CAREER: "Holey Scaffold" Sensing System for Characterization of the Spatiotemporal Tumor Response to Nanoparticle-Mediated Photothermal and Photochemical Therapy

职业：“孔支架”传感系统，用于表征纳米粒子介导的光热和光化学疗法的时空肿瘤反应

• 批准号: 1505412
• 负责人: Marissa Rylander
• 金额: $ 27.94万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-10 至 2016-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1505412&HistoricalAwards=false
• 关键词: CAREER; Holey; Scaffold; Sensing; System

0955072RylanderThe goal of this CAREER plan is to create and utilize a novel sensing system called the "holey scaffold" to nondestructively and minimally invasively measure dynamic nanoparticle transport and photothermal and photochemical tumor response to nanoparticle-mediated laser therapy. A holey scaffold is a new technology co-invented by the PI that is composed of an embedded network of microchannels within a scaffold framework upon which cells and tissues can be grown. Hollow core fibers integrated in the holey scaffold permit simultaneous optical sensing/imaging of dynamic processes and selective delivery of biological agents (e.g. nutrients, cells, nanoparticles) for control of biochemical responses. The CAREER objective is to use holey scaffolds to measure 1) spatiotemporal nanoparticle transport for varying nanoparticle properties, targeting approaches, and delivery methods, 2) dynamic photothermal response (temperature, heat shock protein expression, and cell injury), and 3) real-time photochemical response (reactive oxygen species production) associated with nanoparticle-mediated laser therapies. The versatility of holey scaffolds will enable the first measurement of dynamic tumor response in vitro within a bioreactor system and in vivo in a mouse tumor model. Measured tumor response will be utilized to create a novel computational model for predicting tumor response and optimizing implementation of nanoparticles in laser therapy.

0955072Rylander该职业计划的目标是创建和利用一种名为“洞脚手架”的新型感应系统，以无损和微创测量动态纳米颗粒的传输以及光热和光化学肿瘤对纳米粒子介导的激光疗法的反应。孔支架是一项由PI共同发明的新技术，该技术由嵌入式微通道网络组成，在脚手架框架内可以在该框架上种植细胞和组织。集成在孔支架中的空心核纤维允许同时对动态过程的光学感应/成像以及对生物化学反应的控制生物学剂（例如营养，细胞，纳米颗粒）的选择性递送。 The CAREER objective is to use holey scaffolds to measure 1) spatiotemporal nanoparticle transport for varying nanoparticle properties, targeting approaches, and delivery methods, 2) dynamic photothermal response (temperature, heat shock protein expression, and cell injury), and 3) real-time photochemical response (reactive oxygen species production) associated with nanoparticle-mediated laser therapies.孔支架的多功能性将使在生物反应器系统和小鼠肿瘤模型中的体外进行首次测量动态肿瘤反应。测得的肿瘤反应将用于创建一种新的计算模型，以预测肿瘤反应并优化激光治疗中纳米颗粒的实现。