UNS: Fractionation, purification, and analysis of gases in microbubbles

UNS：微泡中气体的分馏、纯化和分析

• 批准号: 1512544
• 负责人: Amar Basu
• 金额: $ 30.03万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1512544&HistoricalAwards=false
• 关键词: UNS; Fractionation; purification; analysis; gases

1512544BasuWayne State UniversityChemical separation, fractionation, and assay comprise an integral analytical workflow in life sciences research. When executing such workflows with liquid samples, conventional fraction collection tools such as pipettors, test tubes, and trays can be used. With gases, however, these basic operations are more challenging since gases are not easily contained. The objective of this proposal is to create a platform technology which can separate gases using gas chromatography (GC), and then and encapsulate them into bubbles so that they can be analyzed or manipulated offline. If successful, the proposed multiphase system would enable new workflows for gas purification and analysis analogous to their liquid counterparts. From the perspective of separation science, the encapsulation technique can encapsulate thousands of gas fractions at time resolution of 10ms and fraction volumes 1nL, roughly 1000X better than commercial preparatory-GC systems, which rely on cryogenic traps. This can can have transformative impact on prep-GC and multidimensional GC, where the resolution and scalability of fraction collection is critical. This platform would also provide a way to screen the reactivity of gas fractions by using chemical reagent assays which operate on samples in the bubble format. The proposed work will explore fundamental issues, including the containment of gases in a carrier fluid, and methods to analyze encapsulated gasses. From a broader perspective, this proposed system may substantially impact multidimensional gas analysis, a common workflow in environmental monitoring, homeland security, and food analysis. The outreach plan will deploy low-cost "jello" microfluidic kits at the Michigan Science center (MSC), with the goal of increasing interest in STEM-related disciplines in the Detroit area. Scientific knowledge from the proposed research will be integrated into the PI's graduate level BioMEMS and microfluidics course.

1512544Basuwayne State University化学分离，分级和测定包括生命科学研究中不可或缺的分析工作流程。 当使用液体样品执行此类工作流程时，可以使用传统的分数收集工具，例如移液器，试管和托盘。 但是，使用气体，这些基本操作更具挑战性，因为气体不容易含有。 该提案的目的是创建一种平台技术，该技术可以使用气相色谱法（GC）分离气体，然后将其封装成气泡，以便可以在离线分析或操纵它们。 如果成功，则提出的多相系统将使气体纯化和分析能够类似于其液体对应物。从分离科学的角度来看，封装技术可以在10ms的时间分辨率和1NL的分数时封装数千个气体分数，比依靠依靠低温堆的商业预备力-GC系统好1000倍。 这可以对Prep-GC和多维GC产生变革性的影响，在该GC中，分数收集的分辨率和可扩展性至关重要。该平台还将通过使用以气泡格式的样品操作的化学试剂分析来筛选气体分数的反应性的方法。拟议的工作将探讨基本问题，包括载体中的气体，以及分析封装气体的方法。从更广泛的角度来看，该提出的系统可能会显着影响多维气体分析，这是环境监测，国土安全性和食品分析的共同工作流程。外展计划将在密歇根州科学中心（MSC）部署低成本的“ Jello”微流体套件，目的是增加对底特律地区与STEM相关学科的兴趣。拟议研究的科学知识将纳入PI的研究生级生物元和微流体学课程中。