Linkage between the distribution and biochemical properties of RuBisCO and CODH enzymes and abiotic properties in thermally and chemically distinct deep-sea hydrothermal vent systems

RubisCO 和 CODH 酶的分布和生化特性与热学和化学特性不同的深海热液喷口系统中的非生物特性之间的联系

• 批准号: 231865409
• 负责人: Professorin Dr. Mirjam Perner
• 金额: --
• 依托单位: Forschungseinheit Marine Geosysteme
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/231865409?language=en
• 关键词: Linkage; between; distribution; biochemical; properties

The project aims at investigating the distribution and biochemical properties of key enzymes associated with autotrophic CO2 fixation pathways from thermally and chemically distinct hydrothermal deep-sea vent environments on the Mid-Atlantic Ridge. Within this scope we will focus on two key enzymes: the carbon monoxide dehydrogenase (CODH) of the reductive acetyl coenzyme A pathway and the ribulose-1,5-bisphosphate carboxylase (RuBisCOs) of the Calvin Benson Bassham cycle. These two pathways and respective key enzymes were chosen among the autotrophic CO2 fixation pathways because they are contrasting with respect to the energy (ATP) required for operating the autotrophic pathway, the temperature range of the organisms known to fix the CO2 autotrophically and the oxygen tolerance/sensitivity of the respective key enzymes. For the proposed study we have constructed five metagenomic fosmid libraries from abiotically distinct hydrothermal vents (energy-rich versus energy-poor, oxygen-rich versus oxygen-poor and high temperature versus low temperature) and have established function-based approaches to seek recombinant CODH and RuBisCOs from these metagenomic fosmid libraries. The successful search for recombinant CODHs and RuBisCOs in metagenomic libraries constructed from deep-sea hydrothermal vent samples will expand our knowledge of the interconnection between the distribution and biochemical properties of CODH and RuBisCOs and the abiotic parameters of the natural hydrothermal habitats.

该项目旨在研究与自养二氧化碳固定途径相关的关键酶的分布和生化特性，从中大西洋山脊上的热和化学上不同的热液深海通风环境。在此范围内，我们将重点关注两种关键酶：还原性乙酰辅酶A途径的一氧化碳脱氢酶（CODH）和Calvin Benson Bassham循环的Ribulose-1,5-双磷酸羧化酶（Rubiscos）。这两种途径和各自的关键酶是在自养二氧化碳固定途径中选择的，因为它们与操作自养途径所需的能量（ATP）形成鲜明对比，这是已知的生物体的温度范围，该生物体已知的二氧化​​碳自发性固定型自发性途径和均具有O oxygy的耐药性/敏感性/敏感性。在拟议的研究中，我们已经从非生物上不同的热液通风孔（能量富含能量的能量与能量损失，富含氧气的氧气与高温与低温与低温与低温与低温的方法）中构建了五个宏基因组泡沫库，并建立了基于功能的方法来寻求这些Metagenomic Fosmid forsmid forsmid forsmid库的重组Codh和Rubiscos。成功地搜索由深海水热样品构建的元基因组库中的重组CODH和RUBISCOS将扩大我们对Codh和Rubiscos的分布和生物化学性质之间的相互联系以及自然热液栖息地的鲁滨参数的了解。