Collaborative Research: Phylogenetics of autoresistance in poison frogs as revealed by phylogenomics, neurophysiology, and comparative ecology

合作研究：通过系统基因组学、神经生理学和比较生态学揭示毒蛙自身抗性的系统发育学

基本信息

批准号：
1556967
负责人：
David Cannatella
金额：
$ 34.34万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2023-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1556967&HistoricalAwards=false
关键词：
Collaborative Research Phylogenetics autoresistance poison

项目摘要

Animals have evolved a variety of chemicals defenses against predators. A premier example is the toxic chemicals in the skin of poison frogs. Because these frogs are brightly colored, predators such as birds and snakes learn to avoid them. These toxins are alkaloids, found in many animals and plants. The frogs acquire these chemical defenses by eating ants and other arthropods that contain the alkaloids. These frog alkaloids (e.g., tetrodotoxin, batrachotoxin) are pharmacologically important and cause muscle paralysis, and disrupt breathing and cardiac function. At the cellular level, alkaloids affect ion channels, which control the transmission of nerve impulses. Surprisingly, the ion channels of poison frogs are are not affected by their own toxins; thus, deciphering how this evolved has important broader impacts that translate to human health. This research will use evolutionary trees to guide the activities: fieldwork to acquire and characterize natural alkaloids and collect ecological data on the species, molecular evolution of ion channel resistance to alkaloids, and neurophysiological effects of alkaloids on ion channels. The research will provide opportunities for students from the University of Texas and Indiana State University to jointly participate in international fieldwork, acquire new skills in bioinformatics, molecular modeling, genomics, and neurophysiology, as well as promote interactions between the two universities.The project will use Next-Generation DNA sequencing of transcriptomes, targeted sequencing of hundreds of genes, and bioinformatics methods to reconstruct evolutionary trees that will guide the research program with an experimental framework for explaining the origin of resistance, which has evolved multiple times in the poison frogs (family Dendrobatidae). Sequencing will also be used to identify novel amino acid replacements that may confer resistance to alkaloids. Computational modeling and in vitro assays of mutated ion channels will determine if these novel replacements provide resistance to natural and synthesized alkaloids. Fieldwork will focus on acquiring genetic samples, skin alkaloids, and color photographs from multiple populations of frogs, using natural history collection standards data collection and voucher specimens preparation. The combination of approaches will provide novel insights in the systematics of Dendrobatidae poison frogs, and also the evolution of auto-resistance to toxic alkaloids within the group.

动物已经进化了各种针对捕食者的化学药品防御。一个主要的例子是毒蛙皮中的有毒化学物质。由于这些青蛙的色彩鲜艳，因此鸟类和蛇等掠食者学会避免它们。这些毒素是许多动物和植物中发现的生物碱。青蛙通过吃蚂蚁和其他含有生物碱的节肢动物来获得这些化学防御。这些青蛙生物碱（例如，四毒素，巴氏毒素）在药理上很重要，引起肌肉麻痹，并破坏呼吸和心脏功能。在细胞水平上，生物碱会影响控制神经冲动的传播的离子通道。令人惊讶的是，毒蛙的离子通道不受其自己的毒素的影响。因此，破译这种演变如何具有转化为人类健康的重要广泛影响。这项研究将使用进化树来指导活动：获取和表征天然生物碱的现场工作，并收集有关物种的生态数据，离子通道对生物碱的耐药性的分子进化以及生物碱对离子通道的神经生理作用。这项研究将为来自德克萨斯大学和印第安纳州立大学的学生提供机会，共同参与国际野外工作，获得生物信息学，分子建模，基因组学和神经生理学的新技能，并促进两所大学之间的互动。这将通过一个实验框架来指导研究计划，以解释抗药性的起源，该抗药性在毒蛙（家庭dendrobatidae）中多次进化。测序还将用于鉴定可能赋予生物碱耐药性的新型氨基酸替代品。突变离子通道的计算建模和体外测定将确定这些新型替代品是否提供对天然和合成生物碱的抗性。现场工作将使用自然历史收集标准数据收集和代金券标本制备，从而获得来自多个青蛙种群的遗传样品，皮肤生物碱和彩色照片。方法的结合将提供在树突状毒蛙的系统学中的新见解，以及该组内自动抗性对毒性生物碱的演变。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Diversity within diversity: Parasite species richness in poison frogs assessed by transcriptomics

多样性中的多样性：通过转录组学评估毒蛙寄生虫物种丰富度

DOI：
10.1016/j.ympev.2018.03.015
发表时间：
2018
期刊：
Molecular Phylogenetics and Evolution
影响因子：
4.1
作者：
Santos, Juan C.;Tarvin, Rebecca D.;O'Connell, Lauren A.;Blackburn, David C.;Coloma, Luis A.
通讯作者：
Coloma, Luis A.

Convergent Substitutions in a Sodium Channel Suggest Multiple Origins of Toxin Resistance in Poison Frogs

钠通道中的趋同取代表明毒蛙毒素抗性的多个起源

DOI：
10.1093/molbev/msv350
发表时间：
2016
期刊：
Molecular Biology and Evolution
影响因子：
10.7
作者：
Tarvin, Rebecca D.;Santos, Juan C.;O'Connell, Lauren A.;Zakon, Harold H.;Cannatella, David C.
通讯作者：
Cannatella, David C.

The birth of aposematism: High phenotypic divergence and low genetic diversity in a young clade of poison frogs

警戒主义的诞生：年轻毒蛙进化枝的高表型分化和低遗传多样性

DOI：
10.1016/j.ympev.2016.12.035
发表时间：
2017
期刊：
Molecular Phylogenetics and Evolution
影响因子：
4.1
作者：
Tarvin, Rebecca D.;Powell, Emily A.;Santos, Juan C.;Ron, Santiago R.;Cannatella, David C.
通讯作者：
Cannatella, David C.