Workshop: Best Practices for using Next Generation Sequencing (NGS) Datasets to determine Robust Evidence of Positive Selection and Convergent Evolution of Polar Organisms.

研讨会：使用下一代测序 (NGS) 数据集确定极地生物正向选择和趋同进化的有力证据的最佳实践。

基本信息

批准号：
1744877
负责人：
Scott Santagata
金额：
$ 4.93万
依托单位：
Long Island University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2021-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1744877&HistoricalAwards=false
关键词：
Workshop Best Practices using Next

项目摘要

This workshop aims to bring together a range of investigators to advance and coordinate cross-disciplinary approaches for determining genetic adaptations in polar organisms using datasets derived from Next Generation Sequencing of organisms DNA (genomes) and RNA (trasncriptomes). In particular, this workshop will focus on marine invertebrates as a broad taxonomic grouping and focus on the following efforts. 1) Establishing collaborative research activities that can test for genes under positive selection from diverse polar organisms using genomic and transcriptomic datasets. 2) evaluating current analytical methods for determining positively selected genes among diverse marine organisms 3) exploring current and novel methodologies for detecting genetic modifications acquired through convergent evolution in response to similar environmental conditions and 4) reviewing lab-based protocols for demonstrating the potential functions of candidate genes identified as being positively selected for by Antarctic environments. The workshops will also focus discussions and recommendations to the broader scientific community regarding techniques amenable to freezing or otherwise preserving samples for future sequencing inquires. The workshop coordinators will be advertised broadly and recruit underrepresented groups to participate. Following workshop activities a final report will be published on the outcomes of the efforts.

该研讨会旨在将一系列研究人员汇集，以推进和协调跨学科方法，以使用源自生物体下一代测序DNA（基因组）和RNA（trasncriptomes）的下一代测序的数据集来确定极性生物的遗传适应。特别是，该研讨会将专注于海洋无脊椎动物作为广泛的分类组，并专注于以下努力。 1）建立合作研究活动，可以使用基因组和转录组数据集从不同的极性生物中进行积极选择下测试基因。 2）评估目前的分析方法，用于确定各种海洋生物之间的正面选择基因3）探索当前和新型方法，用于检测通过收敛性进化获得的遗传修饰，以响应类似的环境条件，4）审查基于实验室的方案，以证明候选基因的潜在功能被鉴定为鉴定为正面环境的潜在功能。这些研讨会还将针对更广泛的科学界的讨论和建议，讨论可冻结或以其他方式保存样本以进行未来测序询问的技术。研讨会协调员将被广泛宣传，并招募代表性不足的团体参加。在研讨会活动之后，将发布有关努力结果的最终报告。