MRI Consortium: Acquisition of a Leica TCS SP8 STED 3X Super Resolution microscope

MRI 联盟：购买 Leica TCS SP8 STED 3X 超分辨率显微镜

基本信息

批准号：
1725984
负责人：
Chaitanya Ullal
金额：
$ 103.06万
依托单位：
Rensselaer Polytechnic Institute
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2020-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1725984&HistoricalAwards=false
关键词：
MRI Consortium Acquisition Leica TCS

项目摘要

An award is made to Rensselaer Polytechnic Institute (RPI) to purchase a super-resolution microscope (Leica STED) that will serve as the super-resolution center in the New York Capital Region. This project will impact many layers of our society. First, information about STED microscopy and its applications will be incorporated in several courses (Methods in Biophysics BCBP4800-RPI; Bio513 Modern Use of Light Microscopy-SUNY; Bioimaging AMC 608; Learn, Design, Teach Nanotechnology RPI MTLE 4960; Microscopy and Analysis "Tools"-Wadsworth Center). It will be part of the CBIS Annual Hands-On Workshops. It will be part of the Biological Sciences Seminar Series at all four institutions. Second, it will be incorporated in community outreach programs to encourage students into STEM fields: Black Family Technology Awareness Day; the High School Summer Research Program and the LSAMP Summer Research Program; the annual Science, Technology, Arts, and Architecture at Rensselaer Program that reaches out to talented underrepresented minorities and young women. Third, information about this technology and the research that it enables, including in nontraditional fields, will be distributed throughout the region's biotechnology community via Bioconnex, a network dedicated to the development of partnerships between research, education and business in the NY Capital region. Fourth, one of the RPI "Public Interest Research Forums" will be dedicated to STED and will establish the connection between this cutting-edge technology, the Nobel prize and implications to the general public.The intellectual merit of this project is the research activities that are enabled through the application of simulated emission depletion (STED) microscopy. STED microscopy creates super-resolution images by the selective deactivation of fluorophores, minimizing the area of illumination, and thus, bypassing the diffraction limit of conventional light microscopy. The microscope will enable very interdisciplinary research: 25 investigators across four institutions are strongly supporting this application, representing fourteen different Departments/Centers. Areas of research enabled include the study of cellular adherens and cellular permeability, STEM cell research, smooth muscle cell development, intracellular molecular interactions, endothelial cell development, and functional characterization of presynaptic terminals at small central synapses. The critical need for this technology and the diverse research applications for the instrument demonstrate that the super resolution microscope will become an extraordinary regional resource. Knowledge accumulated as a result of using STED imaging will have a tremendous positive impact in the fields of biological sciences, materials characterization and tissue engineering in the greater New York Capital Region.

向伦斯勒理工学院（RPI）颁发了奖励，以购买将作为纽约首都地区的超分辨率中心的超分辨率显微镜（Leica Sted）。该项目将影响我们社会的许多层面。首先，有关STED显微镜及其应用的信息将纳入多个课程（生物物理学BCBP4800-RPI中的方法； Bio513光学显微镜 - 季节的现代使用； AMC 608；学习，设计，设计，设计，教授纳米技术RPI RPI RPI MTLE 4960; MICROSCOPY; MICROSCOPY＆MICROSCOPY and ANANESSION和ANANESSION SRANESSION''工具“ -wadorth” -Wadorth'-WADSSR中心）。它将是CBIS年度动手讲习班的一部分。在所有四个机构中，它将成为生物科学研讨会系列的一部分。其次，它将被纳入社区外展计划中，以鼓励学生进入STEM领域：黑人家庭技术意识日；高中夏季研究计划和LSAMP夏季研究计划； Rensselaer计划的年度科学，技术，艺术和建筑，该计划与有才华的少数族裔和年轻妇女接触。第三，有关该技术及其启用的研究的信息，包括在非传统领域，将通过Bioconnex在整个地区的生物技术社区中分发，该网络致力于纽约首都地区的研究，教育和商业之间的合作伙伴关系。第四，RPI的“公共利益研究论坛”之一将致力于Sting，并将建立这种尖端技术，诺贝尔奖和与公众的影响之间的联系。该项目的智力优点是通过应用模拟排放耗尽（Sted）微观镜像来实现的研究活动。 Sted显微镜通过选择性失活的荧光团创造超分辨率图像，从而最大程度地减少了照明面积，从而绕过了常规光学显微镜的衍射极限。显微镜将实现非常跨学科的研究：四个机构的25名调查人员强烈支持这一应用，代表了14个不同的部门/中心。启用研究领域包括研究细胞粘着剂和细胞渗透性，干细胞研究，平滑肌细胞发育，细胞内分子相互作用，内皮细胞发育以及小中心突触下突触前末端的功能表征。对该技术的关键需求以及该仪器的多样化研究应用表明，超级分辨率显微镜将成为非凡的区域资源。由于使用Sted成像而积累的知识将对大纽约首都地区的生物科学，材料表征和组织工程领域产生巨大的积极影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Super-Resolution Imaging of Spatial Heterogeneities in Model Thermosensitive Hydrogels with Implications for Their Origins

热敏水凝胶模型中空间异质性的超分辨率成像及其起源的意义

DOI：
10.1021/acs.macromol.1c00754
发表时间：
2021
期刊：
Macromolecules
影响因子：
5.5
作者：
Kenath, Gopal S.;Karanastasis, Apostolos A.;Ullal, Chaitanya K.
通讯作者：
Ullal, Chaitanya K.

Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels

通过溶胀微凝胶的超分辨率显微镜定量功能交联剂反应动力学

DOI：
10.1039/c9sm01618j
发表时间：
2019
期刊：
Soft Matter
影响因子：
3.4
作者：
Karanastasis, Apostolos A.;Kenath, Gopal S.;Sundararaman, Ravishankar;Ullal, Chaitanya K.
通讯作者：
Ullal, Chaitanya K.

Rapid fabrication of collagen bundles mimicking tumor-associated collagen architectures