MRI: Acquisition of a high speed multiphoton laser-scanning microscope for research and training at UNLV

MRI：购买高速多光子激光扫描显微镜，用于 UNLV 的研究和培训

• 批准号: 1726925
• 负责人: Laurel Raftery
• 金额: $ 99.86万
• 依托单位: University of Nevada Las Vegas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726925&HistoricalAwards=false
• 关键词: MRI; Acquisition; speed; multiphoton; laser

An award is made to the University of Nevada, Las Vegas (UNLV) to acquire a high-speed multiphoton laser scanning microscope system to view the behaviors of cells in living tissues with time-lapse images. UNLV is the only research-intensive university in southern Nevada, and the largest in the state. Expansion of the scope and impact of UNLV research activities is a high priority for the State of Nevada, and part of its plan to diversify the economy beyond the gaming and tourism industries. The UNLV Confocal and Biological Imaging Core provides training and support for laser-scanning fluorescence microscopy in a wide array of biological, environmental, and engineering applications. Graduate students from across UNLV and nearby post-graduate professional universities receive basic and advanced training in quantitative fluorescence microscopy techniques, advancing the national goal of developing a diverse STEM workforce. Graduate students use the majority of Core instrument time, and are assisted by collaborating undergraduate researchers. In additional, other undergraduate UNLV students will experience the new multiphoton technology through modules in advanced laboratory coursework. In 2015, UNLV was ranked as one of the 10-most diverse public universities in the US. UNLV is also a Minority-Serving Institution for Native American and Pacific Islander students and an emerging Hispanic Serving Institution. Tours and demonstrations will be provided to students from nearby high schools and two-year and four-year colleges through outreach activities. To further recruit a diverse range of students to STEM disciplines, videos showing the inner workings of plant and animal tissues will be developed and presented in Clark County K-12 classrooms. Similar presentations for Science Café Las Vegas will enhance scientific literacy by introducing the lay public to the fascinating behaviors of cells within living tissues. The newly acquired multiphoton laser scanning microscope provides advanced technology to obtain images from living tissues to depths of 1 mm. This instrument will be located in the UNLV Confocal and Biological Imaging Core, so that it will be available on a first-come, first-served basis to scientists and students across Nevada. Availability of this instrument eliminates the barrier of transporting samples to other states, and enables Nevada researchers to study living systems at new levels of sensitivity and depth. The high-speed imaging technology decreases tissue damage and increases sensitivity, allowing detection of fast-changing processes over periods of seconds to hours. This type of imaging is driving unexpected discoveries about the brain and how cells work together to build the architecture of many other organs. The advanced capabilities of multiphoton laser scanning microscopy will be used to study a wide range of biological and engineering systems, such as hormonal control of gland secretion, electrical oscillations in living mouse brain slices, repair and regeneration of eye tissues, organization of bio-fouling bacteria, and electrical activity of polymers for robotics and nanotechnology.

向内华达大学拉斯维加斯大学（UNLV）颁发了奖励，以获取高速多光子激光扫描显微镜系统，以通过延时图像来查看活细胞中细胞的行为。 UNLV是内华达州南部唯一的研究密集于大学，也是该州最大的大学。扩大UNLV研究活动范围和影响的范围和影响是内华达州的一个很高的优先事项，也是它在游戏和旅游业以外的经济多样化的一部分。 UNLV共聚焦和生物成像核心为各种生物学，环境和工程应用中的激光扫描荧光显微镜提供了训练和支持。来自UNLV的研究生和研究生专业大学接受定量荧光显微镜技术的基本和高级培训，促进了发展潜水员STEM劳动力的国家目标。研究生使用大部分核心乐器时间，并通过合作的本科研究人员协作。另外，其他本科生UNLV学生将通过高级实验室课程中的模块体验新的Multiphoton技术。在2015年，UNLV被评为美国10个最潜水员的公立大学之一。 UNLV还是美国原住民和太平洋岛民学生和新兴的西班牙裔服务机构的少数派服务机构。通过外展活动，将向来自高中附近的学生以及两年和四年制大学的学生提供游览和示威游行。为了进一步招募潜水员的学生来茎学科，将在Clark County K-12教室开发和展示显示动植物组织内部工作的视频。科学咖啡馆的类似演讲将通过向外行公众引入活组织中细胞的迷人行为来增强科学素养。新获得的多光子激光扫描显微镜提供了先进的技术，可以从活组织到1毫米的深度获取图像。该仪器将位于UNLV共聚焦和生物成像核心中，因此它将以先到先得的基础向内华达州的科学家和学生提供。该工具的可用性消除了将样品运输到其他州的障碍，并使内华达州的研究人员能够以新的灵敏度和深度研究生活系统。高速成像技术可降低组织损伤并提高灵敏度，从而在几秒钟到几个小时内检测到快速变化的过程。这种类型的成像正在推动有关大脑以及细胞如何共同构建许多其他器官的结构的意外发现。多光子激光扫描显微镜的高级功能将用于研究广泛的生物学和工程系统，例如对腺体分泌的马控制，活着的小鼠脑切片中的电振荡，眼组织的修复和再生，生物结婚细菌的组织以及对机器人和Nananotechnolotic和Nananotechnolotic和Nananotechnolotic和Nananotechnotechnolotic和Nananotechnotechnolotic和Nananotechnolotics和Nananotechnotechnolotics和Nananotechnolotics的电活动的组织。