Collaborative Research: Fabrication and Optimization of Continuous Stationary Phase Gradients for Liquid Chromatography

合作研究：液相色谱连续固定相梯度的制备和优化

• 批准号: 1609449
• 负责人: Maryanne Collinson
• 金额: $ 44.9万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1609449&HistoricalAwards=false
• 关键词: Collaborative; Research; Fabrication; Optimization; Continuous

This project is funded by the Chemical Measurement and Imaging Program of the Division of Chemistry at the National Science Foundation. The goal of a chemical separation is to separate a complex mixture into its individual components by taking advantage of differences in the degree of interaction a species exhibits between a stationary phase and a mobile phase. In this project, Professors Maryanne Collinson and Sarah Rutan at Virginia Commonwealth University (VCU) are exploring an alternate approach in the field of chemical separations. Instead of manipulating the composition of the mobile phase during the course of a separation, the stationary phase composition is instead changed. Gradual changes in stationary phases have many advantages including the ability to improve selectivity, separation times, and the ability to detect species in the mixture. The synergy between the collaborating research groups enhances the training of a diverse body of undergraduate and graduate students in state-of-the-art materials synthesis, chromatography, and the predictive power provided by simulations. In addition, by developing a peer-coaching relationship with new faculty, undergraduate instruction in analytical chemistry at VCU is being enhanced.This project focuses on the following three specific aims: (1) design, construction, and characterization of liquid chromatography columns with a continuous gradient in chemical functionality along their length, (2) development and implementation of tools to predict the chromatographic responses for various sets of analytes on a continuous gradient stationary phase, and (3) coupling simulations with experiments to determine what gradient lengths and compositions are needed to obtain optimum separations, thus avoiding the trial and error approach usually used in method development. For proof-of-principle, silica monolithic columns and columns packed with superficially porous particles are strategically modified to incorporate a continuous gradient in C8, C18, amine, and/or phenyl groups using silane chemistry and controlled rate infusion (CRI), a method recently developed in the Collinson group. The integration of the Collinson group's expertise in sol-gel chemistry and surface chemical gradients with the Rutan group's expertise in modeling of separations and liquid chromatography is leading to the development of new methodologies for problems of societal relevance, ranging from biomarker discovery to detection of low levels of environmental contaminants.

该项目由美国国家科学基金会化学部化学测量和成像项目资助。化学分离的目标是利用物质在固定相和流动相之间表现出的相互作用程度的差异，将复杂的混合物分离成单独的组分。在这个项目中，弗吉尼亚联邦大学 (VCU) 的 Maryanne Collinson 和 Sarah Rutan 教授正在探索化学分离领域的替代方法。在分离过程中，不是改变流动相的组成，而是改变固定相的组成。固定相的逐渐变化有许多优点，包括提高选择性、缩短分离时间以及检测混合物中物质的能力。 合作研究小组之间的协同作用增强了对不同本科生和研究生在最先进的材料合成、色谱和模拟提供的预测能力方面的培训。此外，通过与新教师建立同行辅导关系，弗吉尼亚联邦大学的分析化学本科教学正在得到加强。该项目重点关注以下三个具体目标：(1) 液相色谱柱的设计、构建和表征沿其长度的化学功能的连续梯度，(2) 开发和实施工具来预测连续梯度固定相上各组分析物的色谱响应，以及 (3) 将模拟与实验结合起来以确定梯度长度和组成需要获得最佳分离，从而避免了方法开发中通常使用的试错法。为了进行原理验证，使用硅烷化学和控制速率注入 (CRI) 方法对硅胶整体柱和填充表面多孔颗粒的柱进行了策略性修改，以在 C8、C18、胺和/或苯基中纳入连续梯度最近由 Collinson 小组开发。 Collinson 小组在溶胶-凝胶化学和表面化学梯度方面的专业知识与 Rutan 小组在分离建模和液相色谱方面的专业知识相结合，正在开发解决社会相关问题的新方法，从生物标志物发现到低浓度检测环境污染物的水平。