Unraveling the early events of the iron deficiency response at cell-specific resolution

以细胞特异性分辨率揭示缺铁反应的早期事件

基本信息

批准号：
1818312
负责人：
David Mendoza-Cozatl
金额：
$ 99.56万
依托单位：
University of Missouri-Columbia
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2023-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1818312&HistoricalAwards=false
关键词：
Unraveling early events iron deficiency

项目摘要

Award AbstractTitle: How plants sense iron: Unraveling the early events of the iron deficiency response at cell-specific resolution PI: David Mendoza-Cozatl, Univ. of Missouri, Columbia.Co-PIs: Scott Peck, Univ. of Missouri, Columbia; Dmitri Nusinow, Danforth Plant Science CenterIron is an essential nutrient for humans, and plants are the main dietary source of iron not only for humans but also for livestock. Iron deficiency in humans has been described by the World Health Organization as the most common nutritional deficiency affecting nearly 2.2 billion people (~30% of the world's population). Therefore, understanding how plants sense, take up and re-distribute iron in edible tissues is essential. Traditionally, iron sensing by plants was believed to occur exclusively by roots that are in close contact with iron sources in the soil, however, provocative preliminary data from our laboratories and others suggests that plants rapidly sense changes in iron concentrations in specialized cells located in leaves, termed companion cells. This project will focus on the early events of iron deficiency responses in plants and on the communication between leaves and roots to adapt when iron becomes scarce. In addition, this project will provide training to undergraduate and graduate students on cutting-edge molecular biology techniques, particularly techniques that address changes in specific tissues such as the veins of plants, which have been shown to play an important role in leaf-to-root communication. Moreover, this project will emphasize collaborative work between students from different disciplines including computer sciences, biochemistry and plant sciences. Learning how to communicate across disciplines is critical for developing novel techniques and instrumentation to study in detail how plants respond and adapt to changes in nutrient availability. In the long-term, students capable of understanding and bridging different disciplines will be well-equipped for the current competitive job market in academia and industry.The long-term goal of this project is the identification of molecular mechanisms mediating iron (Fe) sensing and homeostasis in plants. Respiration and photosynthesis heavily depend on the redox properties of iron to generate and store energy; however, this reactivity makes iron extremely toxic at high concentrations. Therefore, plants need to sense the levels of Fe within tissues and regulate Fe uptake to prevent overload and cellular damage. Despite significant advances in identifying molecular components of the Fe deficiency response in plants, the sensing and precise location of Fe sensing in plants remains unknown. More recently, it has been found that specific cells within the leaf vasculature (companion cells) are capable of sensing changes in iron availability more rapidly than roots. This project will use a cross-disciplinary approach to address the long-standing question about the spatial (where) and temporal (when) responses to iron deficiency in plants by identifying and integrating transcriptional and protein networks at cell-specific resolution. Several techniques, such as proximity labeling, have been specifically adapted to plants to pursue proteomic studies in specific tissues. Cell-specific translatome analyses during early stages of iron deficiency will be used to define the primary (early) and secondary (responses) to iron limitation in the vasculature and will guide high-throughput protein-DNA binding experiments to identify the molecular mechanisms driving early responses to Fe deficiency in companion cells. Experimental approaches will be complemented with modeling and simulation to produce an integrated view of plant responses to changes in iron availability.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

奖励摘要：植物感如何感知铁：在细胞特异性分辨率PI上阐明铁缺乏响应的早期事件：David Mendoza-Cozatl，Univ。密苏里州，哥伦比亚。哥伦比亚密苏里州； Dmitri Nusinow，Danforth Plant Science Centeriron是人类必不可少的营养素，植物不仅对人类，而且是牲畜的主要饮食来源。世界卫生组织已经将人类的铁缺乏症描述为影响近22亿人口的最常见的营养缺乏症（占世界人口的30％）。因此，了解植物在可食用组织中的感知，占用和重新分布铁是必不可少的。传统上，据信植物的铁感应仅是由与土壤中的铁源密切接触的根部发生的，但是，我们实验室的挑衅性初步数据表明，植物在叶片中迅速感知叶子中的铁浓度的变化，位于叶片中的特殊细胞中，称为伴随细胞。该项目将重点介绍植物中铁缺乏反应的早期事件，以及在铁变得稀缺时叶子和根之间的交流以适应。此外，该项目将向本科和研究生提供有关尖端分子生物学技术的培训，尤其是解决特定组织（例如植物静脉）的变化的技术，这些技术已被证明在叶子到叶子交流中起着重要作用。此外，该项目将强调来自不同学科的学生之间的合作工作，包括计算机科学，生物化学和植物科学。学习如何跨学科交流对于开发新型技术和仪器以详细研究植物如何反应并适应养分可用性的变化至关重要。从长远来看，能够理解和弥合不同学科的学生将为当前的学术界和行业竞争性就业市场提供良好的设备。该项目的长期目标是鉴定介导植物中铁（FE）感应和稳态的分子机制。呼吸和光合作用在很大程度上取决于铁的氧化还原特性产生和储存能量。但是，这种反应使铁在高浓度下具有极高的毒性。因此，植物需要感知组织内的FE水平并调节FE摄取以防止过载和细胞损伤。尽管在识别植物中FE缺乏反应的分子成分方面取得了重大进展，但植物中Fe感测的感应和精确的位置仍然未知。最近，已经发现，叶片脉管系统中的特定细胞（伴随细胞）能够比根更快地传感铁的可用性变化。该项目将使用跨学科的方法来解决有关空间（其中）和时间（何时）对植物中铁缺乏症的长期问题，通过识别和整合细胞特异性分辨率的转录和蛋白质网络。几种技术，例如接近性标记，已专门适用于植物以在特定组织中进行蛋白质组学研究。铁缺乏症的早期阶段的细胞特异性翻译组分析将用于定义脉管系统中铁限制的主要（早期）和次级（次要反应），并将指导高通量蛋白-DNA结合实验，以确定分子机制，使得分子机制促进了伴侣细胞中FE次缺乏症的早期反应。实验方法将与建模和仿真相辅相成，以产生对铁可用性变化的植物反应的综合视图。该奖项反映了NSF的法定任务，并且使用基金会的智力优点和更广泛的影响审查标准，被认为值得通过评估来支持。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Expression of a dominant‐negative AtNEET‐H89C protein disrupts iron–sulfur metabolism and iron homeostasis in Arabidopsis

拟南芥中显性负性 AtNEET-H89C 蛋白的表达会破坏铁硫代谢和铁稳态

DOI：
10.1111/tpj.14581
发表时间：
2020
期刊：
The Plant Journal
影响因子：
0
作者：
Zandalinas, Sara I.;Song, Luhua;Sengupta, Soham;McInturf, Samuel A.;Grant, DeAna G.;Marjault, Henri‐Baptiste;Castro‐Guerrero, Norma A.;Burks, David;Azad, Rajeev K.;Mendoza‐Cozatl, David G.
通讯作者：
Mendoza‐Cozatl, David G.

Class I TCP transcription factor AtTCP8 modulates key brassinosteroid-responsive genes

I 类 TCP 转录因子 AtTCP8 调节关键油菜素类固醇反应基因

DOI：
10.1093/plphys/kiac332
发表时间：
2022
期刊：
Plant Physiology
影响因子：
7.4
作者：
Spears, Benjamin J;McInturf, Samuel A;Collins, Carina;Chlebowski, Meghann;Cseke, Leland J;Su, Jianbin;Mendoza-Cózatl, David G;Gassmann, Walter
通讯作者：
Gassmann, Walter

Keep talking: crosstalk between iron and sulfur networks fine-tunes growth and development to promote survival under iron limitation

DOI：
10.1093/jxb/erz290
发表时间：
2019-08-15
期刊：
JOURNAL OF EXPERIMENTAL BOTANY
影响因子：
6.9
作者：
Mendoza-Cozatl, David G.;Gokul, Arun;Keyster, Marshall
通讯作者：
Keyster, Marshall

Editorial overview: Not everyone can become a cell biologist, but a great cell biologist can come from anywhere

编辑概述：不是每个人都可以成为细胞生物学家，但伟大的细胞生物学家可以来自任何地方

DOI：
10.1016/j.pbi.2023.102367
发表时间：
2023
期刊：
Current Opinion in Plant Biology
影响因子：
9.5
作者：
Mendoza-Cózatl, David G.
通讯作者：
Mendoza-Cózatl, David G.

Iron Availability within the Leaf Vasculature Determines the Magnitude of Iron Deficiency Responses in Source and Sink Tissues in Arabidopsis

拟南芥叶脉管系统内的铁利用率决定了源组织和库组织缺铁反应的程度

DOI：
10.1093/pcp/pcac046
发表时间：
2022
期刊：
Plant and Cell Physiology
影响因子：
4.9
作者：
Nguyen, Nga T.;Khan, Mather A.;Castro–Guerrero, Norma A.;Chia, Ju-Chen;Vatamaniuk, Olena K.;Mari, Stephane;Jurisson, Silvia S.;Mendoza-Cozatl, David G.
通讯作者：
Mendoza-Cozatl, David G.