Development of in vivo NMR techniques for studying the impact of Ocean acidification and warming on the neurophysiology of Antartic fishes

开发体内核磁共振技术来研究海洋酸化和变暖对南极鱼类神经生理学的影响

基本信息

批准号：
237813768
负责人：
Dr. Christian Bock
金额：
--
依托单位：
Alfred-Wegener-Institut (AWI) Helmholtz-Zentrum für Polar- und Meeresforschung
依托单位国家：
德国
项目类别：
Infrastructure Priority Programmes
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/237813768?language=en
关键词：
Development vivo NMR techniques studying

项目摘要

The most dramatic effects of ocean acidification and warming are expected in Polar regions, i.e., the impact of elevated temperatures and CO2 concentrations will be highest on Polar organisms (Antarctic and Arctic species). It was demonstrated that tropical fishes show neurological impairments under elevated CO2 concentrations expected from climate change scenarios for 2100 already, although fishes have a decent acid-base regulation. The impairments involved different neurological dysfunctions from sensory to behavioural changes and were supposed to be due to the influence of CO2 on the neurotransmitter function of GABA. Global warming might amplify these disturbances. Thus, in Antarctic eelpout it was shown that acid-base regulation of intracellular pH became impaired at temperatures above 4°C.Polar fishes can show similar or more severe neurological changes under conditions of ocean acidification and warming. However, the mechanisms responsible for the observed disturbances of marine fishes are still not understood. In vivo NMR techniques, in particular localised 31P and 1H NMR spectroscopy were extensively used to characterise the metabolic status or metabolic changes in the brain of animals and humans, including observations on changes of neurotransmitters as well as acid-base regulation. However, these measurements are limited by the inherently low sensitivity, restricting the spatial and temporal resolution.In the current research project, the prolongation of which we are applying for, we exploit the novel approach of chemical shift saturation transfer (CEST) for pH mapping and indirect metabolic imaging. CEST magnetic resonance imaging (MRI) allows measurements with high spatial and temporal resolution because the saturated longitudinal magnetisation of exchangeable protons of metabolites is transferred to water and accumulated. We have shown that CEST MRI is feasible down to polar temperatures and that the specificity of CEST MRI can be adjusted by appropriate radiofrequency (RF) pre-saturation, allowing both inverse imaging of important metabolites and pH mapping. Besides CEST MRI based on glutamate (GluCEST), we plan to develop CEST MRI based on taurine (TauCEST). TauCEST will be a novel CEST option, which seems to be applicable at low temperatures. Using the new 9.4T NMR imaging system, which is expected to be operational in January, 2016, at the AWI, will allow in vivo measurements with improved quality. Both CEST MRI and localized 1H and 31P MRS will be used for studying metabolic changes and acid-base regulation in the brain of Antarctic fishes under expected ocean acidification and warming scenarios. These studies should improve our understanding of the mechanisms underlying the observed neurological changes in Polar fishes.

海洋酸化和变暖的影响预计在极地地区最为显着，即气温升高和二氧化碳浓度升高对极地生物（南极和北极物种）的影响最大。事实证明，热带鱼在二氧化碳浓度升高的情况下会表现出神经损伤。尽管鱼类具有良好的酸碱调节能力，但其浓度已经达到了 2100 年气候变化情景中预期的水平，这些损害涉及从感觉到行为变化的不同神经功能障碍，并且被认为是由于二氧化碳对鱼类的影响。 GABA 的神经递质功能可能会加剧这些干扰。因此，在南极洲 elpout 中，细胞内 pH 值的酸碱调节在温度高于 4°C 时会受到损害。极地鱼类在以下条件下会表现出类似或更严重的神经变化。然而，造成海洋鱼类扰动的机制仍不清楚，特别是常用的局部 31P 和 1H NMR 光谱。表征动物和人类大脑中的代谢状态或代谢变化，包括对神经递质变化以及酸碱调节的观察。然而，这些测量受到固有的低灵敏度的限制，限制了空间和时间分辨率。在当前的研究项目（我们正在申请的扩展）中，我们利用化学位移饱和转移（CEST）的新方法进行 pH 绘图和间接代谢成像（MRI），可以进行高空间和时间分辨率的测量。因为饱和纵向磁化强度我们已经证明，CEST MRI 可以在极地温度下进行，并且可以通过适当的射频 (RF) 预饱和来调整 CEST MRI 的特异性，从而可以对重要代谢物进行反向成像。除了基于谷氨酸的 CEST MRI (GluCEST) 之外，我们还计划开发基于牛磺酸 (TauCEST) 的 CEST MRI。 CEST 选项似乎适用于低温情况，预计将于 2016 年 1 月在 AWI 运行，使用新的 9.4T NMR 成像系统将可以提高 CEST MRI 和局部测量的质量。 1H 和 31P MRS 将用于研究在预期的海洋酸化和变暖情景下南极鱼类大脑的代谢变化和酸碱调节，这些研究应该提高我们对观察到的神经变化背后机制的理解。在极地鱼类中。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Temperature dependence of 1H NMR chemical shifts and its influence on estimated metabolite concentrations

1H NMR 化学位移的温度依赖性及其对估计代谢物浓度的影响

DOI：
10.1007/s10334-017-0642-z
发表时间：
2017
期刊：
Magnetic Resonance Materials in Physics, Biology and Medicine
影响因子：
0
作者：
Wermter FC; Mitschke N; Bock C; Dreher W
通讯作者：
Dreher W

MRI and MRS on preserved samples as a tool in fish ecology.

保存样本的 MRI 和 MRS 作为鱼类生态学的工具

DOI：
10.1016/j.mri.2016.12.017
发表时间：
2017
期刊：
Magnetic resonance imaging
影响因子：
2.5
作者：
Bock C; Wermter FC; Mintenbeck K
通讯作者：
Mintenbeck K

In vivo31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance.

海洋无脊椎动物肌肉生物能学的体内 31P-MRS：未来海洋限制扇贝的性能

DOI：
10.1016/j.mri.2019.06.003
发表时间：
2019
期刊：
Magnetic resonance imaging
影响因子：
2.5
作者：
Bock C; Wermter FC; Schalkhausser B; Blicher ME; Pörtner HO; Lannig G; Sejr MK
通讯作者：
Sejr MK

Investigating GluCEST and its specificity for pH mapping at low temperatures

研究 GluCEST 及其在低温下进行 pH 绘图的特异性