FSML: ESTABLISHING A MODERN MOLECULAR DIAGNOSTICS FACILITY TO SUPPORT MICROBIOLOGY RESEARCH IN THE SIERRA NEVADA ECOREGION

FSML：建立现代分子诊断设施以支持内华达山脉生态区的微生物学研究

• 批准号: 1522430
• 负责人: Daniel Dawson
• 金额: $ 9.94万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1522430&HistoricalAwards=false
• 关键词: FSML; ESTABLISHING; MODERN; MOLECULAR; DIAGNOSTICS

Molecular diagnostics methods such as quantitative real-time PCR (qPCR) are increasingly important in environmental biology, including for the detection of pathogens and water quality assessment. Application of these methods is often hindered by access to the necessary equipment and instrumentation, especially in remote areas. California's Sierra Nevada is one of the best protected mountain ranges in the world, but nonetheless is affected by a host of environmental challenges, including impaired water quality. Although molecular diagnostics would greatly facilitate research on these problems and enable their remediation, no such facility exists in the area. This project will develop a molecular diagnostics facility at the University of California's Sierra Nevada Aquatic Research Laboratory (SNARL), located in the central Sierra Nevada. This facility will substantially enhance the research capabilities of SNARL. It will also have broader positive effects, including benefitting several land and resource management agencies with jurisdiction over the Sierra Nevada (National Park Service, California Department of Fish and Wildlife, California State Water Resources Control Board) whose need for molecular diagnostics has increased markedly in recent years. The facility will also expand teaching and training opportunities provided by existing undergraduate courses that use SNARL and facilitate the development of new courses, including those that increase engagement of students from the large populations of underrepresented groups that reside both locally and in southern California. The proposed facility also provides an opportunity to develop 'citizen science' projects aimed at high school students, college students, and the general public, including projects investigating water quality issues that directly impact local communities.The goal of this project is to add a molecular diagnostics facility to the existing microbiology laboratory at SNARL. This will include a qPCR system, large-capacity autoclave, biological safety cabinet, high-throughput tissue homogenizer, and micro-centrifuge. The proposed facility will be housed in the main laboratory building that was completely renovated and modernized in 2010-2011. The need for the proposed facility derived from a careful consideration of current and anticipated future research directions, an analysis of potential SNARL improvements and the projects they would benefit, and thorough consultation with SNARL users and the University of California management team. This new facility will dramatically expand the research capabilities of SNARL, including of the existing microbiology laboratory that is currently used primarily for bacterial culturing, and make available key research-related equipment that is either difficult to access or unavailable in the eastern Sierra Nevada. Sierra Nevada Aquatic Research Laboratory web site: http://vesr.nrs.ucsb.edu/

分子诊断方法（例如定量实时PCR（QPCR））在环境生物学中越来越重要，包括检测病原体和水质评估。这些方法的应用通常会因访问必要的设备和仪器，尤其是在偏远地区而受到阻碍。加利福尼亚的内华达山脉是世界上最好的受保护山脉之一，但仍受到许多环境挑战的影响，包括水质受损。尽管分子诊断将极大地促进这些问题的研究并实现它们的补救，但该地区没有这种设施。该项目将在加利福尼亚大学内华达大学内华达大学水生研究实验室（SNARL）开发一个分子诊断设施，该实验室位于内华达州中部。该设施将大大提高SNARL的研究能力。它还将具有更广泛的积极影响，包括在内华达山脉（国家公园管理局，加利福尼亚州鱼类和野生动物部，加利福尼亚州水资源控制委员会）中受益于几家土地和资源管理机构，其分子诊断的需求显着增加了近年来。该设施还将扩大现有的本科课程提供的教学和培训机会，这些课程使用SNARL并促进了新课程的发展，包括那些增加了来自当地和南加州居住的人群不足的团体人数不足的学生的参与度。拟议的设施还提供了一个机会，可以开发针对高中生，大学生和公众的“公民科学”项目，包括调查直接影响当地社区的水质问题的项目。该项目的目标是增加分子SNARL现有微生物实验室的诊断设施。这将包括QPCR系统，大容量高压灭菌器，生物安全柜，高通量组织均匀均匀的均匀剂和微中心。拟议的设施将安置在主要实验室大楼中，该建筑在2010 - 2011年进行了完全翻新和现代化。仔细考虑当前和预期的未来研究方向，对潜在的鼻涕改进及其将受益的项目的分析以及与SNARL用户和加利福尼亚大学管理团队进行彻底咨询的拟议设施的需求。该新设施将大大扩展SNARL的研究能力，包括现有的微生物学实验室，该实验室目前主要用于细菌培养，并提供与研究相关的关键研究，这些设备在内华达州东部很难获得或无法访问。内华达山脉水上研究实验室网站：http：//vesr.nrs.ucsb.edu/