Controlled Synthesis of Polypeptides from Non-Purified N-Carboxyanhydrides

由未纯化的 N-羧酸酐控制合成多肽

• 批准号: 1905097
• 负责人: Paul Braun
• 金额: $ 23.6万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1905097&HistoricalAwards=false
• 关键词: Controlled; Synthesis; Polypeptides; Non; Purified

In this project, funded by the Macromolecular, Supramolecular and Nanochemistry Program in the Division of Chemistry, Professor Jianjun Cheng of the Department of Chemistry at the University of Illinois at Urbana Champaign is developing fast, inexpensive and simple routes for the preparation of synthetic polypeptides using amino acids as the starting materials. Polypeptides are important biopolymers; They consist of long chains of amino acids linked together by a particular kind of a chemical bond called a peptide bond or link. All living organisms are composed of polypeptides. This research allows for the large-scale preparation of polypeptide materials in several hours instead of days. Even more importantly, this new synthetic protocol eliminates the need for purification of starting materials which is often necessary in order to eliminate numerous side reactions that limit the chain length of the desired polypeptides. Successful advancement of this new method has the potential to impact the synthetic peptide synthesis, as well as several other scientific disciplines. Research activities provide undergraduate and graduate students with valuable educational experience in synthetic polymer chemistry. The knowledge of polymerization chemistry and applications will be disseminated to underrepresented undergraduates and K-12 students through the Discover Engineering Summer Camp programs and Engineering Open-House Program at University of Illinois. This research is focused on developing the open-air method for controlled synthesis of polypeptides from non-purified N-carboxyanhydride. Special emphasis is placed on water-free conditions and the elimination of purification steps involving monomers, initiators and catalysts. These steps are often critical in polymer synthesis in order to minimize chain transfer or termination reactions which severely decrease the degree of polymerization of the polypeptides. Each reaction component is carefully analyzed through systematic studies. These polymerizations are applicable to a wide range of amino acids and enable the synthesis of polypeptides with high molecular weights. The in situ purification can potentially be applied to other polymerization techniques to simplify the procedures and lower the materials synthesis costs. This innovative research has a potential to advance industrially-important rapid syntheses of polypeptides.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在这个项目中，由化学系的大分子，超分子和纳米化学计划资助，伊利诺伊大学欧洲冠军大学化学系教授吉安琼·郑教授正在开发快速，廉价且简单的途径，以制备使用氨基酸作为起始材料的合成多肽制备制备合成多肽。 多肽是重要的生物聚合物。它们由长长的氨基酸链组成，该氨基酸由一种称为肽键或环节的特定化学键相连的氨基酸。 所有生物体均由多肽组成。 这项研究允许在几个小时而不是几天内大规模制备多肽材料。 更重要的是，这种新的合成协议消除了纯化起始材料的需求，这通常是必要的，以消除限制所需多肽的链长的大量副反应。 这种新方法的成功发展有可能影响合成肽的合成以及其他几个科学学科。 研究活动为本科生和研究生提供了合成聚合物化学方面的宝贵教育经验。 通过Discover Engineering Summer Camp计划和伊利诺伊大学大学的工程开放式计划，将对聚合化学和应用的知识传播到代表性不足的本科生和K-12学生。这项研究的重点是开发从非纯化的N-羧基氢化物中控制多肽合成的露天方法。 特别强调无水条件以及消除涉及单体，发起人和催化剂的纯化步骤。 这些步骤通常在聚合物合成中至关重要，以最大程度地减少链转移或终止反应，从而严重降低了多肽的聚合程度。 通过系统研究仔细分析每个反应成分。 这些聚合适用于多种氨基酸，并能够合成具有高分子量的多肽。 原位纯化可以可能应用于其他聚合技术，以简化程序并降低材料合成成本。 这项创新的研究有潜力推进多种肽的工业至关重要的快速合成。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响评估标准通过评估来支持的。