REU Site: Molecular and Biochemical Analysis of Proteins

REU 网站：蛋白质的分子和生化分析

• 批准号: 1757748
• 负责人: James Forney
• 金额: $ 24.87万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1757748&HistoricalAwards=false
• 关键词: REU; Site; Molecular; Biochemical; Analysis

This REU Site award to Purdue University in West Lafayette, Indiana, will provide research training for eight students for ten weeks during the summers of 2018- 2020. The program provides advanced training in the analysis of protein function. It facilitates detailed understanding of experimental laboratory research, insights into protein biochemistry and appreciation for the broader context of research. Projects mentored by faculty in the Department of Biochemistry include analysis of enzyme mechanisms, post-translational protein modifications, proteomics, and protein-nucleic acid interactions studied in a wide range of biological contexts including cell cycle control, epigenetic gene regulation and plant development. A three-day orientation will introduce key concepts in protein biochemistry and experimental design. The remainder of the program will emphasize full-time research supplemented with weekly sessions on ethical conduct in research, professional communication and the graduate school application process. Students will present their results at a departmental symposium and a university-wide poster session. The program will recruit life science and chemistry undergraduates with at least four semesters of college who have an interest in research. Selection of participants will be based on academic performance, demonstrated interest in research, and the potential for outstanding research careers in the life sciences. It is anticipated that a total of 24 students, primarily from schools with limited research opportunities, will be trained in the program with additional emphasis on recruiting students from groups that are underrepresented in the life sciences. Students will learn how research is conducted, improve their scientific communication skills and many will present the results of their work at scientific conferences.A common web-based assessment tool used by all REU Site programs funded by the Division of Biological Infrastructure will be used to determine the effectiveness of the training program. Additional assessments on student poster presentations will be performed. Students will be tracked after the program in order to determine their career paths and will be asked to respond to an automatic email sent via the NSF reporting system. More information is available by visiting http://www.ag.purdue.edu/biochem/Pages/REU.aspx, or by contacting the PI (Dr. James Forney at [e-mail]).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 REU 站点授予印第安纳州西拉斐特的普渡大学，将在 2018 年至 2020 年夏季为 8 名学生提供为期十周的研究培训。该计划提供蛋白质功能分析方面的高级培训。它有助于详细了解实验实验室研究、深入了解蛋白质生物化学以及了解更广泛的研究背景。由生物化学系教师指导的项目包括酶机制分析、翻译后蛋白质修饰、蛋白质组学和在广泛的生物背景下研究的蛋白质-核酸相互作用，包括细胞周期控制、表观遗传基因调控和植物发育。为期三天的培训将介绍蛋白质生物化学和实验设计的关键概念。该项目的其余部分将强调全日制研究，并辅以每周一次的关于研究道德行为、专业沟通和研究生院申请过程的课程。学生将在院系研讨会和全校海报会议上展示他们的成果。该项目将招收至少完成四个学期大学学业且对研究感兴趣的生命科学和化学本科生。参与者的选择将基于学术表现、对研究的兴趣以及在生命科学领域杰出研究生涯的潜力。预计共有 24 名学生（主要来自研究机会有限的学校）将接受该计划的培训，并特别强调从生命科学领域代表性不足的群体中招收学生。学生将学习如何进行研究，提高他们的科学沟通技巧，许多人将在科学会议上展示他们的工作成果。由生物基础设施司资助的所有 REU 站点项目使用的通用网络评估工具将用于确定培训计划的有效性。将对学生海报展示进行额外评估。项目结束后将对学生进行跟踪，以确定他们的职业道路，并要求他们回复通过 NSF 报告系统发送的自动电子邮件。如需了解更多信息，请访问 http://www.ag.purdue.edu/biochem/Pages/REU.aspx，或联系 PI（James Forney 博士，[e-mail]）。该奖项反映了 NSF 的法定使命通过使用基金会的智力优点和更广泛的影响审查标准进行评估，并被认为值得支持。

项目成果

Oct4-Mediated Inhibition of Lsd1 Activity Promotes the Active and Primed State of Pluripotency Enhancers

Oct4 介导的 Lsd1 活性抑制促进多能性增强剂的活性和启动状态

• DOI: 10.1016/j.celrep.2019.11.040
• 发表时间: 2020-02
• 期刊: Cell Reports
• 影响因子: 8.8
• 作者: AlAbdi, Lama;Saha, Debapriya;He, Ming;Dar, Mohd Saleem;Utturkar, Sagar M.;Sudyanti, Putu Ayu;McCune, Stephen;Spears, Brice H.;Breedlove, James A.;Lanman, Nadia A.;et al
• 通讯作者: et al

Establishing the contribution of active histone methylation marks to the aging transcriptional landscape of Drosophila photoreceptors

• DOI: 10.1038/s41598-023-32273-5
• 发表时间: 2023-03-29
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: Jauregui-Lozano, Juan;McGovern, Sarah E.;Bakhle, Kimaya M.;Hagins, Arrianna C.;Weake, Vikki M.
• 通讯作者: Weake, Vikki M.

Dnmt3bas coordinates transcriptional induction and alternative exon inclusion to promote catalytically active Dnmt3b expression

Dnmt3bas 协调转录诱导和替代外显子包含以促进催化活性 Dnmt3b 表达

• DOI: 10.1016/j.celrep.2023.112587
• 发表时间: 2023-06-27
• 期刊: Cell Reports
• 影响因子: 8.8
• 作者: Dar, Mohd Saleem;Mensah, Isaiah K.;He, Ming;McGovern, Sarah;Sohal, Ikjot Singh;Whitlock, Hannah Christian;Bippus, Nina Elise;Ceminsky, Madison;Emerson, Martin L.;Tan, Hern J.;et al
• 通讯作者: et al