MRI: Acquisition of a laser scanning confocal microscope within a core facility for research and training at Illinois State University

MRI：在伊利诺伊州立大学的研究和培训核心设施内购置一台激光扫描共焦显微镜

• 批准号: 1828136
• 负责人: Kevin Edwards
• 金额: $ 66.13万
• 依托单位: Board of Trustees of Illinois State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828136&HistoricalAwards=false
• 关键词: MRI; Acquisition; laser; scanning; confocal

An award is made to Illinois State University (ISU) to acquire a multichannel laser scanning confocal microscope. The instrument will provide advanced imaging capabilities to ISU students and faculty in Physics, Chemistry, and Biological Sciences, as well as institutions across the Midwest region. As the centerpiece of the ISU Biological Sciences Microscopy Core Facility, the microscope will greatly enhance ISU's research infrastructure, enabling high-resolution three dimensional reconstructions of cells and tissues, time-lapse imaging of dynamic cellular processes in live samples, and advanced fluorescence techniques. ISU's diverse population of undergraduates will be able to use the facility as part of mentored independent research projects; over 50 undergraduate researchers per year are projected to learn widely useful STEM skills through this training. K-12 students will be exposed to concepts in cells, tissues, light microscopy, biotechnology, and neuroscience, through several ongoing outreach programs such as the Illinois Summer Research Academy. Students from groups underrepresented in science will be recruited for training via ISU's Louis Stokes Alliance for Minority Participation. Future and current K-12 science teachers will train on the instrument to gain practical research experience, for example in the NSF-funded project "Noyce Scholarships for STEM Teachers of Under-Represented Groups". 20-30 graduate students/year will use the facility to pursue new avenues of research, ultimately contributing new skills to the Illinois STEM workforce. Positive societal impacts of the project also include agricultural and environmental advances. It will promote the USDA-funded development of pennycress as a new winter cover crop, reducing soil erosion and nitrogen runoff from barren fields while profitably producing oilseed for generating biofuels.The microscope will support the projects of seven main research labs, advancing knowledge in genetics, cell biology, development, neuroscience, and plant science. Projects include: regulation of protein quality control in response to aging and oxidative stress; interactions among proteins that control directional growth of plant cells; mechanisms of gene silencing and meiotic drive in fungi; effects of newly synthesized chemicals on parasitic Leishmania; how nematode worms detect and respond to magnetic fields; in vivo roles of the cell's cortical cytoskeleton in organismal development and tissue maintenance; and regulation of neural activity by sensory inputs and neuromodulators. New capabilities provided by the award will allow users to expand the scope of their research; the system will feature a broad range of excitation wavelengths; rapid scanning; high-sensitivity, low noise photon detectors; sub-200 nm spatial resolution; and fluorescence lifetime data. The users engage in nation-wide and international research collaborations, which will be expanded and strengthened by the added capabilities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

伊利诺伊州立大学 (ISU) 获得了获得多通道激光扫描共焦显微镜的奖励。该仪器将为 ISU 物理、化学和生物科学领域的学生和教师以及中西部地区的机构提供先进的成像功能。作为 ISU 生物科学显微镜核心设施的核心设备，该显微镜将极大增强 ISU 的研究基础设施，实现细胞和组织的高分辨率三维重建、活体样本中动态细胞过程的延时成像以及先进的荧光技术。 ISU 的不同本科生群体将能够使用该设施作为指导独立研究项目的一部分；预计每年将有超过 50 名本科生研究人员通过此培训学习广泛有用的 STEM 技能。 K-12 学生将通过伊利诺伊州夏季研究学院等多个正在进行的外展项目接触细胞、组织、光学显微镜、生物技术和神经科学的概念。来自科学领域代表性不足群体的学生将通过 ISU 的路易斯斯托克斯少数群体参与联盟招募接受培训。未来和现任 K-12 科学教师将使用该仪器进行培训，以获得实际研究经验，例如在 NSF 资助的项目“为代表性不足群体的 STEM 教师提供诺伊斯奖学金”中。每年 20-30 名研究生将利用该设施寻求新的研究途径，最终为伊利诺伊州 STEM 劳动力贡献新技能。该项目的积极社会影响还包括农业和环境进步。它将促进美国农业部资助的矮芥菜作为一种新的冬季覆盖作物的开发，减少贫瘠土地的土壤侵蚀和氮径流，同时有利地生产用于生产生物燃料的油籽。该显微镜将支持七个主要研究实验室的项目，推进遗传学知识、细胞生物学、发育、神经科学和植物科学。项目包括：调节蛋白质质量控​​制以应对衰老和氧化应激；控制植物细胞定向生长的蛋白质之间的相互作用；真菌基因沉默和减数分裂驱动机制；新合成的化学品对寄生利什曼原虫的影响；线虫如何检测磁场并做出反应；细胞皮质细胞骨架在生物体发育和组织维持中的体内作用；以及通过感觉输入和神经调节剂调节神经活动。该奖项提供的新功能将使用户能够扩大他们的研究范围；该系统将具有广泛的激发波长范围；快速扫描；高灵敏度、低噪声光子探测器；空间分辨率低于 200 nm；和荧光寿命数据。用户参与全国和国际研究合作，这些合作将通过增加的功能得到扩展和加强。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Drosophila p38 MAPK interacts with BAG‐3/starvin to regulate age‐dependent protein homeostasis

果蝇 p38 MAPK 与 BAG-3/starvin 相互作用调节年龄依赖性蛋白质稳态

• DOI: 10.1111/acel.13481
• 发表时间: 2021-11
• 期刊: Aging Cell
• 影响因子: 7.8
• 作者: Ryan SM;Almassey M;Burch AM;Ngo G;Martin JM;Myers D;Compton D;Archie S;Cross M;Naeger L;Salzman A;Virola-Iarussi A;Barbee SA;Mortimer NT;Sanyal S;Vrailas-Mortimer AD
• 通讯作者: Vrailas-Mortimer AD

Extracellular matrix protein N-glycosylation mediates immune self-tolerance in Drosophila melanogaster

细胞外基质蛋白 N-糖基化介导果蝇免疫自我耐受

• DOI: 10.1073/pnas.2017460118
• 发表时间: 2021-09-28
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Mortimer NT;Fischer ML;Waring AL;Kr P;Kacsoh BZ;Brantley SE;Keebaugh ES;Hill J;Lark C;Martin J;Bains P;Lee J;Vrailas-Mortimer AD;Schlenke TA
• 通讯作者: Schlenke TA

Distinct mechanoreceptor pezo-1 isoforms modulate food intake in the nematode Caenorhabditis elegans

独特的机械感受器 pezo-1 亚型调节线虫的食物摄入量

• DOI: 10.1093/g3journal/jkab429
• 发表时间: 2021-12
• 期刊: G3 Genes|Genomes|Genetics
• 作者: Hughes, Kiley;Shah, Ashka;Bai, Xiaofei;Adams, Jessica;Bauer, Rosemary;Jackson, Janelle;Harris, Emily;Ficca, Alyson;Freebairn, Ploy;Mohammed, Shawn;et al
• 通讯作者: et al

Temperature-robust activity patterns arise from coordinated axonal Sodium channel properties

温度稳健的活动模式源于协调的轴突钠通道特性

• DOI: 10.1371/journal.pcbi.1008057
• 发表时间: 2020-07
• 期刊: PLOS Computational Biology
• 作者: DeMaegd, Margaret L.;Stein, Wolfgang
• 通讯作者: Stein, Wolfgang

The dynamic range of voltage-dependent gap junction signaling is maintained by I h -induced membrane potential depolarization

电压依赖性间隙连接信号的动态范围由 I h 诱导的膜电位去极化维持

• DOI: 10.1152/jn.00545.2021
• 发表时间: 2022-03
• 期刊: Journal of Neurophysiology
• 影响因子: 2.5
• 作者: Stein, Wolfgang;DeMaegd, Margaret L.;Braun, Lena Yolanda;Vidal;Harris, Allison L.;Städele, Carola
• 通讯作者: Städele, Carola