CAREER: Triggered Energy Transfer Chemiluminescence for In Vivo Imaging

职业：用于体内成像的触发能量转移化学发光

• 批准号: 1653474
• 负责人: Alexander Lippert
• 金额: $ 61.16万
• 依托单位: Southern Methodist University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1653474&HistoricalAwards=false
• 关键词: CAREER; Triggered; Energy; Transfer; Chemiluminescence

In this CAREER project funded by the Chemical Structure, Dynamics & Mechanisms B Program of the Chemistry Division, Professor Alexander R. Lippert of the Department of Chemistry at Southern Methodist University is developing responsive glow stick chemistry (chemiluminescence) for biological imaging. The project aims to synthesize and understand specialized molecules that spontaneously emit light in response to biological events. Mastering this fundamental chemical phenomenon found in fireflies and jellyfish provides new methods to measure and visualize the chemistry that lies at the heart of biological processes. The visual appeal of multi-colored chemiluminescence will be used to inspire future generations of students from K-12 to the graduate level with a centralized outreach theme of encouraging scientific creativity through seminars, K-12 research opportunities, and coursework aimed at teaching the creative process in a scientific context.Triggered chemiluminescence has the potential to dramatically transform biological imaging in wild-type organisms by eliminating autofluorescence, photobleaching, and other issues endemic to traditional fluorescence imaging techniques, but fundamental advances in understanding how molecular structure affects chemiluminescence emission is required. This project aims to synthesize, characterize, and understand new classes of triggered chemiluminescent imaging agents structurally designed to manipulate energy transfer processes. Fundamental questions to be addressed include: 1) How can the energy in a chemiluminescent excited state be efficiently transferred by through-space and through-bond energy transfer mechanisms to enhance luminescence emission? 2) What molecular structures will enable tuning of emission wavelengths for near-infrared and ratiometric readouts? 3) How can chemiluminescence emission be dynamically triggered with spatiotemporally controlled illumination to maximize signal to background in a region of interest?

在这个职业项目中由化学结构，动力学和机制B计划资助的化学部门，南方卫理公会化学系的Alexander R. Lippert教授正在开发用于生物成像的响应性发光棒化学（化学发光）。该项目旨在综合和理解专门的分子，这些分子自发地发出了对生物事件的响应。掌握在萤火虫和水母中发现的这种基本化学现象提供了测量和可视化生物过程核心的化学反应的新方法。多色化学发光的视觉吸引力将用于激发从K-12到研究生水平的子孙后代，其集中式外展主题是通过研讨会，K-12研究机会和课程旨在鼓励科学创造力，旨在教授创意在科学背景下的过程。触发的化学发光有可能通过消除自动荧光，光漂白和其他特有的传统荧光成像技术的特质，但在理解分子结构如何影响化学发光发射方面的基本进步，从而极大地改变野生型生物体的生物学成像，并具有基本进步。 。该项目旨在综合，表征和理解触发化学发光成像剂的新类别，旨在操纵能量传递过程。要解决的基本问题包括：1）化学发光激发态中的能量如何通过空间和循环能量传递机制有效地转移以增强发光发射？ 2）哪些分子结构可以为近红外和比率读数进行发射波长调整？ 3）如何通过空间控制的照明动态触发化学发光发射以最大化感兴趣区域的信号？

项目成果

Kinetics-Based Measurement of Hypoxia in Living Cells and Animals Using an Acetoxymethyl Ester Chemiluminescent Probe

• DOI: 10.1021/acssensors.9b00360
• 发表时间: 2019-05-01
• 期刊: ACS SENSORS
• 影响因子: 8.9
• 作者: Ryan, Lucas S.;Gerberich, Jeni;Lippert, Alexander R.
• 通讯作者: Lippert, Alexander R.

Ratiometric pH Imaging Using a 1,2-Dioxetane Chemiluminescence Resonance Energy Transfer Sensor in Live Animals

• DOI: 10.1021/acssensors.0c01393
• 发表时间: 2020-09-25
• 期刊: ACS SENSORS
• 影响因子: 8.9
• 作者: Ryan, Lucas S.;Gerberich, Jeni;Lippert, Alexander R.
• 通讯作者: Lippert, Alexander R.

Photoactivatable 1,2-dioxetane chemiluminophores

• DOI: 10.1016/j.rechem.2021.100106
• 发表时间: 2021-02
• 作者: L. S. Ryan;Andrew Nakatsuka;A. Lippert