NPGI NSF Postdoctoral Fellowship in Biology FY 2017: Identifying the Genetic Determinants of Photosynthetic Pathways in Portulaca: Development of Germplasm and Genetic Resources

2017 财年 NPGI NSF 生物学博士后奖学金：识别马齿苋光合途径的遗传决定因素：种质和遗传资源的开发

基本信息

批准号：
1708941
负责人：
Eric Goolsby
金额：
$ 21.6万
依托单位：
Goolsby Eric W
依托单位国家：
美国
项目类别：
Fellowship Award
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2018-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1708941&HistoricalAwards=false
关键词：
NPGI NSF Postdoctoral Fellowship Biology

项目摘要

This action funds an NSF National Plant Genome Initiative Postdoctoral Research Fellowship in Biology for FY 2017. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The host institution for the fellowship is Brown University and the sponsoring scientist is Dr. Erika Edwards.All plants perform one of three types of photosynthesis. Two of these confer substantial stress tolerance, particularly under heat or drought stress. Most crops possess the third, less stress tolerant type of photosynthesis, and it is a goal of many breeding programs to give these crops more stress tolerance by changing the type of photosynthesis they perform. A major roadblock to doing this is a lack of knowledge of the complex genetic control that differentiates the three photosynthesis types. This work uses the unique genus Portulaca, a group of species whose members variously perform all three types of photosynthesis (and even shift among them), to better understand the genetic control of the anatomy and biochemistry underpinning the three types. PI Goolsby will be trained in modern approaches in plant genomics and physiology, and this work will provide tools for further research on improving crop photosynthetic efficiency and stress tolerance.This work will map the genetic architecture that differentiates C3, C4, and CAM photosynthesis in the diverse genus Portulaca. A mapping panel of recombinant inbred lines will be developed using a multiparent advanced generation intercross (MAGIC) approach from 8 founder lines spanning anatomical and biochemical extremes of C3, C4, and CAM photosynthesis within the genus. This panel will be sequenced to create a genetic map and phenotyped for key photosynthetic traits, which will allow association mapping and the identification of key candidate genes differentiating photosynthetic pathways. Germplasm and genetic data for the mapping panel will be deposited with the USDA and NCBI, respectively, providing open access to the scientific community and broader public and facilitating future basic and applied research efforts.

该行动资助了 2017 财年 NSF 国家植物基因组计划生物学博士后研究奖学金。该奖学金支持该研究员在主办实验室的研究和培训计划，该研究员还提出了一项扩大生物学参与的计划。该奖学金的主办机构是布朗大学，赞助科学家是埃里卡·爱德华兹博士。所有植物都进行三种光合作用中的一种。其中两种具有显着的胁迫耐受性，特别是在热或干旱胁迫下。大多数作物都具有第三种耐胁迫性较低的光合作用类型，许多育种计划的目标是通过改变这些作物进行的光合作用类型来赋予这些作物更高的胁迫耐受性。实现这一目标的一个主要障碍是缺乏对区分三种光合作用类型的复杂遗传控制的了解。这项工作使用了独特的马齿苋属，这是一组物种，其成员以不同的方式进行所有三种类型的光合作用（甚至在它们之间进行转换），以更好地了解支撑这三种类型的解剖学和生物化学的遗传控制。 PI Goolsby 将接受植物基因组学和生理学现代方法的培训，这项工作将为进一步研究提高作物光合作用效率和抗逆性提供工具。这项工作将绘制区分 C3、C4 和 CAM 光合作用的遗传结构。马齿苋属的不同属。重组自交系的绘图小组将使用多亲先进代杂交 (MAGIC) 方法从 8 个创始品系中开发，这些创始品系跨越该属内 C3、C4 和 CAM 光合作用的解剖学和生化极限。该面板将被测序以创建遗传图谱并针对关键光合性状进行表型分析，这将允许关联作图和识别区分光合途径的关键候选基因。绘图小组的种质和遗传数据将分别存放于美国农业部和 NCBI，为科学界和更广泛的公众提供开放获取，并促进未来的基础和应用研究工作。