SBIR Phase I: Enhancing the skin microbiome for mosquito repellency: Next generation mosquito repellent derived from big data analysis

SBIR 第一阶段：增强皮肤微生物群以实现驱蚊作用：基于大数据分析的下一代驱蚊剂

• 批准号: 1843179
• 负责人: Nicole Scott
• 金额: $ 22.5万
• 依托单位: Cybele Microbiome Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1843179&HistoricalAwards=false
• 关键词: SBIR; Phase; Enhancing; skin; microbiome

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project is to develop a safe, natural, long-lasting and effective mosquito repellent product by exploiting the natural repellent compounds present in the skin microbiome. Mosquito-borne illnesses affect 700M people worldwide and cause several million deaths each year. Chikungunya, West Nile, Malaria, Zika, Dengue, are just some of the debilitating vectors spread by mosquitoes. Mosquito-derived diseases cost $6.8B/year and the market for mosquito repellents is $3.2B and growing. Many solutions are aimed at killing and disabling mosquitoes, but mosquitoes are a crucial part of the ecological food chain. Further, many repellents are extremely toxic, and mosquitoes are developing resistance. The proposed technology will be used to identify compounds that will drive the endogenous skin microbiome to a more mosquito repellent phenotype. Using these methods, it is possible to harness the biochemistry contained in the native skin microbiome to create an endogenous repellency that is a safe, natural, environmentally friendly, and effective barrier to mosquitoes. It is expected that this repellency approach may be used safely even with children and pregnant women. In addition, it may be possible to use this approach to develop repellents for multiple insects, repellent products for companion animals, and additional over-the-counter skin products.The intellectual merit of this SBIR Phase I project is to develop a new class of topical insect repellents by influencing repellency in the skin microbiome. This will be accomplished by developing and validating a platform technology to identify compounds produced by the microbiome that are associated with biochemical pathways of interest, and use the identified compounds to target those pathways whose end-products comprise natural mosquito-repellent compounds. The goal is to create a topical mosquito repellent product. For this project, the plan is to complete metagenome sequencing on skin samples collected from individuals that are either naturally repellent or attractive to Anopheles mosquitoes (the mosquito that carries Malaria). The next step is to predict metabolite compound turnover and use metabolomics to validate the results. In vitro assays and in silico models will be used to provide proof-of-concept for the use of those predicted compounds to induce the microbiota to produce mosquito repellent metabolites.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）项目的更广泛的影响/商业潜力是通过利用皮肤微生物组中存在的天然驱虫剂化合物来开发安全，自然，持久和有效的驱虫产品。 蚊子 - 传播疾病影响着全球7亿人，每年造成数百万人死亡。 奇康古尼亚，西尼罗河，疟疾，寨卡，登革热只是蚊子传播的一些令人衰弱的媒介。蚊子衍生的疾病的价格为$ 6.8B/年，蚊子市场的市场为$ 3.2B且增长。许多解决方案旨在杀死和禁用蚊子，但蚊子是生态食物链的关键部分。此外，许多驱虫剂具有极高的毒性，蚊子正在发展抗性。提出的技术将用于识别将内源性皮肤微生物组驱动到驱虫剂表型的化合物。使用这些方法，可以利用天然皮肤微生物组中包含的生物化学，以产生一种安全，自然，环保且有效的蚊子屏障的内源性驱动性。预计即使与儿童和孕妇一起可以安全地使用这种驱除态度。此外，可能有可能使用这种方法来为多种昆虫，伴侣动物的驱虫产品以及其他非处方皮肤产品开发驱虫剂。该SBIR I期项目的智力优点是通过影响皮肤微生物组的不适来开发新的局部昆虫驱虫剂。 这将通过开发和验证平台技术来识别与生物化学途径相关的微生物组产生的化合物，并使用确定的化合物来针对那些末端产生的途径，其最终产物构成了天然蚊子重质化合物。目的是创建局部蚊子驱虫产品。对于这个项目，该计划是在从天然驱虫剂或对亚皮特蚊子（带有疟疾的蚊子）中收集的皮肤样品中完成元基因组测序。下一步是预测代谢产物化合物周转，并使用代谢组学来验证结果。体外测定和在计算机模型中将用于提供概念验证，用于使用这些预测的化合物来诱导微生物群生产蚊子驱虫剂代谢物。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和广泛的影响来评估Criteria的智力功能和广泛影响。