Collaborative Research: Ecological and evolutionary impacts of disrupted transmission on host-microbiome associations

合作研究：传播中断对宿主-微生物组关联的生态和进化影响

基本信息

批准号：
1753993
负责人：
Jessica Metcalf
金额：
$ 2.95万
依托单位：
Princeton University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2021-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1753993&HistoricalAwards=false
关键词：
Collaborative Research Ecological evolutionary impacts

项目摘要

The microbiome living in or on an organism is critical to that organism's health. It influences everything from growth and development to behavior to disease susceptibility. How an organism gets the best possible microbiome during its development is key to its lifetime health and reproductive success. However, we know very little about how the processes by which microbiomes are transmitted among individuals affects organism health. This knowledge gap becomes especially important when building predictions about how interfering in the transfer of microbes between individuals might matter. For example, there has been discussion of how caesarean section birth might reduce the transmission of microbiota from mother to offspring, and in agriculture there are numerous common practices, such as sterilization of seeds or crop rotation, that likely impact the movement of plant-associated microbes across generations. This research project will focus on the consequences of differences in how tomatoes, a model agricultural crop, get their microbiomes. It will compare how vertical transmission, from parent to offspring, or horizontal transmission, among unrelated organisms, affects microbiome establishment, adaptation, and function. The outcomes of this work will be relevant to the development of agricultural practices that better incorporate the potentially useful role of the plant microbiome for crop yield. Undergraduate students from underrepresented groups will also be involved in the project, and researchers will also work with local teachers to create teaching materials based on the research. The proposed work uses a model tomato plant - phyllosphere (leaf associated) microbiome system to determine both the short term (ecological) and longer-term (evolutionary) consequences of interrupted microbiome transmission between generations on host-microbiome interactions. By manipulating the likelihood that seedlings will be colonized by microbiota coming from parental plants versus unrelated plants, the work will directly test the impact of transmission mode on microbiome successional dynamics, adaptation, and function. The combination of microbial experimental evolution, culture-independent sequencing, and metagenomic analyses will allow for a causal link to be established between microbiome transmission mode and the taxonomic and functional diversity of phyllosphere microbiota. The empirical work will be complemented by theory on how vertical microbiome transmission affects host-microbiome coevolution. Together, the work will determine the importance of vertical transmission in shaping host-microbiome interactions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生活在生物体中或生物体上的微生物组对于该生物的健康至关重要。它影响了从增长，发展到行为再到疾病易感性的一切。生物体在开发过程中如何获得最佳的微生物组是其一生的健康和生殖成功的关键。但是，我们对微生物组在个体之间传播的过程如何影响生物体的健康知之甚少。当建立对个人之间微生物转移的干扰可能重要的预测时，这种知识差距尤为重要。例如，已经讨论过关于剖腹产的出生如何减少菌群从母亲到后代的传播，而在农业中，有许多常见的做法，例如种子的灭菌或作物旋转，可能会影响植物相关的微生物在世代相传的运动。该研究项目将重点介绍西红柿模型农作物如何获得微生物的后果。它将比较在无关的生物中，从父型到后代或水平传播的垂直传播如何影响微生物组的建立，适应和功能。这项工作的结果将与农业实践的发展有关，这些农业实践更好地纳入了植物微生物组在作物产量中的潜在有用作用。来自代表性不足小组的本科生也将参与该项目，研究人员还将与当地教师合作，根据研究来创建教材。拟议的工作使用模型的番茄植物 - 植物层（叶片相关）微生物组系统来确定短期（生态）和长期（进化）的后果在宿主 - 微生物组相互作用上的微生物组中断的后果。通过操纵来自父母植物与无关植物的微生物植物殖民幼苗的可能性，这项工作将直接测试传播模式对微生物组的演变动力学，适应性和功能的影响。微生物实验进化，独立的测序和宏基因组分析的组合将允许在微生物组传递模式与植物层微生物的分类学和功能多样性之间建立因果关系。关于垂直微生物组如何影响宿主 - 微生物组协同进化的理论，经验工作将得到补充。这项工作将共同确定垂直传播在塑造宿主 - 微生物组相互作用中的重要性。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响评估标准通过评估来支持的。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Why Evolve Reliance on the Microbiome for Timing of Ontogeny?

DOI：
10.1128/mbio.01496-19
发表时间：
2019-09-01
期刊：
MBIO
影响因子：
6.4
作者：
Metcalf, C. Jessica E.;Henry, Lucas P.;Koskella, Britt
通讯作者：
Koskella, Britt

Successive passaging of a plant-associated microbiome reveals robust habitat and host genotype-dependent selection

DOI：
10.1073/pnas.1908600116
发表时间：
2020-01-14
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Morella, Norma M.;Weng, Francis Cheng-Hsuan;Koskella, Britt
通讯作者：
Koskella, Britt

Plant neighborhood shapes diversity and reduces interspecific variation of the phyllosphere microbiome