Global key players of chloroplast gene expression

叶绿体基因表达的全球关键参与者

• 批准号: 238561773
• 负责人: Privatdozent Dr. Jörg Meurer
• 金额: --
• 依托单位: Lehrstuhl für Molekularbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/238561773?language=en
• 关键词: Global; key; players; chloroplast; gene

The chloroplast Ribonuclease E (RNE) plays an important key role in processing of many transcripts and therefore belongs to the major RNA processing factors in chloroplasts. By means of forward and reverse genetics in Arabidopsis and tagging approaches in combination with mass spectrometry we recently identified a degradosome-like complex (DLC), which contains RNE, RNJ, one helicase, two ribosomal proteins with presumably extraribosomal functions and unknown proteins, like RHON1 and a pentatricopeptide repeat (PPR) protein. A degradosome was previously thought not to exist in chloroplasts. The essential and plant specific RHON1 protein was found to bind specifically to those RNA regions, which are not processed efficiently in rne and rhon1 mutants. Therefore, we hypothesize that RHON1 supports the function of RNE presumably by delivering the target RNAs to the DLC. Interestingly, rne and rhon1 mutants have numerous and tiny chloroplasts strongly supporting an important role in plastid division. Another global player in plastid gene expression is represented by pale cress (PAC). We showed that PAC directly interacts with proteins and RNA. This project aims to analyse the function and importance of interaction partners of RNE, RHON1 and PAC, to identify the precise RNA targets of PAC and to investigate a putative regulatory role in plastid gene expression and the function of PAC in ribosome assembly. We anticipate that future research on the DLC and PAC, their compartmentalization within the chloroplast and association partners will shed new light on chloroplast gene expression, division and development.

叶绿体核糖核酸酶E（RNE）在处理许多转录物中起重要作用，因此属于叶绿体中的主要RNA加工因子。通过拟南芥中的正向和反向遗传学和标记方法与质谱法结合使用，我们最近确定了一种降解体样复合物（DLC），其中包含RNE，RNJ，一种旋转酶，两种核糖体蛋白，具有大概是外颌外功能的核糖体蛋白质，并且可能是rhon1和rhon1和rhon1和ppr reprete ppr）。以前认为降解体在叶绿体中不存在。发现必需的和植物特异性的Rhon1蛋白特异性结合了那些在RNE和RHON1突变体中没有有效处理的RNA区域。因此，我们假设RHON1通过将目标RNA传递到DLC来支持RNE的功能。有趣的是，RNE和RHON1突变体具有大量且微小的叶绿体，强烈支持在质体分裂中发挥重要作用。 Pale Cress（PAC）代表了另一个全球质体基因表达的参与者。我们表明PAC与蛋白质和RNA直接相互作用。该项目旨在分析RNE，RHON1和PAC的相互作用伙伴的功能和重要性，以确定PAC的精确RNA靶标，并研究PAC在质体基因表达中的推定调节作用以及PAC在核糖体组装中的功能。我们预计，未来对DLC和PAC的研究，它们在叶绿体和协会伴侣中的分区化将为叶绿体基因的表达，分裂和发展提供新的启示。