EAGER: Bio-Inspired Electrochemical Sensing of Small Molecules using Antibodies

EAGER：使用抗体对小分子进行仿生电化学传感

基本信息

批准号：
1740961
负责人：
Edgar Goluch
金额：
$ 7.5万
依托单位：
Northeastern University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1740961&HistoricalAwards=false
关键词：
EAGER Bio Inspired Electrochemical Sensing

项目摘要

PI: Goluch, Edgar D. Proposal Number: 1740961 A new approach for measuring small biological molecules such as toxins found in surface water is proposed. The approach, if successful, can provide a basis for compact devices that are portable for measuring harmful molecules. There is a scarcity of sensors for detection of small biological molecules. The current approach - enzyme-linked immunosorbent assay (ELISA) - generally cannot be used for sensing small biomolecules due to limited binding sites on the antigen. An alternative approach is needed. Antibodies that have antigens bound to them are recognized in mammalian systems by C1q protein. It is generally accepted that all antibodies undergo a conformational change when they bind to an antigen. The change is recognized by the protein, C1q, which attaches to the conserved, Fc, region of the antibody. In the proposed work, C1q recognition event will be used to examine feasibility of detecting small bioanalytes in aqueous samples. The binding measurements and characterization will be performed using commercially available instrument systems. In the second aim, C1q protein will be modified with a redox-active molecule for measuring using voltammetry-based electrochemical sensor, as it is a simple, robust, and mature technology. If the project is successful, it is expected to be broadly applicable to many sensing application with the potential to develop a low-cost, portable electrochemical sensing platform. The project will provide undergraduate and graduate students with multidisciplinary research experiences that will foster collaboration between engineers and biologists, preparing them for future workplace environments.

PI：Goluch，Edgar D。提案编号：1740961 提出了一种测量小生物分子（例如地表水中发现的毒素）的新方法。该方法如果成功，可以为便携式测量有害分子的紧凑型设备提供基础。用于检测小生物分子的传感器非常缺乏。目前的方法——酶联免疫吸附测定（ELISA）——由于抗原上的结合位点有限，通常不能用于感测小生物分子。需要一种替代方法。在哺乳动物系统中，C1q 蛋白可识别与抗原结合的抗体。人们普遍认为，所有抗体在与抗原结合时都会发生构象变化。这种变化被蛋白质 C1q 识别，该蛋白质附着在抗体的保守 Fc 区域上。在拟议的工作中，C1q 识别事件将用于检查检测水样中小生物分析物的可行性。结合测量和表征将使用市售仪器系统进行。在第二个目标中，C1q 蛋白将用氧化还原活性分子进行修饰，以便使用基于伏安法的电化学传感器进行测量，因为它是一种简单、稳健且成熟的技术。如果该项目成功，预计将广泛适用于许多传感应用，并有可能开发出低成本、便携式电化学传感平台。该项目将为本科生和研究生提供多学科研究经验，促进工程师和生物学家之间的合作，为他们未来的工作环境做好准备。