ERA-CAPS: Collaborative Research: Thylakoid ion flux-Linking photosynthetic efficiency with osmotic stress response

ERA-CAPS：合作研究：类囊体离子通量-将光合效率与渗透胁迫响应联系起来

• 批准号: 1847193
• 负责人: David Kramer
• 金额: $ 36.08万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1847193&HistoricalAwards=false
• 关键词: ERA; CAPS; Collaborative; Research; Thylakoid

Plant productivity is dependent on many environmental factors, whether plants grow wild or are carefully cultivated in a farmer's field. Efficient photosynthesis is the key to plant productivity, but soil salinity disrupts electrolytes, the so-called ion balance, in the leaf cells where photosynthesis occurs. Soil salinity is a growing problem globally and will affect worldwide food supplies if not effectively controlled. Deciphering how a plant responds to salinity and regulates its cellular ion fluxes is essential to understanding and potentially improving photosynthesis. This project identifies specific genes that control ion fluxes and responses to salinity in plants using a specialized imaging system and analysis tools to study their role in plant productivity. The international team will use their findings to develop a computational model to inform and improve breeding programs and to develop more highly productive plants that can tolerate soil salinity. The model will be tested in tomato with potential to have broad impact to assure global food security.Ion flux across the thylakoid membrane represents a promising target for improving plant photosynthesis, yet knowledge of key components is far from complete. This project brings together an international consortium with complementary expertise in chloroplast ion flux, spectroscopy, phenotyping, ionomics, biochemistry, bioenergetics, and computational modelling. By pursing multi-day phenotyping with dynamic growth lighting, the team has isolated new candidate thylakoid ion flux mutants. The researchers will study the compromised genes and their link to known thylakoid ion flux mediators by pursuing the following aims: (i) complete the thylakoid ion transport protein inventory; (ii) determine the role of thylakoid ion flux interactions in photosynthesis and hyperosmotic stress resistance; (iii) identify the process by which thylakoid ion flux impacts the hyperosmotic stress response; (iv) analyze thylakoid ion flux impacts on key agronomical traits in crop plants; and (v) generate a model for simulating increases to photosynthetic efficiency and salt stress resistance as a function of thylakoid ion flux. The Flux4LIVES project has several broader impacts. Photosynthesis is arguably the most important reaction on earth and a foundation for life. With clear evidence that growth conditions in the field have become more adverse recently, a detailed understanding on how abiotic stress impacts this reaction pathway is needed. Understanding the molecular mechanisms that protect photosynthesis and plant productivity will be key to improving these pathways and thus securing necessary levels of global food production. Since the data obtainedwill be open-access via the existing PhotosynQ database, the global scientific community can apply the knowledge to their own biological and ecological questions and thus further the effort to meet food demands across varying climates and conditions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物生产力取决于许多环境因素，无论是野生植物还是在农民领域仔细种植。有效的光合作用是植物生产力的关键，但是在光合作用的叶细胞中，土壤盐度破坏了所谓的离子平衡。土壤盐度在全球范围内是一个日益增长的问题，如果不有效控制，将影响全球粮食供应。破译植物对盐度的反应并调节其细胞离子通量对于理解和可能改善光合作用至关重要。该项目确定了使用专门的成像系统和分析工具来研究其在植物生产力中的作用的特定基因，这些基因可以控制离子通量和对植物中盐度的反应。国际团队将利用他们的发现来开发一种计算模型，以告知和改善育种计划，并开发能够忍受土壤盐分的高产植物。该模型将在番茄中进行测试，并有可能产生广泛的影响以确保全球粮食安全。在类囊体膜中的ION通量是改善植物光合作用的有希望的目标，但是对关键成分的知识远非完整。该项目汇集了一个国际财团，具有叶绿体通量，光谱，表型，离子学，生物化学，生物能学和计算建模方面的互补专业知识。通过通过动态生长照明进行多天的表型，该团队隔离了新的候选类囊体离子通量突变体。研究人员将通过追求以下目的来研究受损的基因及其与已知的类囊体离子通量介质的联系：（i）完成类囊体离子转运蛋白库存； （ii）确定类囊体离子通量相互作用在光合作用和过度渗透应激性中的作用； （iii）确定类囊体离子通量影响过度渗透应激反应的过程； （iv）分析类囊体离子通量对作物植物中关键的农艺特征的影响； （v）生成一个模型，用于模拟光合作用效率和盐胁迫抗性，这是类囊体离子通量的函数。 Flux4lives项目具有更广泛的影响。光合作用可以说是地球上最重要的反应，也是生命的基础。有明确的证据表明该领域的生长状况最近变得越来越不利，因此对非生物应力如何影响该反应途径有详细的理解。了解保护光合作用和植物生产力的分子​​机制将是改善这些途径，从而确保必要水平的全球粮食生产水平的关键。由于获得的数据将通过现有的Photosynq数据库开放，因此全球科学界可以将知识应用于其自身的生物学和生态问题，从而进一步努力满足各种气候和条件的食物需求。这奖反映了NSF的法规使命，并认为通过基金会的知识优点和广泛的评论，可以通过评估来进行评估，并获得了支持。

项目成果

Growth under Fluctuating Light Reveals Large Trait Variation in a Panel of Arabidopsis Accessions

• DOI: 10.3390/plants9030316
• 发表时间: 2020-03-01
• 期刊: PLANTS-BASEL
• 影响因子: 4.5
• 作者: Kaiser, Elias;Walther, Dirk;Armbruster, Ute
• 通讯作者: Armbruster, Ute

Light Potentials of Photosynthetic Energy Storage in the Field: What limits the ability to use or dissipate rapidly increased light energy?

现场光合能量储存的光势：是什么限制了使用或消散快速增加的光能的能力？

• DOI: 10.1101/2021.08.26.457798
• 发表时间: 2021
• 期刊: bioRxiv
• 作者: Kanazawa, Atsuko;Chattopadhyay, Abhijnan;Kuhlgert, Sebastian;Tuitupou, Hainite;Maiti, Tapabrata;Kramer, David M.
• 通讯作者: Kramer, David M.

Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis in Arabidopsis

光驯化与类囊体离子运输相互作用，控制拟南芥光合作用的动态

• DOI: 10.1111/nph.18534
• 发表时间: 2023
• 期刊: New Phytologist
• 影响因子: 9.4
• 作者: von Bismarck, Thekla;Korkmaz, Kübra;Ruß, Jeremy;Skurk, Kira;Kaiser, Elias;Correa Galvis, Viviana;Cruz, Jeffrey A.;Strand, Deserah D.;Köhl, Karin;Eirich, Jürgen
• 通讯作者: Eirich, Jürgen

Impact of ion fluxes across thylakoid membranes on photosynthetic electron transport and photoprotection

• DOI: 10.1038/s41477-021-00947-5
• 发表时间: 2021-06-17
• 期刊: NATURE PLANTS
• 影响因子: 18
• 作者: Li, Meng;Svoboda, Vaclav;Kirchhoff, Helmut
• 通讯作者: Kirchhoff, Helmut