CNIC: U.S.-Swedish Research on the Structure and Dynamics of Lipopolysaccharides (LPS)

CNIC：美国-瑞典关于脂多糖（LPS）结构和动力学的研究

• 批准号: 1664696
• 负责人: Wonpil Im
• 金额: $ 1.37万
• 依托单位: Lehigh University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1664696&HistoricalAwards=false
• 关键词: CNIC; U.S.; Swedish; Research; Structure

This one-year research initiation project through the Catalyzing New International Collaborations (CNIC) program links a new U.S.-Swedish team, led by Wompil Im at the University of Kansas and Göran Widmalm at Stockholm University, for the purpose of developing cooperative research on the structure and dynamics of lipopolysaccharides (LPS) using computational and experimental techniques. An LPS molecule is a complex amphiphatic compound consisting of lipid A, a core oligosaccharide, and an O-antigen polysaccharide with repeating units. These large and complex LPS molecules are vital to both the structural and functional integrity of gram-negative bacteria. The complexity of LPS makes them particularly challenging to study. With this support, the U.S.-Swedish team will combine expertise in both computational and NMR techniques to improve our understanding of the structure and dynamics of individual glycosidic sugar linkages and the impacts of the linkages on the overall LPS structures. Also of interest are the differences and similarities of LPS structure and dynamics in solution and bilayer environments. Peliminary answers to these important fundamental questions may have broader impact by contributing to future research yielding new knowledge of the biological impact of LPS molecules in mammals, such as the cause of fever, septic shock, or other deleterious physiological effects.Responding to recent recommendations in the 2012 book "Transforming Glycoscience: A Roadmap for the Future," published by The National Academies Press, the U.S. and Swedish partners also plan to integrate an education component with the research activities of the collaboration in the area of glycans, a general term for carbohydrates, sugars, and saccharides. In addition to providing graduate student training for one participant from the University of Kansas, the PI will involve two undergraduate students for early career experience with computational glycan biophysics. Therefore while abroad, each U.S. student should gain valuable exposure to an array of forefront computational and NMR techniques used by the U.S.-Swedish team.

这项为期一年的研究计划通过催化新的国际合作（CNIC）计划链接了由堪萨斯大学的Wompil IM和斯德哥尔摩大学的GöranWidmalm领导的一支新的美国 - 及子团队，目的是使用计算和实验技术来开发合作研究的合作研究。 LPS分子是一种复杂的两亲性化合物，由脂质A，核心寡糖和带有重复单元的O-抗原多糖组成。这些大而复杂的LPS分子对于革兰氏阴性细菌的结构和功能完整性至关重要。 LPS的复杂性使他们特别挑战学习。有了这一支持，美国宽度团队将结合计算和NMR技术方面的专家，以提高我们对单个糖苷糖链接的结构和动态的理解，以及链接对整体LPS结构的影响。同样有趣的是LPS结构的差异和相似性以及解决方案和双层环境中的动态。对这些重要的基本问题的骨气答案可能会通过为未来的研究做出贡献，从而对LPS分子在哺乳动物中的生物学影响的新知识做出新的了解，例如发烧，败血性休克的原因，或其他有害的生理效果，以应对2012年的《转变》的最新建议，以及未来的一本书。将教育组成部分与聚糖领域的合作研究活动相结合，碳氢化物，糖和糖的一般术语。除了为堪萨斯大学的一名参与者提供研究生培训外，PI还将涉及两名本科生，以获得计算聚糖生物物理学的早期职业经验。因此，在国外时，每个美国学生都应获得宝贵的接触，以了解美国 - 瑞典团队使用的一系列前列计算和NMR技术。