MRI: Acquisition of an Isothermal Titration Calorimeter for the Characterization of Molecular Interactions to Support Research and Undergraduate Education

MRI：购买等温滴定量热仪用于表征分子相互作用以支持研究和本科教育

• 批准号: 1531247
• 负责人: Veronica Moorman
• 金额: $ 9.16万
• 依托单位: Kettering University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531247&HistoricalAwards=false
• 关键词: MRI; Acquisition; Isothermal; Titration; Calorimeter

With this award from the Major Research Instrumentation (MRI) Program (MRI) and the Chemistry Research Instrumentation and Facilities (CRIF) Program, Kettering University will acquire an isothermal scanning calorimeter (ITC). Isothermal titration calorimetry has become a powerful technique for obtaining quantitative thermodynamic information about molecular interactions. It does so by sensitively measuring heat generated or absorbed by a compound upon titration using another compound. This leads to understanding the strength and structure of the interaction between the compounds. Binding between proteins, nucleic acids, lipids, small molecules, metals, nanoparticles, and polymers dissolved or suspended in aqueous or select non-aqueous solvents are accessible, making ITC extremely versatile. At Kettering University, the instrument will also be used by undergraduate students in their research projects. It will be incorporated into laboratory courses to provide training to students in several disciplines. It will be used in demonstrations and in hands on usage in outreach programs for middle and high school students. The proposal is aimed at enhancing research especially in areas such as (a) investigating protein-small molecule binding to explain the molecular evolution of the Yersina phosphatase YopH; (b) elucidating potential cytochrome c-dependent cardiovascular disease protection mechanisms for catechins, protein-metal complex binding; (c) determining the affinity of blood serum proteins for anti-cancer Ru(II)-mono-arene complexes; (d) determining protein-RNA binding for RNA sequence specificity of a T. brucei protein known to regulate gene expression and (e) studying small molecule-nanoparticle binding to understand the thermal properties of sodium acetate trihydrates for their use in non-electric incubating blankets.

凭借主要研究仪器 (MRI) 计划 (MRI) 和化学研究仪器和设施 (CRIF) 计划的这一奖项，凯特林大学将获得一台等温扫描量热计 (ITC)。等温滴定量热法已成为获得有关分子相互作用的定量热力学信息的强大技术。它通过灵敏地测量一种化合物在使用另一种化合物滴定时产生或吸收的热量来实现这一点。这有助于了解化合物之间相互作用的强度和结构。溶解或悬浮在水性或选定非水性溶剂中的蛋白质、核酸、脂质、小分子、金属、纳米粒子和聚合物之间的结合是可实现的，这使得 ITC 用途极其广泛。在凯特林大学，该仪器也将供本科生在他们的研究项目中使用。它将被纳入实验室课程，为多个学科的学生提供培训。它将用于演示以及针对中学生和高中生的外展项目的实际使用。该提案旨在加强研究，特别是在以下领域：(a) 研究蛋白质与小分子的结合，以解释耶尔森氏菌磷酸酶 YopH 的分子进化； (b) 阐明儿茶素、蛋白质-金属复合物结合的潜在细胞色素 c 依赖性心血管疾病保护机制； (c)测定血清蛋白对抗癌Ru(II)-单芳烃复合物的亲和力； (d) 确定已知调节基因表达的 T. brucei 蛋白的 RNA 序列特异性的蛋白-RNA 结合，以及 (e) 研究小分子-纳米颗粒结合，以了解三水乙酸钠的热特性，以用于非电孵化毯子。