的多样性和包容性。这项赠款是由整合性生态生理学计划在整合有机系统部门的生物科学局，地球科学局海洋科学局的生物海洋学计划以及EPSCOR计划中的综合活动办公室计划中造成的。NSF的法定任务和审查均通过评估范围，这表明了Intelpriatiac and Infectial and Infectia the Infectiac and Infectial the Infectiac and Infectiatial reveriation te Infectia and Infectiatial te Infectiatiac and Infectiation均表现出色。