RUI: Collaborative Research: Molecular mechanisms of dendrite development, maintenance and plasticity: in vivo single-neuron analysis in C. elegans

RUI：合作研究：树突发育、维持和可塑性的分子机制：线虫体内单神经元分析

• 批准号: 1754986
• 负责人: Michele Lemons
• 金额: $ 23.96万
• 依托单位: Assumption College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-15 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754986&HistoricalAwards=false
• 关键词: RUI; Collaborative; Research; Molecular; mechanisms

Brain development involves the formation and fine-tuning of complex networks of connections between nerve cells. These connections occur at specialized sites of nerve cell contact, often formed on thorn-like structures (called spines) that protrude from the nerve cells. Precisely-controlled structural changes in spines are important for proper learning and memory. Some neuro-developmental disorders are the result of improper spine outgrowth, or a failure to properly maintain spines. These dysfunctions result in abnormalities in nerve cell communication that decrease learning and memory performance. Preliminary work for the present project has shown that connections between some nerve cells in the nematode worm Caenorhabditis elegans have spine-like structures, similar to those in the human brain. This project will investigate the mechanisms by which spines are formed during normal development, and maintained throughout life. The research uses C. elegans worms because of the powerful genetic tools developed in this species for identifying and understanding molecular pathways that control basic cell functions. By applying these tools to the mechanisms underlying spine outgrowth and maintenance, we expect to gain critical novel insights into the requirements for healthy brain function. Important insights into neurodevelopmental disorders may also be provided. This research project will also support the training of future scientists by providing mentored research experiences for undergraduate and graduate students, as well as local high school teachers. Recruitment for these activities will emphasize participants from under-represented groups in science.Most excitatory synapses in the mammalian brain occur at dendritic spines, which are small actin-rich membrane protrusions that house neurotransmitter receptors and other signaling machinery. Spines are essential structures in synaptic connectivity and plasticity, and underlie important processes of learning and memory. Despite recent progress in defining molecular mechanisms that regulate spine morphological changes, many questions about the basic biology of spine formation, maintenance and plasticity remain unanswered. The role of the extracellular matrix (ECM) in regulating spine development and maintenance is particularly poorly understood. The present studies will employ genetic tools available in the nematode worm Caenorhabditis elegans to identify and characterize molecular pathways involved in synapse development and plasticity. Preliminary work revealed that excitatory synaptic contacts onto C. elegans GABAergic neurons occur at spine-like protrusions. This collaborative project, incorporating expertise from the Lemons, Francis and B'nard labs, will investigate ECM-mediated mechanisms for guiding dendrite development and dynamics, as well as applying unbiased forward genetic screening to uncover novel cellular and molecular pathways that regulate dendritic spines in vivo.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

大脑发育涉及神经细胞之间连接的复杂网络的形成和微调。这些连接发生在神经细胞接触的专门部位，通常是在神经细胞突出的刺状结构（称为刺）上形成的。精确控制的刺结构变化对于适当的学习和记忆很重要。一些神经发展疾病是脊柱出生不当的结果，或者是无法正确维护脊柱的结果。这些功能障碍导致神经细胞通信中的异常，从而降低学习和记忆表现。本项目的初步工作表明，秀丽隐杆线虫中的某些神经细胞之间的连接具有类似脊柱的结构，类似于人脑中的结构。该项目将研究在正常发育过程中形成棘的机制，并在整个生命中保持。该研究使用秀丽隐杆线虫蠕虫，因为该物种中开发的强大遗传工具来识别和理解控制基本细胞功能的分子途径。通过将这些工具应用于脊柱生长和维护的基础机制，我们希望能够对健康大脑功能的需求获得重要的新颖见解。还可以提供对神经发育障碍的重要见解。该研究项目还将通过为本科生和研究生以及当地高中教师提供指导的研究经验来支持未来科学家的培训。这些活动的招募将强调科学中代表性不足的群体的参与者。哺乳动物大脑中的大多数兴奋性突触发生在树突状棘上，它们是富含肌动蛋白的小膜突起，可容纳神经递质受体受体和其他信号机械。刺是突触连通性和可塑性的重要结构，是学习和记忆的重要过程。尽管最近在定义调节脊柱形态变化的分子机制方面取得了进展，但有关脊柱形成，维护和可塑性基本生物学的许多问题仍未得到解答。细胞外基质（ECM）在调节脊柱发育和维持中的作用尤其了解。本研究将采用线虫蠕虫秀丽隐杆线虫中可用的遗传工具来识别和表征与突触发育和可塑性有关的分子途径。初步工作表明，在脊柱样突起时发生兴奋性突触接触GABA能神经元。这个合作项目纳入了柠檬，弗朗西斯和B'Nard Labs的专业知识，将调查ECM介导的机制，用于指导树突发展和动态，并应用公正的远期遗传筛查以揭示新的细胞和分子途径，以通过vivo进行评估，并通过nsf的代表来调节了nsf的代表。智力优点和更广泛的影响审查标准。

项目成果

Integrins Have Cell-Type-Specific Roles in the Development of Motor Neuron Connectivity

整合素在运动神经元连接的发展中具有细胞类型特异性的作用

• DOI: 10.3390/jdb7030017
• 发表时间: 2019
• 期刊: Journal of Developmental Biology
• 影响因子: 2.7
• 作者: Oliver, Devyn;Norman, Emily;Bates, Heather;Avard, Rachel;Rettler, Monika;Bénard, Claire Y.;Francis, Michael M.;Lemons, Michele L.
• 通讯作者: Lemons, Michele L.

CASE STUDY Phantom Limb Pain: Feeling Sensation from a limb that is No Longer Present and What it can Reveal About Our Brain Anatomy

案例研究幻肢痛：对不再存在的肢体的感觉以及它可以揭示我们的大脑解剖结构

• 发表时间: 2021
• 期刊: Journal of undergraduate neuroscience education
• 作者: Lemons, M.L.