STTR Phase I: Microfluidic quartz resonator based blood plasma coagulation monitors

STTR 第一阶段：基于微流控石英谐振器的血浆凝血监测仪

• 批准号: 1721833
• 负责人: Zehra Parlak
• 金额: $ 22.5万
• 依托单位: Qatch Technologies LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-15 至 2018-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1721833&HistoricalAwards=false
• 关键词: STTR; Phase; Microfluidic; quartz; resonator

This STTR Phase I project aims to develop a novel microfluidic sensor technology that can measure blood coagulation times (specifically prothrombin time-PT, measured in international normalized ratio-INR) at point-of-care (POC). PT/INR has to be monitored frequently for millions of patients on oral Warfarin (an anticoagulant that prevents clotting) to keep them in a safe therapeutic range. The POC sensor developed in this project is expected to provide PT/INR measurements independent of factors influencing the blood counts of the patients, making it safer and more accurate. The technological foundation of the proposed research is to combine acoustic sensing with microfluidics. The preliminary data demonstrates that the proposed sensor can measure viscosity and density of extremely small liquid volumes (~ 10 nL) accurately. The successful implementation and commercialization of this innovation will result in a big market share in the rapidly growing POC coagulation test market and create hundreds of jobs. The research and development process during this STTR will also contribute to the full understanding of this technology?s potential, which can result in other self/home testing instruments for patients. The proposed novel coagulation monitor is based on microfluidic quartz resonator sensors. Quartz resonator sensors can be used to measure changes in fluid viscosity or mass coupled to their surfaces (both of which occur during coagulation of blood) by monitoring the associated changes in the resonance frequency. The technology underlying quartz resonators is well established, simple and robust, and amenable to provide portable and compact instrumentation. The innovation here is the integrated microfluidics, which will bring the benefits of extremely low liquid volume requirements, control over how liquid is observed by the sensor (which can be used to enhance sensor output due to the coagulation), and on-chip blood plasma separation. The key objectives of the proposed research are to determine the microfluidics design and the surface functionalization that will optimize a microfluidic quartz resonator sensor?s coagulation measurement sensitivity, and to implement on-chip blood plasma separation for PT/INR measurements.

该STTR I期项目旨在开发一种新型的微流体传感器技术，该技术可以测量凝血时间（特别是在国际标准化比率INR）（POC）（POC）上测量的血液凝血时间（特别是凝血酶原时间PT）。必须经常对数百万口腔华法林患者（一种防止凝结的抗凝剂）监测PT/INR，以使其保持安全的治疗范围。预计在该项目中开发的POC传感器将提供PT/INR测量值，而与影响患者血液计数的因素无关，使其更安全，更准确。拟议的研究的技术基础是将声学传感与微流体化合物结合起来。初步数据表明，所提出的传感器可以准确测量极小液体（〜10 nl）的粘度和密度。这项创新的成功实施和商业化将在快速增长的POC凝结测试市场中占有很大的市场份额，并创造了数百个就业机会。该STTR期间的研究和开发过程也将有助于对这项技术的潜力充分理解，这可能会为患者提供其他自我/家庭测试工具。提出的新型凝血监测器基于微流体石英谐振器传感器。石英谐振传感器可用于测量流体粘度的变化或通过监测共振频率的相关变化而与其表面耦合（两者都发生在血液凝结过程中）。石英谐振器基础的技术已经建立得很好，简单且健壮，并且可以提供便携式和紧凑的仪器。这里的创新是集成的微流体，它将带来极低的液体需求的好处，控制传感器观察到液体的观察方式（可用于增强由于凝结而提高传感器输出）和片上血浆分离。拟议研究的关键目的是确定微流体设计和表面功能化，以优化微流体石英谐振器传感器的凝结测量敏感性，并实现对PT/INR测量值的芯片血浆分离。